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The map label for this gene is prfC

Identifier: 118579319

GI number: 118579319

Start: 926888

End: 928471

Strand: Reverse

Name: prfC

Synonym: Ppro_0882

Alternate gene names: 118579319

Gene position: 928471-926888 (Counterclockwise)

Preceding gene: 118579320

Following gene: 118579318

Centisome position: 23.17

GC content: 59.03

Gene sequence:

>1584_bases
GTGCAAAACCATAATCGCCAGGAAGTCGATAAACGTCGGACGTTTGCCATCATCAGCCACCCCGACGCAGGCAAGACCAC
CATCACCGAGAAGCTGCTGCTGTTCGGCGGCGCCATCCAGCAGGCGGGCGAGGTGCGGGCCAGGAAGTCGGCTCGCCACG
CCACATCGGACTGGATGGAACTAGAGAAGCAGCGCGGCATTTCGGTCACCTCATCGGTGATGAAATTCACCTACAACGGC
TGCGAAATCAACTTATTGGACACCCCCGGCCACAACGACTTTTCAGAGGACACCTACCGCGTGCTGACGGCGGTGGATTC
GGTGCTGATGGTGATCGACTCGGTCAAGGGGGTCGAATCCCAGACCAAAAAGCTGCTGGAAGTCTGCCGCCTGCGCCACA
CCCCGATCATTACCTTCATGAACAAGCTGGACCGCGAGGGGCAGGAACCGCTCGACCTGCTGGACGACATCGAAAAAAAC
CTAAAGATGCAGACCGCGCCCGTAACCTGGCCGGTGGGGATGGGAAAACGCTTTCGCGGCACCTATCACCTCTACACAAA
AGAGCTGATCTTCTTCGATGCCGAGGCTGCCAACGGAACCGGCCGCATCCTGACGATTGCCGGGCTGAATGACCCTCTGC
TGGACGAGCTTCTGGGGAGCCAGGTGGACGAACTACGCCACGACGTGGAGTTGCTGGAAGGGGCCGCCAACCCCTTCGAC
AAGGAGGCCTATCTTGCCGGCCAGCAGACACCGGTCTTTTTCGGCAGCGCCATCAACACCTTTGGCGTCCAGCAACTCCT
GGACGCATTCATCGAATATGCTCCGGCCCCGCTGCCGCGCGAAACTACCACCAGAGTGGTTTCACCCTATGAGGAGCCGT
TCAGCGGCTTCACCTTCAAGATCCAGGCAAATATGGATCCCGCCCACCGGGACCGCATCGCCTTCTTCCGCATCTGCTCC
GGTAAATTCACCCGCGGCATGAAGGTGCGCCACACCCGCCTGGGGCGCGAGGTACAGATCGCCAATGCCACCATCTTCAT
GGCCCAGGATCGCACCAACGTGGAGGAGGCCTGGCCCGGCGACATCATCGGTATCCACAATCACGGAACCATCAAGATCG
GCGACACATTCACCCAAGGCGAGGAGCTGAAATTCACCGGCATCCCCAGCTTCGCCCCTGAGCACTTCCGCAAGGTCCGC
CTGCTCAACCCGCTAAAATCAAAGGCTTTGGAAAAGGGGTTAATACAGCTGGCCGAAGAGGGCGCCACCCAGGTGTTCAA
GCCCCTGATGGGAGCCGACTGGGTGATCGGCGCCGTCGGCCTGCTGCAGTTCGAGGTTGTCATGCACCGTCTGGAGTTTG
AATACAGCGTCAAGGTAGCCTTTGAACCGGTCAGCTACGTCACCGCACGCTGGGTGACAGGCGAGAGAAAAAAAGTCGAG
GAATTCGAGAAGAGGGAAGCCATGCACGTCTACATCGACGGCGAGGGCAAGCTGACCTACATGGCAGGCAGCCAGTGGCG
GCTGGACAATACCATTGAATCATGGAAAGAGCTATCATTCCACGAAACCAGCGAGCACAGCTGA

Upstream 100 bases:

>100_bases
GCAACCACGGAATCGGCCGAAGTCAGCTGCGCGCGCACATTCATACCCTAGAGACCAAGGCGGGCATCCCCCGCCTTTCG
ATTGCATAAGGAGTTTTATC

Downstream 100 bases:

