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The map label for this gene is kinA [H]

Identifier: 118578862

GI number: 118578862

Start: 440204

End: 441580

Strand: Reverse

Name: kinA [H]

Synonym: Ppro_0420

Alternate gene names: 118578862

Gene position: 441580-440204 (Counterclockwise)

Preceding gene: 118578863

Following gene: 118578851

Centisome position: 11.02

GC content: 56.43

Gene sequence:

>1377_bases
ATGGAAAACCAGAACAACACCCCCCACTGCGACACCACGGACAAGAAACTCAACGAGCTGGCACTGTTGTACCAGCTCAG
CAACACCTTGTTATCAACCATCCGCCTCAACAAACTCATCCACCTGATCCTTACCGCTCTGACATCCGGCCCATCTCCGC
TCTTCCAGCGAGCCATGCTCTTCCTGCACAACGAAAAGTCCCGTACGTTGCAGGGGATGCTCGGCGTCACCAGCGACACC
TCCGAAAGACTGGTTGTCGAGGGCGACGAAGAAAACCGGCTCAGCAACCGCTGGGACATCAGCGATCAGGTCATCGCCCA
CCAACGTGCAACGAACTTTTGCAACCAGGTACGGTCAACCCGCATCGATATCGACGGAAGCTGTCAAGTCATCAGCAAGG
TCATGGTGGAAAACGTCGTATACCATGCCGAGCAACCGGAGAGCGACGCCTGCCCGGCATGCCTTGCCCTCAGGGAGTTG
GGCGTGTACGCCTTTGCCGCCGTCCCCCTGATGGCGCGGGACAAAAACCTGGGCATGATCGCAGTGGACAACCCCGGATC
CGGCGCCAAGATCACCCTGGATGACCTGCACTTCCTGCAACTCTTCGCAAATCAGGCCGGCATGGCCGTGGAAAACTCCA
TGCTCTACAATCGTGTCGAAGAGGCCCACTCCAACCTGCGTGACGCCCGTGAACGCCTGATACGCGGCGAGCGCCTGGCT
GCAGTGGGAGAGATGGCGGCAAAACTGGCCCATGAGCTGAAGAATCCGCTCATAACCATTGGAGGATTCTCCTGGCGCCT
GATCAAGAACCTGGCAACGGAATCGCCCGAATACCGCTACGCCGATATCATCAGCAAAGAGGTCTGTCGCCTGGAGAGGA
TGTTAACCGACATCCTCAGCTTTACCAGCAAACCGATGGTCTGCTTCGACTACGGCAACTGCGATCTGGAGGAGATTCTT
GGCGAGTGTTTCGAAGTCAGCAGCACCCCCCTGGAAGAGAATAACGTAAGCCTGACGACCTCGTTCAGCGGCGGCCCTTG
GATCGTCCTGGGAGACGCCCACCAACTCAAGCAGGTCTTCCTCAACCTGATTCTGAATGCCTGTGAATGCATGACGGACG
GAGGAGCGCTGGAGATCGGGCTACAGAAAGTATTTCTTGACAAAAATTCAGTAATGATTAGCATTAGCGACACTGGCGGC
GGCATCCATCCCGACATGCTGCCCCGGATTTTCGATCCGTTCTTTACCACCAAACCCCAAGGAAGCGGGCTCGGACTGGC
CATTGTCAAGCGCATCATGCTGAACCACAACGGCTCCATTGACGTGGAAAACGGAGAACGAGGAGCGGTTTTCACGTTAA
CACTGCCGCTCATATAG

Upstream 100 bases:

>100_bases
CCCAGTCACGACGCTCTGCATCAACTGCAAGCTGGAAGCCGAGAAGAAGGAAAAACGACCGTAACCCTTAAAATCCTCTT
GCCTCTGTATCGGATAGCGC

Downstream 100 bases:

>100_bases
CATTACGACAAGCATATACGGGGATGTGGCTCAGCTGGGAGAGCACTGCCTTCGCAAGGCAGGGGTCGAGGGTTCGAATC
CCTTCATCTCCACCAACAAA

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MENQNNTPHCDTTDKKLNELALLYQLSNTLLSTIRLNKLIHLILTALTSGPSPLFQRAMLFLHNEKSRTLQGMLGVTSDT
SERLVVEGDEENRLSNRWDISDQVIAHQRATNFCNQVRSTRIDIDGSCQVISKVMVENVVYHAEQPESDACPACLALREL
GVYAFAAVPLMARDKNLGMIAVDNPGSGAKITLDDLHFLQLFANQAGMAVENSMLYNRVEEAHSNLRDARERLIRGERLA
AVGEMAAKLAHELKNPLITIGGFSWRLIKNLATESPEYRYADIISKEVCRLERMLTDILSFTSKPMVCFDYGNCDLEEIL
GECFEVSSTPLEENNVSLTTSFSGGPWIVLGDAHQLKQVFLNLILNACECMTDGGALEIGLQKVFLDKNSVMISISDTGG
GIHPDMLPRIFDPFFTTKPQGSGLGLAIVKRIMLNHNGSIDVENGERGAVFTLTLPLI

Sequences:

