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The map label for this gene is ycdT [H]

Identifier: 118579321

GI number: 118579321

Start: 929047

End: 929904

Strand: Reverse

Name: ycdT [H]

Synonym: Ppro_0884

Alternate gene names: 118579321

Gene position: 929904-929047 (Counterclockwise)

Preceding gene: 118579323

Following gene: 118579320

Centisome position: 23.2

GC content: 56.29

Gene sequence:

>858_bases
ATGTTTGAGCGGATCCATCTGAAATTTGTTGCCATAGCATCACGGCACAGCAAACGCACCGTCGCGATCATAATCGGCCT
GATCCTGTCCCTGCTCTGCCTGCTCGATCACATTACCGGCGACTACTCGCTGACCATCTTTTACCTGATTCCGGTTTCCT
TAGGAGCATGGTTCGTAGGCAAGCCAGCCGGCATCTTTTTTTGCATACTGACCTTGGGTGCGCGATTCGCGGCCGACTAC
GGCTCTACTTCCGCAACGCGGAATTCAAGCCTGCATCACTGGAACCTCTTCATCGAGTTTTCATTCCTGATCGTCATGAG
CCTGCTCTCTTCGGCTCTCAAAACCAGGCTGGACAACGAAAAGGCCCTGGCCCGGATCGACCCCCTTACCGGCGCGATCA
ATCGCCGTTCCTTCTTCGACCTGGCAGAGCAGGAAATTTACCGGGCGCGCCGCTACGGACATTGCCTGACCATCGCCTAT
ATCGACCTGGACAACTTCAAGGAAGTCAACGACAGACAGGGACACTCCACGGGCGACAAGTTGCTCATTGCTGATGTCGA
CACCATCAGTGCCACCATCAGGAGCAGCGACATCCTGGCCCGCTTCGGCGGTGACGAATTCGTGATTCTCCTGCCGGAAA
CAGCCGGCGAGCCAGCCCAAACAACGCTCCACAAGGTGCAAAGCAAACTTCGGCAGTGCATGACAACGGGTAACTGGCCG
GTCACCTTCAGCATCGGCGCCATGAGCTACACGAGGCCGCCGGAAAGCGTCGAAGAGGCCGTGCGCAGCGCCGATGCACT
TATGTATGAGGTCAAGCGCAGCGGAAAGAACAGGCTGTTGCACATAACAACAGGGTAA

Upstream 100 bases:

>100_bases
TGGTTAGCCACGAGGTTTCCATACTCTGGCAACAGTCGATCCACATTCCGGGGACACACGTCCCCCTTTTCTTTTCGTGT
TGACTTGACAGGAAAAAGGC

Downstream 100 bases:

>100_bases
AAACGCCCTGCTCGCAAGGGAGAAACACCATGGCACGAGGCGGTTCCGACTTGAGTAACCTTATTGCGCCCCTGCTGATA
CGCCTGCCGCTGGGACTTAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MFERIHLKFVAIASRHSKRTVAIIIGLILSLLCLLDHITGDYSLTIFYLIPVSLGAWFVGKPAGIFFCILTLGARFAADY
GSTSATRNSSLHHWNLFIEFSFLIVMSLLSSALKTRLDNEKALARIDPLTGAINRRSFFDLAEQEIYRARRYGHCLTIAY
IDLDNFKEVNDRQGHSTGDKLLIADVDTISATIRSSDILARFGGDEFVILLPETAGEPAQTTLHKVQSKLRQCMTTGNWP
VTFSIGAMSYTRPPESVEEAVRSADALMYEVKRSGKNRLLHITTG

Sequences:

>Translated_285_residues
MFERIHLKFVAIASRHSKRTVAIIIGLILSLLCLLDHITGDYSLTIFYLIPVSLGAWFVGKPAGIFFCILTLGARFAADY
GSTSATRNSSLHHWNLFIEFSFLIVMSLLSSALKTRLDNEKALARIDPLTGAINRRSFFDLAEQEIYRARRYGHCLTIAY
IDLDNFKEVNDRQGHSTGDKLLIADVDTISATIRSSDILARFGGDEFVILLPETAGEPAQTTLHKVQSKLRQCMTTGNWP
VTFSIGAMSYTRPPESVEEAVRSADALMYEVKRSGKNRLLHITTG
>Mature_285_residues
MFERIHLKFVAIASRHSKRTVAIIIGLILSLLCLLDHITGDYSLTIFYLIPVSLGAWFVGKPAGIFFCILTLGARFAADY
GSTSATRNSSLHHWNLFIEFSFLIVMSLLSSALKTRLDNEKALARIDPLTGAINRRSFFDLAEQEIYRARRYGHCLTIAY
IDLDNFKEVNDRQGHSTGDKLLIADVDTISATIRSSDILARFGGDEFVILLPETAGEPAQTTLHKVQSKLRQCMTTGNWP
VTFSIGAMSYTRPPESVEEAVRSADALMYEVKRSGKNRLLHITTG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=33.5135135135135, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=284, Percent_Identity=28.8732394366197, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=205, Percent_Identity=30.2439024390244, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=32.1428571428571, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081977, Length=237, Percent_Identity=27.0042194092827, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788085, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=227, Percent_Identity=28.1938325991189, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=31.4814814814815, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=152, Percent_Identity=26.9736842105263, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=28.7425149700599, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=94, Percent_Identity=41.4893617021277, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787802, Length=184, Percent_Identity=24.4565217391304, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 31791; Mature: 31791

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERIHLKFVAIASRHSKRTVAIIIGLILSLLCLLDHITGDYSLTIFYLIPVSLGAWFVG
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHCCEECC
KPAGIFFCILTLGARFAADYGSTSATRNSSLHHWNLFIEFSFLIVMSLLSSALKTRLDNE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
KALARIDPLTGAINRRSFFDLAEQEIYRARRYGHCLTIAYIDLDNFKEVNDRQGHSTGDK
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCE
LLIADVDTISATIRSSDILARFGGDEFVILLPETAGEPAQTTLHKVQSKLRQCMTTGNWP
EEEEECHHHHHHHHCCHHHEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VTFSIGAMSYTRPPESVEEAVRSADALMYEVKRSGKNRLLHITTG
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MFERIHLKFVAIASRHSKRTVAIIIGLILSLLCLLDHITGDYSLTIFYLIPVSLGAWFVG
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHCCEECC
KPAGIFFCILTLGARFAADYGSTSATRNSSLHHWNLFIEFSFLIVMSLLSSALKTRLDNE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
KALARIDPLTGAINRRSFFDLAEQEIYRARRYGHCLTIAYIDLDNFKEVNDRQGHSTGDK
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCE
LLIADVDTISATIRSSDILARFGGDEFVILLPETAGEPAQTTLHKVQSKLRQCMTTGNWP
EEEEECHHHHHHHHCCHHHEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VTFSIGAMSYTRPPESVEEAVRSADALMYEVKRSGKNRLLHITTG
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]