>100_bases
TGACCACGGCAGCAGCCCACCCCAGGCGAGGAAAAGAACATCGCACGGCATCTGTCAGGGCAACCTGGGAGGCTTTCCGC
GGCATCAGGTTCCATCATTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MQNHNRQEVDKRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKSARHATSDWMELEKQRGISVTSSVMKFTYNG
CEINLLDTPGHNDFSEDTYRVLTAVDSVLMVIDSVKGVESQTKKLLEVCRLRHTPIITFMNKLDREGQEPLDLLDDIEKN
LKMQTAPVTWPVGMGKRFRGTYHLYTKELIFFDAEAANGTGRILTIAGLNDPLLDELLGSQVDELRHDVELLEGAANPFD
KEAYLAGQQTPVFFGSAINTFGVQQLLDAFIEYAPAPLPRETTTRVVSPYEEPFSGFTFKIQANMDPAHRDRIAFFRICS
GKFTRGMKVRHTRLGREVQIANATIFMAQDRTNVEEAWPGDIIGIHNHGTIKIGDTFTQGEELKFTGIPSFAPEHFRKVR
LLNPLKSKALEKGLIQLAEEGATQVFKPLMGADWVIGAVGLLQFEVVMHRLEFEYSVKVAFEPVSYVTARWVTGERKKVE
EFEKREAMHVYIDGEGKLTYMAGSQWRLDNTIESWKELSFHETSEHS

Sequences:

>Translated_527_residues
MQNHNRQEVDKRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKSARHATSDWMELEKQRGISVTSSVMKFTYNG
CEINLLDTPGHNDFSEDTYRVLTAVDSVLMVIDSVKGVESQTKKLLEVCRLRHTPIITFMNKLDREGQEPLDLLDDIEKN
LKMQTAPVTWPVGMGKRFRGTYHLYTKELIFFDAEAANGTGRILTIAGLNDPLLDELLGSQVDELRHDVELLEGAANPFD
KEAYLAGQQTPVFFGSAINTFGVQQLLDAFIEYAPAPLPRETTTRVVSPYEEPFSGFTFKIQANMDPAHRDRIAFFRICS
GKFTRGMKVRHTRLGREVQIANATIFMAQDRTNVEEAWPGDIIGIHNHGTIKIGDTFTQGEELKFTGIPSFAPEHFRKVR
LLNPLKSKALEKGLIQLAEEGATQVFKPLMGADWVIGAVGLLQFEVVMHRLEFEYSVKVAFEPVSYVTARWVTGERKKVE
EFEKREAMHVYIDGEGKLTYMAGSQWRLDNTIESWKELSFHETSEHS
>Mature_527_residues
MQNHNRQEVDKRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKSARHATSDWMELEKQRGISVTSSVMKFTYNG
CEINLLDTPGHNDFSEDTYRVLTAVDSVLMVIDSVKGVESQTKKLLEVCRLRHTPIITFMNKLDREGQEPLDLLDDIEKN
LKMQTAPVTWPVGMGKRFRGTYHLYTKELIFFDAEAANGTGRILTIAGLNDPLLDELLGSQVDELRHDVELLEGAANPFD
KEAYLAGQQTPVFFGSAINTFGVQQLLDAFIEYAPAPLPRETTTRVVSPYEEPFSGFTFKIQANMDPAHRDRIAFFRICS
GKFTRGMKVRHTRLGREVQIANATIFMAQDRTNVEEAWPGDIIGIHNHGTIKIGDTFTQGEELKFTGIPSFAPEHFRKVR
LLNPLKSKALEKGLIQLAEEGATQVFKPLMGADWVIGAVGLLQFEVVMHRLEFEYSVKVAFEPVSYVTARWVTGERKKVE
EFEKREAMHVYIDGEGKLTYMAGSQWRLDNTIESWKELSFHETSEHS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=500, Percent_Identity=29.2, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI19923640, Length=508, Percent_Identity=25.9842519685039, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.6649746192893, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI25306287, Length=310, Percent_Identity=30.9677419354839, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=162, Percent_Identity=32.7160493827161, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=33.5766423357664, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI4503483, Length=161, Percent_Identity=31.6770186335404, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=58.2375478927203, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=505, Percent_Identity=27.3267326732673, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI48994988, Length=179, Percent_Identity=34.0782122905028, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1788922, Length=151, Percent_Identity=29.1390728476821, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=501, Percent_Identity=27.3453093812375, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17556745, Length=168, Percent_Identity=33.9285714285714, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=153, Percent_Identity=31.3725490196078, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=36.1290322580645, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=491, Percent_Identity=26.6802443991853, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=420, Percent_Identity=28.0952380952381, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324707, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=25.4777070063694, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582462, Length=492, Percent_Identity=27.0325203252033, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI221458488, Length=515, Percent_Identity=26.2135922330097, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.7044025157233, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585709, Length=163, Percent_Identity=30.0613496932515, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=30.0653594771242, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=30.0653594771242, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_PELPD (A1AME1)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900569.1
- ProteinModelPortal:   A1AME1
- SMR:   A1AME1
- STRING:   A1AME1
- GeneID:   4571777
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0882
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   HERTVEP
- PhylomeDB:   A1AME1
- ProtClustDB:   PRK00741
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59590; Mature: 59590