>Translated_458_residues
MENQNNTPHCDTTDKKLNELALLYQLSNTLLSTIRLNKLIHLILTALTSGPSPLFQRAMLFLHNEKSRTLQGMLGVTSDT
SERLVVEGDEENRLSNRWDISDQVIAHQRATNFCNQVRSTRIDIDGSCQVISKVMVENVVYHAEQPESDACPACLALREL
GVYAFAAVPLMARDKNLGMIAVDNPGSGAKITLDDLHFLQLFANQAGMAVENSMLYNRVEEAHSNLRDARERLIRGERLA
AVGEMAAKLAHELKNPLITIGGFSWRLIKNLATESPEYRYADIISKEVCRLERMLTDILSFTSKPMVCFDYGNCDLEEIL
GECFEVSSTPLEENNVSLTTSFSGGPWIVLGDAHQLKQVFLNLILNACECMTDGGALEIGLQKVFLDKNSVMISISDTGG
GIHPDMLPRIFDPFFTTKPQGSGLGLAIVKRIMLNHNGSIDVENGERGAVFTLTLPLI
>Mature_458_residues
MENQNNTPHCDTTDKKLNELALLYQLSNTLLSTIRLNKLIHLILTALTSGPSPLFQRAMLFLHNEKSRTLQGMLGVTSDT
SERLVVEGDEENRLSNRWDISDQVIAHQRATNFCNQVRSTRIDIDGSCQVISKVMVENVVYHAEQPESDACPACLALREL
GVYAFAAVPLMARDKNLGMIAVDNPGSGAKITLDDLHFLQLFANQAGMAVENSMLYNRVEEAHSNLRDARERLIRGERLA
AVGEMAAKLAHELKNPLITIGGFSWRLIKNLATESPEYRYADIISKEVCRLERMLTDILSFTSKPMVCFDYGNCDLEEIL
GECFEVSSTPLEENNVSLTTSFSGGPWIVLGDAHQLKQVFLNLILNACECMTDGGALEIGLQKVFLDKNSVMISISDTGG
GIHPDMLPRIFDPFFTTKPQGSGLGLAIVKRIMLNHNGSIDVENGERGAVFTLTLPLI

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=31.2757201646091, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=31.8965517241379, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786783, Length=223, Percent_Identity=28.6995515695067, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=26.9406392694064, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=319, Percent_Identity=28.5266457680251, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=30.0884955752212, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=25.1046025104602, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994928, Length=262, Percent_Identity=24.8091603053435, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789149, Length=273, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=22.9357798165138, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=28.3842794759825, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=28.448275862069, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789808, Length=214, Percent_Identity=27.1028037383178, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50657; Mature: 50657

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQNNTPHCDTTDKKLNELALLYQLSNTLLSTIRLNKLIHLILTALTSGPSPLFQRAML
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FLHNEKSRTLQGMLGVTSDTSERLVVEGDEENRLSNRWDISDQVIAHQRATNFCNQVRST
HHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
RIDIDGSCQVISKVMVENVVYHAEQPESDACPACLALRELGVYAFAAVPLMARDKNLGMI
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
AVDNPGSGAKITLDDLHFLQLFANQAGMAVENSMLYNRVEEAHSNLRDARERLIRGERLA
EECCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AVGEMAAKLAHELKNPLITIGGFSWRLIKNLATESPEYRYADIISKEVCRLERMLTDILS
HHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FTSKPMVCFDYGNCDLEEILGECFEVSSTPLEENNVSLTTSFSGGPWIVLGDAHQLKQVF
HCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHH
LNLILNACECMTDGGALEIGLQKVFLDKNSVMISISDTGGGIHPDMLPRIFDPFFTTKPQ
HHHHHHHHHHHCCCCEEEECCEEEEECCCCEEEEEECCCCCCCHHHHHHHHCHHHCCCCC
GSGLGLAIVKRIMLNHNGSIDVENGERGAVFTLTLPLI
CCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCC
>Mature Secondary Structure
MENQNNTPHCDTTDKKLNELALLYQLSNTLLSTIRLNKLIHLILTALTSGPSPLFQRAML
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FLHNEKSRTLQGMLGVTSDTSERLVVEGDEENRLSNRWDISDQVIAHQRATNFCNQVRST
HHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
RIDIDGSCQVISKVMVENVVYHAEQPESDACPACLALRELGVYAFAAVPLMARDKNLGMI
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
AVDNPGSGAKITLDDLHFLQLFANQAGMAVENSMLYNRVEEAHSNLRDARERLIRGERLA
EECCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AVGEMAAKLAHELKNPLITIGGFSWRLIKNLATESPEYRYADIISKEVCRLERMLTDILS
HHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FTSKPMVCFDYGNCDLEEILGECFEVSSTPLEENNVSLTTSFSGGPWIVLGDAHQLKQVF
HCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHH
LNLILNACECMTDGGALEIGLQKVFLDKNSVMISISDTGGGIHPDMLPRIFDPFFTTKPQ
HHHHHHHHHHHCCCCEEEECCEEEEECCCCEEEEEECCCCCCCHHHHHHHHCHHHCCCCC
GSGLGLAIVKRIMLNHNGSIDVENGERGAVFTLTLPLI
CCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]