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The map label for this gene is trmJ [H]

Identifier: 118579084

GI number: 118579084

Start: 683174

End: 683929

Strand: Reverse

Name: trmJ [H]

Synonym: Ppro_0645

Alternate gene names: 118579084

Gene position: 683929-683174 (Counterclockwise)

Preceding gene: 118579086

Following gene: 118579083

Centisome position: 17.06

GC content: 62.57

Gene sequence:

>756_bases
ATGAACGAACCAGACAACAGCATCGCGGACAGTGTTTCCATCGTCATGGTGGAACCGCAATCACCCGGCAACATCGGCAT
GGTCTGTCGCGCCATGAAGAACATGGGGCTCTCCCGCCTGCGCCTGGTGAAAGGGTGCGACCGATTTCACCCCGAATCAC
TCAAGTTCGCGGTCTCGGCCAAGGATCTGCTGGAGCGGGCGGAGTTGTACGACGACCTGGCATCCGCCCTGGCAGACTGC
GCCCTGACCGTGGGAACCACCCGCCGCCACGGCAAGTACCGCCAGGAGATACTCTCCCCTCCCCAGGTGGCCACCCTACT
CAGGACGCGGGCACAGGGCGACTGCAGGGGAGCTCTCGTCTTCGGCCGCGAGGACAGCGGCCTGACCACCGATGAACTAT
CCCTCTGCCGCTGGCACGCGACCATCCCCACCTCCAGCGAGTATGGCTCCCTCAACCTGTCCCAGGCGGTCCTACTGTTC
TGCTATGAGTTGGGCAAGGCCAGTGACTCTCCCGGTGGGGGCAGAACGGAAATACCGGCCACCTCGGCCGAAATGGAGAC
GCTCTTCAGCCAGATGGATACCAGCTTGCAGCGCATCGGCTTTCTCAACGAACAGAACCCCGGCCACATCATGCGTTCCC
TGCGCAGGATTCTCTTTCGTTCCGAACTGGATAACCGCGAACTGGCCATCCTGCGAGGCATGTTCTCCCAGGTGGACTGG
GCCTGCTCCGGCTTCGACGGCAGGAAGCGCCTGTGA

Upstream 100 bases:

>100_bases
TTTCATTATCCACTTTACGACAACCAGTCATGAAACCTGCCCGAATACAGCTTCGCCTGAACTCGCTGAAGCAGGGCCGT
ACGGAGCATGAGTACAGAAA

Downstream 100 bases:

>100_bases
ACCCAACCAACGTCGGTCTTGTGGCAGGCACCCTCACCAGCATTGCCGCCCTTCCCCAGGTGATCAAGACCCTGCGAACC
CGCCATACCAGGGACATCTC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNEPDNSIADSVSIVMVEPQSPGNIGMVCRAMKNMGLSRLRLVKGCDRFHPESLKFAVSAKDLLERAELYDDLASALADC
ALTVGTTRRHGKYRQEILSPPQVATLLRTRAQGDCRGALVFGREDSGLTTDELSLCRWHATIPTSSEYGSLNLSQAVLLF
CYELGKASDSPGGGRTEIPATSAEMETLFSQMDTSLQRIGFLNEQNPGHIMRSLRRILFRSELDNRELAILRGMFSQVDW
ACSGFDGRKRL

Sequences:

>Translated_251_residues
MNEPDNSIADSVSIVMVEPQSPGNIGMVCRAMKNMGLSRLRLVKGCDRFHPESLKFAVSAKDLLERAELYDDLASALADC
ALTVGTTRRHGKYRQEILSPPQVATLLRTRAQGDCRGALVFGREDSGLTTDELSLCRWHATIPTSSEYGSLNLSQAVLLF
CYELGKASDSPGGGRTEIPATSAEMETLFSQMDTSLQRIGFLNEQNPGHIMRSLRRILFRSELDNRELAILRGMFSQVDW
ACSGFDGRKRL
>Mature_251_residues
MNEPDNSIADSVSIVMVEPQSPGNIGMVCRAMKNMGLSRLRLVKGCDRFHPESLKFAVSAKDLLERAELYDDLASALADC
ALTVGTTRRHGKYRQEILSPPQVATLLRTRAQGDCRGALVFGREDSGLTTDELSLCRWHATIPTSSEYGSLNLSQAVLLF
CYELGKASDSPGGGRTEIPATSAEMETLFSQMDTSLQRIGFLNEQNPGHIMRSLRRILFRSELDNRELAILRGMFSQVDW
ACSGFDGRKRL

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=242, Percent_Identity=35.1239669421488, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790865, Length=220, Percent_Identity=31.8181818181818, Blast_Score=117, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27764; Mature: 27764

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPDNSIADSVSIVMVEPQSPGNIGMVCRAMKNMGLSRLRLVKGCDRFHPESLKFAVSA
CCCCCCCHHCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEECH
KDLLERAELYDDLASALADCALTVGTTRRHGKYRQEILSPPQVATLLRTRAQGDCRGALV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEE
FGREDSGLTTDELSLCRWHATIPTSSEYGSLNLSQAVLLFCYELGKASDSPGGGRTEIPA
ECCCCCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TSAEMETLFSQMDTSLQRIGFLNEQNPGHIMRSLRRILFRSELDNRELAILRGMFSQVDW
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ACSGFDGRKRL
HHCCCCCCCCC
>Mature Secondary Structure
MNEPDNSIADSVSIVMVEPQSPGNIGMVCRAMKNMGLSRLRLVKGCDRFHPESLKFAVSA
CCCCCCCHHCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHEEEECH
KDLLERAELYDDLASALADCALTVGTTRRHGKYRQEILSPPQVATLLRTRAQGDCRGALV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEE
FGREDSGLTTDELSLCRWHATIPTSSEYGSLNLSQAVLLFCYELGKASDSPGGGRTEIPA
ECCCCCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TSAEMETLFSQMDTSLQRIGFLNEQNPGHIMRSLRRILFRSELDNRELAILRGMFSQVDW
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ACSGFDGRKRL
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA