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The map label for this gene is glyA

Identifier: 118480352

GI number: 118480352

Start: 5075103

End: 5076347

Strand: Reverse

Name: glyA

Synonym: BALH_4816

Alternate gene names: 118480352

Gene position: 5076347-5075103 (Counterclockwise)

Preceding gene: 118480353

Following gene: 118480351

Centisome position: 96.56

GC content: 40.16

Gene sequence:

>1245_bases
TTGGTGGATCATTTAAAACGTCAAGATGAAAAGGTATTTGCTGCAATTGAGGCAGAACTAGGAAGACAGCGTTCGAAGAT
TGAGTTAATTGCTTCGGAAAACTTCGTAAGTGAAGCGGTAATGGAGGCGCAAGGTTCTGTTTTAACGAATAAGTATGCTG
AAGGATATCCTGGGAAACGTTACTATGGTGGTTGTGAACACGTAGACGTAGTAGAAGATATCGCACGTGATCGCGTAAAA
GAAATTTTCGGCGCAGAGCACGTAAATGTTCAACCGCATTCTGGTGCACAAGCGAACATGGCAGTATACTTCACGATTTT
AGAGCAAGGCGATACAGTACTTGGTATGAATTTATCTCATGGTGGTCACTTAACACACGGAAGCCCTGTTAACTTCAGTG
GAGTACAATATAATTTCGTAGAATATGGCGTGGATGCTGAATCTCACCGTATTAATTACGATGATGTATTAGCAAAAGCG
AAAGAACATAAACCAAAATTAATCGTTGCAGGTGCAAGTGCATACCCTCGTGTTATCGATTTCAAACGATTCCGTGAGAT
TGCAGATGAAGTGGGCGCATACTTAATGGTGGATATGGCACATATCGCTGGTTTAGTAGCCGCTGGTTTACATCCAAACC
CAGTACCACATGCACATTTCGTTACAACGACAACACATAAAACGTTACGTGGCCCACGTGGTGGTATGATTTTATGTGAA
GAACAATTTGCAAAACAAATTGATAAGTCAATCTTCCCTGGTATTCAAGGTGGTCCACTTATGCACGTAATCGCTGCAAA
AGCTGTTGCGTTTGGTGAGGCACTTCAAGATGATTTCAAAACATATGCACAAAATATCATTAACAATGCGAACCGCTTAG
CTGAAGGTCTTCAAAAAGAAGGACTTACACTTGTTTCTGGTGGAACAGACAATCACTTAATCTTAATTGATGTTCGTAAC
TTAGAAATCACAGGTAAAGTAGCAGAGCATGTATTAGATGAAGTTGGTATTACAGTGAACAAAAATACAATTCCATTTGA
AACAGCAAGCCCATTTGTAACAAGTGGTGTACGTATCGGTACAGCAGCTGTAACATCTCGTGGTTTCGGTTTAGAAGACA
TGGATGAAATTGCGTCACTTATTGCTTACACATTAAAAAATCATGAAAATGAAGCTGCATTAGAAGAAGCACGTAAGCGT
GTAGAAGCGTTAACGAGCAAATTCCCAATGTACACAGATCTATAA

Upstream 100 bases:

>100_bases
ATGAAGGTTCGAAAGAATTACAACCTGAATTTTCGTTTTTTCTGAATAAATAAGAAAAAACTGTTGGGAATCGATGTAAT
TCGTTCAGAAAAGGGGGATT

Downstream 100 bases:

>100_bases
TAGATTGAAGAAGACTGCTGAAACGCAATTGTTTTGGCAGTCTTTTTTGTGGACATATATTGTTCTTTAAGTATGTATAC
AAATGATGAATAAATTTTGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL

Sequences:

>Translated_414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL
>Mature_414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=41.0596026490066, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=41.0596026490066, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=41.0596026490066, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=41.0596026490066, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI261862346, Length=448, Percent_Identity=41.0714285714286, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI22547186, Length=457, Percent_Identity=41.7943107221007, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI22547189, Length=438, Percent_Identity=41.324200913242, Blast_Score=295, Evalue=5e-80,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=57.7937649880096, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144732, Length=453, Percent_Identity=42.6048565121413, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=453, Percent_Identity=42.6048565121413, Blast_Score=355, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=415, Percent_Identity=46.0240963855422, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=405, Percent_Identity=43.7037037037037, Blast_Score=321, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24640005, Length=452, Percent_Identity=42.0353982300885, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=452, Percent_Identity=42.0353982300885, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_BACAH (A0RLA3)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_897503.1
- ProteinModelPortal:   A0RLA3
- SMR:   A0RLA3
- STRING:   A0RLA3
- EnsemblBacteria:   EBBACT00000067318
- GeneID:   4543377
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_4816
- eggNOG:   COG0112
- GeneTree:   EBGT00050000001967
- HOGENOM:   HBG301263
- OMA:   GRAYVTC
- ProtClustDB:   PRK00011
- BioCyc:   BTHU412694:BALH_4816-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45264; Mature: 45264

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCC
YYGGCEHVDVVEDIARDRVKEIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECC
GGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKAKEHKPKLIVAGASAYPRVID
CCCCCCCCCCCCCCEEEEHEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHH
FKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEEEH
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIG
CCEEEECCCCCEEEEEEECCEEECHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCEEEC
TAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKRVEALTSKFPMYTDL
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCC
YYGGCEHVDVVEDIARDRVKEIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECC
GGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKAKEHKPKLIVAGASAYPRVID
CCCCCCCCCCCCCCEEEEHEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHH
FKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEEEH
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIG
CCEEEECCCCCEEEEEEECCEEECHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCEEEC
TAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKRVEALTSKFPMYTDL
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA