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The map label for this gene is cspB [H]

Identifier: 118578996

GI number: 118578996

Start: 594617

End: 594820

Strand: Reverse

Name: cspB [H]

Synonym: Ppro_0557

Alternate gene names: 118578996

Gene position: 594820-594617 (Counterclockwise)

Preceding gene: 118578997

Following gene: 118578994

Centisome position: 14.84

GC content: 54.9

Gene sequence:

>204_bases
ATGGCAAAGGGCAAGGTTAAGTGGTTCAACGACACGAAGGGTTTCGGTTTTATCGAGCAGGAAGGCGGCGAGGATGTCTT
CGTGCATTTCTCCGCAATCCAGGGCGAAGGCTTCAAATCCCTCTCCGAGGGTGACGAAGTGACCTTCGACATTACCCAGG
GCCCCAAAGGCCTGCAGTCCGCCAATGTGGTGAAGGTTCGCTAA

Upstream 100 bases:

>100_bases
TTATTCGGCTTTGCGGTTTTCCTGCACCAGACTCCGGAAACTTTGGCGGTAGTACCACTCATTGTTCCGGCATAGCACCG
GAGAAAAAAGGAGGTAGGAA

Downstream 100 bases:

>100_bases
TTCCTTCGTTTTCAACTGAGAATAAAAAAGCCCGCTGAGCAATCAGCGGGCTTTTTTTGTGCCTTTTCCTCGCCCAGCTT
TTCTCTCCCTGCCGGGATGA

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MAKGKVKWFNDTKGFGFIEQEGGEDVFVHFSAIQGEGFKSLSEGDEVTFDITQGPKGLQSANVVKVR

Sequences:

>Translated_67_residues
MAKGKVKWFNDTKGFGFIEQEGGEDVFVHFSAIQGEGFKSLSEGDEVTFDITQGPKGLQSANVVKVR
>Mature_66_residues
AKGKVKWFNDTKGFGFIEQEGGEDVFVHFSAIQGEGFKSLSEGDEVTFDITQGPKGLQSANVVKVR

Specific function: Affects cell viability at low temperatures [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=63.0769230769231, Blast_Score=83, Evalue=4e-18,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=55.2238805970149, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=60, Blast_Score=78, Evalue=8e-17,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=58.0645161290323, Blast_Score=72, Evalue=5e-15,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=57.8125, Blast_Score=72, Evalue=6e-15,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=57.8125, Blast_Score=72, Evalue=7e-15,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=54.6875, Blast_Score=69, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=49.1803278688525, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7324; Mature: 7193

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKVKWFNDTKGFGFIEQEGGEDVFVHFSAIQGEGFKSLSEGDEVTFDITQGPKGLQS
CCCCCEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCC
ANVVKVR
CCEEEEC
>Mature Secondary Structure 
AKGKVKWFNDTKGFGFIEQEGGEDVFVHFSAIQGEGFKSLSEGDEVTFDITQGPKGLQS
CCCCEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCC
ANVVKVR
CCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8294017; 10736231 [H]