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNHNRQEVDKRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKSARHATSDWME
CCCCCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKQRGISVTSSVMKFTYNGCEINLLDTPGHNDFSEDTYRVLTAVDSVLMVIDSVKGVES
HHHHCCCHHHHHHHHEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
QTKKLLEVCRLRHTPIITFMNKLDREGQEPLDLLDDIEKNLKMQTAPVTWPVGMGKRFRG
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCCC
TYHLYTKELIFFDAEAANGTGRILTIAGLNDPLLDELLGSQVDELRHDVELLEGAANPFD
EEEEEEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KEAYLAGQQTPVFFGSAINTFGVQQLLDAFIEYAPAPLPRETTTRVVSPYEEPFSGFTFK
CCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEE
IQANMDPAHRDRIAFFRICSGKFTRGMKVRHTRLGREVQIANATIFMAQDRTNVEEAWPG
EECCCCHHHHHHEEEEEEECCCCCCCCCHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCC
DIIGIHNHGTIKIGDTFTQGEELKFTGIPSFAPEHFRKVRLLNPLKSKALEKGLIQLAEE
CEEEECCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GATQVFKPLMGADWVIGAVGLLQFEVVMHRLEFEYSVKVAFEPVSYVTARWVTGERKKVE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHCCCCCHHHHH
EFEKREAMHVYIDGEGKLTYMAGSQWRLDNTIESWKELSFHETSEHS
HHHCCCEEEEEECCCCEEEEEECCCEECHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQNHNRQEVDKRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKSARHATSDWME
CCCCCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKQRGISVTSSVMKFTYNGCEINLLDTPGHNDFSEDTYRVLTAVDSVLMVIDSVKGVES
HHHHCCCHHHHHHHHEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
QTKKLLEVCRLRHTPIITFMNKLDREGQEPLDLLDDIEKNLKMQTAPVTWPVGMGKRFRG
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCCC
TYHLYTKELIFFDAEAANGTGRILTIAGLNDPLLDELLGSQVDELRHDVELLEGAANPFD
EEEEEEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KEAYLAGQQTPVFFGSAINTFGVQQLLDAFIEYAPAPLPRETTTRVVSPYEEPFSGFTFK
CCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEE
IQANMDPAHRDRIAFFRICSGKFTRGMKVRHTRLGREVQIANATIFMAQDRTNVEEAWPG
EECCCCHHHHHHEEEEEEECCCCCCCCCHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCC
DIIGIHNHGTIKIGDTFTQGEELKFTGIPSFAPEHFRKVRLLNPLKSKALEKGLIQLAEE
CEEEECCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GATQVFKPLMGADWVIGAVGLLQFEVVMHRLEFEYSVKVAFEPVSYVTARWVTGERKKVE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHCCCCCHHHHH
EFEKREAMHVYIDGEGKLTYMAGSQWRLDNTIESWKELSFHETSEHS
HHHCCCEEEEEECCCCEEEEEECCCEECHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA