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The map label for this gene is opuD [H]

Identifier: 118480277

GI number: 118480277

Start: 4990732

End: 4992288

Strand: Reverse

Name: opuD [H]

Synonym: BALH_4737

Alternate gene names: 118480277

Gene position: 4992288-4990732 (Counterclockwise)

Preceding gene: 118480280

Following gene: 118480270

Centisome position: 94.96

GC content: 38.15

Gene sequence:

>1557_bases
ATGTTATTTACAAAAGTGAAACCTACGAAGGGGGATTTGATGAGGAAACTGACAAAAACATTCATCGTTTCATTAACATT
ATGTATTGCATTTACACTTTGGGGGATTATTCCCGAATCTATTATTGGAAAAGGTAGCCTAGGAAATGTAACAACCGCAA
TTCAAACTGCATTAGTTAGTAAGTTTGGATGGTTCTATATTATTTCTGTTTCTATTATTTTAGGTGTGTCTATCTTTTTA
ATTGTTTCGAAATACGGTTCTATTCGTTTAGGTAAAGATGATGACGAGCCTGATTATAGTTATATGACATGGTTTGCTAT
GTTATTTAGTGCTGGTATGGGTATCGGCTTAGTCTTCTGGGGCGTTGCGGAACCATTAAACCATTTGTATGCACCTCCGT
TTGGAGAGAGTGCAACTGAGGAAAGTGCACGTCTTGCACTGCGTTTTTCATTTTTCCATTGGGGATTACATCCTTGGGGA
CTATATGCATTTGTAGCGCTTTGTATTGCTTACTTTACTTTTAGAAAAGGAAAAGCAAGTACAATTAGTGCGACAGTAGG
GCCGTTATTTAAAGGCGGGGACCATGGACGTATTGCTCATTTATTTGATGTGTTAGCTGTTTTCGCAACTGTGTTTGGCG
TGGCAACATCATTAGGTCTTGGTGCAAAACAAATTGCCGGTGGTGTTAGTTATTTAACATCCATCCCGAATTCATTAACG
ACTCAGTTAGTTATTATTGCAATCGTAACAGTGTTATTTATGTTATCTGCGCAAACAGGTCTTGATAAAGGAATTAAATA
TTTAAGTAATACGAATATTATTTTGGCATTTGCACTTATGATTATTGTATTATTTGCGGGTCCAACAAACTTTATCATGA
ATTACTTCACCTCAACGATTGGTGCTTACATTCAGGAATTGCCAAGCATGAGTTTCCGATTAAGTCCATTAGATGAAGGT
GGAAACCAATGGATTCAATCGTGGACAATTTTCTATTGGGCATGGTGGATTGCATGGTCACCATTCGTAGGTACATTTAT
TGCTCGTGTTTCACGAGGACGTACCATTCGTGAGTTTGTTATCGGTGTGTTACTCGTACCGACCGTAATTGGTGCCCTTT
GGTTCTCTGTTTTCGGCGGAACTGGTATTCATATGGAGCTGTTCGGTGATGCACATATTTTTGAAAAAGTGAAAGAGATG
GGAACAGAAGTAGGGTTATTCGCTATGTTTGATCAGATGGGAAGCTTTGGATCAGCTTTATCTGTTCTAGCTATTCTTCT
TATTTCTACATTCTTTATTACATCTGCAGATTCAGCGACATTCGTTTTAGGAATGTTAACGACACATGGTAGTTTAAATC
CGCCAAACCGCATTAAAATGATCTGGGGTATCGTTTTAGCAGCCCTAGCTTCTATCTTATTATATGTAGGTGGCTTAGAG
GCCTTACAAACGGCAGCTATCATTGCAGCATTCCCATTCGTCTTCGTTATTTTCTTTATGATGGCAGCCTTATTTAAAGA
GTTACAAAAAGAAGGACGTATGAAGCGTCATAAATAA

Upstream 100 bases:

>100_bases
AAGGTAAGGCTGTCCATAAGAATTCGAATTGGTGAAGGTGAAAGTAAGAAAATCACCACAAAAATCATAGTTTTCTATCC
GATTTTACATATCGGAATTT

Downstream 100 bases:

