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The map label for this gene is dnaK [H]

Identifier: 118578601

GI number: 118578601

Start: 162174

End: 163775

Strand: Reverse

Name: dnaK [H]

Synonym: Ppro_0157

Alternate gene names: 118578601

Gene position: 163775-162174 (Counterclockwise)

Preceding gene: 118578602

Following gene: 118578600

Centisome position: 4.09

GC content: 58.61

Gene sequence:

>1602_bases
ATGTACTACGGCATAGACCTGGGAACGACAACCACCTTGATCGCCGAACCGGACATCAAGGGTTCAAGCTACGCTGCAAG
ACAGCTGAAGATTCCCCAGCAGGACAAGGCTGGAAATTCCATCGAACGAGACGACCTCCCCTCGGTCGCCTACTTTCCCG
GCAGCGAGGAACCGGTGGTCGGAATGAGGGCCAAGGAGGGGGTGTGCGGAGCGGTGGGCAAGGTGGTGTGCGCGGTAAAG
CGCCTGATGGGGCGCGACGTGCAGCCGTTCGTCTTCGACGGCAAGTCCTACGACCCAAGCGATATCTCCTCTCTCTACAT
AGCACACGTCCTTAGCCAGGCGGTGCTGCTGAAAAAAGGGAGAGTATCGAAGCTGACAGTGACTGTCCCCGCATCGTTTA
CCACAAAGCAGCGCAAAGACACCCAGAAGGCGATCCAGAAGGCCTGCGAACGCCTGAAGGTGAAGTACGACGACAATGCC
CTCATCAGCGAACCTCTCGCCGCACTGCTCAGCTACATCTCCTACGAGATAGCCACCTCCACCATCAGGATCGATATCGA
CAAGAAACCGCTTGTCCTGGTTTACGACATAGGGGGCGGGACCCTCGACCTCACGCTGGTCCGACTCCAGTGGAAGGACC
AGCGCGGGGAGAAAGACCTGAAGAACGTCGACTTCCGGGTCCAGAGGGTGGGGCGCTACAATCAGGTGGGAGGCGAGGAT
TTCGACGAGCTGATCGCCGAGTGGTTGCTGGAGACGATGCTAGAGCAGTTCCCCGAGATGGACATACAGCTGGATCGGTA
CGAGCGCAAAGCTGTGAAAGCGCAGCTGATGGCGATAGCCGAGCAGCGGAAGATCGAATTCAACAAGGAGTACTGCAGCG
ACTGGGACCATGAGCCGGTGGTCAACACGGTCGAGGTACGGCAGGTCCGGTACGAGGTGGAAAGCACCCTGACGCATTCC
GACGTGGTGAGAGTGCTGGAGCCGTTGATCGGGGAGGCGGACACGCCGAAGAACTGTGTGACTAGCATCCAGAAGTTCCT
CGCCAAGCAGGGGGTCACCATGGCACAGGTGGACCACGTCCTCTGCGTCGGGGGGATGTCGAGGTTCATGCCGCTCAAGG
AGGCGCTGGAGAAGAAGTACACCCCTGCCGGAGAAAAGTCGAAGTTCATCTTCAACGACTCCCTGGCGGACGTTGCCGTT
GCCAAGGGAGCTGCCATCTACAGCTACATGAAGGCTGAGCACACCCCTCCCATCCAGGAGCCTCGCGCCGACGCCTACTA
CGCCAAAACGGAGAAGGGATTCAAGAAGATCCTCGGCTGCATCGAGTCTGAAAACGCCACTTTGTTCACAACGGTAGAAG
CGGGAGATTACCTCGACCTCTACGTCTTCGCCGGCGAGGACTGCGCCGCCGAGTACGCCGAGCCGCGGGTCCTGACCTCC
CTCATGTACCAGGGGGGGGAGAGGATTCGTTTTCCCCATACCTTTCCCAAGGGAACCGCACTGGAAGTAAAGATGGCGGT
CGAAAACGACAACAAGGTCCCTCGCTATGAAATAAAGGCGGATGGCGTCGCGATTCATACCAACGTCATGGAGATACCGT
AG

Upstream 100 bases:

>100_bases
GGAACCCTGCTTCGCCTAAACACGATTCGGAAACTACTGCGGTGGGAAAAGCCTTCCCAACCCGTCGCGCTTCGCTGAAA
CCGCACTACGAGAGGTTCGA

Downstream 100 bases:

>100_bases
CATGAGAAATGTTTTTGCACCGGATGCCACCTATTTTTCGGACCTATCTGCCGACCCTAACGTCGCTATACACGTTCAGC
AGCTGATCCAGGAATACCAG

Product: heat shock protein 70

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MYYGIDLGTTTTLIAEPDIKGSSYAARQLKIPQQDKAGNSIERDDLPSVAYFPGSEEPVVGMRAKEGVCGAVGKVVCAVK
RLMGRDVQPFVFDGKSYDPSDISSLYIAHVLSQAVLLKKGRVSKLTVTVPASFTTKQRKDTQKAIQKACERLKVKYDDNA
LISEPLAALLSYISYEIATSTIRIDIDKKPLVLVYDIGGGTLDLTLVRLQWKDQRGEKDLKNVDFRVQRVGRYNQVGGED
FDELIAEWLLETMLEQFPEMDIQLDRYERKAVKAQLMAIAEQRKIEFNKEYCSDWDHEPVVNTVEVRQVRYEVESTLTHS
DVVRVLEPLIGEADTPKNCVTSIQKFLAKQGVTMAQVDHVLCVGGMSRFMPLKEALEKKYTPAGEKSKFIFNDSLADVAV
AKGAAIYSYMKAEHTPPIQEPRADAYYAKTEKGFKKILGCIESENATLFTTVEAGDYLDLYVFAGEDCAAEYAEPRVLTS
LMYQGGERIRFPHTFPKGTALEVKMAVENDNKVPRYEIKADGVAIHTNVMEIP

Sequences:

>Translated_533_residues
MYYGIDLGTTTTLIAEPDIKGSSYAARQLKIPQQDKAGNSIERDDLPSVAYFPGSEEPVVGMRAKEGVCGAVGKVVCAVK
RLMGRDVQPFVFDGKSYDPSDISSLYIAHVLSQAVLLKKGRVSKLTVTVPASFTTKQRKDTQKAIQKACERLKVKYDDNA
LISEPLAALLSYISYEIATSTIRIDIDKKPLVLVYDIGGGTLDLTLVRLQWKDQRGEKDLKNVDFRVQRVGRYNQVGGED
FDELIAEWLLETMLEQFPEMDIQLDRYERKAVKAQLMAIAEQRKIEFNKEYCSDWDHEPVVNTVEVRQVRYEVESTLTHS
DVVRVLEPLIGEADTPKNCVTSIQKFLAKQGVTMAQVDHVLCVGGMSRFMPLKEALEKKYTPAGEKSKFIFNDSLADVAV
AKGAAIYSYMKAEHTPPIQEPRADAYYAKTEKGFKKILGCIESENATLFTTVEAGDYLDLYVFAGEDCAAEYAEPRVLTS
LMYQGGERIRFPHTFPKGTALEVKMAVENDNKVPRYEIKADGVAIHTNVMEIP
>Mature_533_residues
MYYGIDLGTTTTLIAEPDIKGSSYAARQLKIPQQDKAGNSIERDDLPSVAYFPGSEEPVVGMRAKEGVCGAVGKVVCAVK
RLMGRDVQPFVFDGKSYDPSDISSLYIAHVLSQAVLLKKGRVSKLTVTVPASFTTKQRKDTQKAIQKACERLKVKYDDNA
LISEPLAALLSYISYEIATSTIRIDIDKKPLVLVYDIGGGTLDLTLVRLQWKDQRGEKDLKNVDFRVQRVGRYNQVGGED
FDELIAEWLLETMLEQFPEMDIQLDRYERKAVKAQLMAIAEQRKIEFNKEYCSDWDHEPVVNTVEVRQVRYEVESTLTHS
DVVRVLEPLIGEADTPKNCVTSIQKFLAKQGVTMAQVDHVLCVGGMSRFMPLKEALEKKYTPAGEKSKFIFNDSLADVAV
AKGAAIYSYMKAEHTPPIQEPRADAYYAKTEKGFKKILGCIESENATLFTTVEAGDYLDLYVFAGEDCAAEYAEPRVLTS
LMYQGGERIRFPHTFPKGTALEVKMAVENDNKVPRYEIKADGVAIHTNVMEIP

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI24234688, Length=432, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI16507237, Length=438, Percent_Identity=25.3424657534247, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13676857, Length=442, Percent_Identity=23.9819004524887, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788875, Length=395, Percent_Identity=25.5696202531646, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786196, Length=438, Percent_Identity=26.9406392694064, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564617, Length=399, Percent_Identity=25.31328320802, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562024, Length=431, Percent_Identity=25.754060324826, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17568549, Length=436, Percent_Identity=25.4587155963303, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17534771, Length=436, Percent_Identity=25, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320805, Length=432, Percent_Identity=26.6203703703704, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6322505, Length=430, Percent_Identity=25.8139534883721, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323401, Length=445, Percent_Identity=24.7191011235955, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322426, Length=429, Percent_Identity=26.8065268065268, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319314, Length=437, Percent_Identity=24.9427917620137, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320950, Length=438, Percent_Identity=24.4292237442922, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319396, Length=436, Percent_Identity=24.7706422018349, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319972, Length=437, Percent_Identity=26.7734553775744, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324120, Length=437, Percent_Identity=26.5446224256293, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24653595, Length=428, Percent_Identity=27.5700934579439, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24641406, Length=437, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641408, Length=437, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641402, Length=437, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641404, Length=437, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571678, Length=444, Percent_Identity=24.5495495495495, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646324, Length=444, Percent_Identity=24.5495495495495, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646322, Length=444, Percent_Identity=24.5495495495495, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646318, Length=444, Percent_Identity=24.5495495495495, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646137, Length=444, Percent_Identity=24.3243243243243, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646135, Length=444, Percent_Identity=24.3243243243243, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17738165, Length=430, Percent_Identity=23.953488372093, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17647515, Length=435, Percent_Identity=25.0574712643678, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 59612; Mature: 59612

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGIDLGTTTTLIAEPDIKGSSYAARQLKIPQQDKAGNSIERDDLPSVAYFPGSEEPVV
CEEEEECCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEE
GMRAKEGVCGAVGKVVCAVKRLMGRDVQPFVFDGKSYDPSDISSLYIAHVLSQAVLLKKG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
RVSKLTVTVPASFTTKQRKDTQKAIQKACERLKVKYDDNALISEPLAALLSYISYEIATS
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHEEE
TIRIDIDKKPLVLVYDIGGGTLDLTLVRLQWKDQRGEKDLKNVDFRVQRVGRYNQVGGED
EEEEEECCCCEEEEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCC
FDELIAEWLLETMLEQFPEMDIQLDRYERKAVKAQLMAIAEQRKIEFNKEYCSDWDHEPV
HHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
VNTVEVRQVRYEVESTLTHSDVVRVLEPLIGEADTPKNCVTSIQKFLAKQGVTMAQVDHV
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LCVGGMSRFMPLKEALEKKYTPAGEKSKFIFNDSLADVAVAKGAAIYSYMKAEHTPPIQE
HHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHCCHHHHHHHHHCCCCCCCC
PRADAYYAKTEKGFKKILGCIESENATLFTTVEAGDYLDLYVFAGEDCAAEYAEPRVLTS
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEECCCHHHHHCCHHHHHH
LMYQGGERIRFPHTFPKGTALEVKMAVENDNKVPRYEIKADGVAIHTNVMEIP
HHHCCCCEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEECCEEEEEEEEECC
>Mature Secondary Structure
MYYGIDLGTTTTLIAEPDIKGSSYAARQLKIPQQDKAGNSIERDDLPSVAYFPGSEEPVV
CEEEEECCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEE
GMRAKEGVCGAVGKVVCAVKRLMGRDVQPFVFDGKSYDPSDISSLYIAHVLSQAVLLKKG
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
RVSKLTVTVPASFTTKQRKDTQKAIQKACERLKVKYDDNALISEPLAALLSYISYEIATS
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHEEE
TIRIDIDKKPLVLVYDIGGGTLDLTLVRLQWKDQRGEKDLKNVDFRVQRVGRYNQVGGED
EEEEEECCCCEEEEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCC
FDELIAEWLLETMLEQFPEMDIQLDRYERKAVKAQLMAIAEQRKIEFNKEYCSDWDHEPV
HHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
VNTVEVRQVRYEVESTLTHSDVVRVLEPLIGEADTPKNCVTSIQKFLAKQGVTMAQVDHV
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LCVGGMSRFMPLKEALEKKYTPAGEKSKFIFNDSLADVAVAKGAAIYSYMKAEHTPPIQE
HHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHCCHHHHHHHHHCCCCCCCC
PRADAYYAKTEKGFKKILGCIESENATLFTTVEAGDYLDLYVFAGEDCAAEYAEPRVLTS
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEECCCHHHHHCCHHHHHH
LMYQGGERIRFPHTFPKGTALEVKMAVENDNKVPRYEIKADGVAIHTNVMEIP
HHHCCCCEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEECCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]