>100_bases
TAGAAGGAAAAAGAGCTGATTATGTATCAGCTCTTTTTTATATAGAAGAAACTTGAACTGCAAGTAAGGGAAATAAAATA
CTTTACTATATTAGTAGTAT

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MLFTKVKPTKGDLMRKLTKTFIVSLTLCIAFTLWGIIPESIIGKGSLGNVTTAIQTALVSKFGWFYIISVSIILGVSIFL
IVSKYGSIRLGKDDDEPDYSYMTWFAMLFSAGMGIGLVFWGVAEPLNHLYAPPFGESATEESARLALRFSFFHWGLHPWG
LYAFVALCIAYFTFRKGKASTISATVGPLFKGGDHGRIAHLFDVLAVFATVFGVATSLGLGAKQIAGGVSYLTSIPNSLT
TQLVIIAIVTVLFMLSAQTGLDKGIKYLSNTNIILAFALMIIVLFAGPTNFIMNYFTSTIGAYIQELPSMSFRLSPLDEG
GNQWIQSWTIFYWAWWIAWSPFVGTFIARVSRGRTIREFVIGVLLVPTVIGALWFSVFGGTGIHMELFGDAHIFEKVKEM
GTEVGLFAMFDQMGSFGSALSVLAILLISTFFITSADSATFVLGMLTTHGSLNPPNRIKMIWGIVLAALASILLYVGGLE
ALQTAAIIAAFPFVFVIFFMMAALFKELQKEGRMKRHK

Sequences:

>Translated_518_residues
MLFTKVKPTKGDLMRKLTKTFIVSLTLCIAFTLWGIIPESIIGKGSLGNVTTAIQTALVSKFGWFYIISVSIILGVSIFL
IVSKYGSIRLGKDDDEPDYSYMTWFAMLFSAGMGIGLVFWGVAEPLNHLYAPPFGESATEESARLALRFSFFHWGLHPWG
LYAFVALCIAYFTFRKGKASTISATVGPLFKGGDHGRIAHLFDVLAVFATVFGVATSLGLGAKQIAGGVSYLTSIPNSLT
TQLVIIAIVTVLFMLSAQTGLDKGIKYLSNTNIILAFALMIIVLFAGPTNFIMNYFTSTIGAYIQELPSMSFRLSPLDEG
GNQWIQSWTIFYWAWWIAWSPFVGTFIARVSRGRTIREFVIGVLLVPTVIGALWFSVFGGTGIHMELFGDAHIFEKVKEM
GTEVGLFAMFDQMGSFGSALSVLAILLISTFFITSADSATFVLGMLTTHGSLNPPNRIKMIWGIVLAALASILLYVGGLE
ALQTAAIIAAFPFVFVIFFMMAALFKELQKEGRMKRHK
>Mature_518_residues
MLFTKVKPTKGDLMRKLTKTFIVSLTLCIAFTLWGIIPESIIGKGSLGNVTTAIQTALVSKFGWFYIISVSIILGVSIFL
IVSKYGSIRLGKDDDEPDYSYMTWFAMLFSAGMGIGLVFWGVAEPLNHLYAPPFGESATEESARLALRFSFFHWGLHPWG
LYAFVALCIAYFTFRKGKASTISATVGPLFKGGDHGRIAHLFDVLAVFATVFGVATSLGLGAKQIAGGVSYLTSIPNSLT
TQLVIIAIVTVLFMLSAQTGLDKGIKYLSNTNIILAFALMIIVLFAGPTNFIMNYFTSTIGAYIQELPSMSFRLSPLDEG
GNQWIQSWTIFYWAWWIAWSPFVGTFIARVSRGRTIREFVIGVLLVPTVIGALWFSVFGGTGIHMELFGDAHIFEKVKEM
GTEVGLFAMFDQMGSFGSALSVLAILLISTFFITSADSATFVLGMLTTHGSLNPPNRIKMIWGIVLAALASILLYVGGLE
ALQTAAIIAAFPFVFVIFFMMAALFKELQKEGRMKRHK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=462, Percent_Identity=37.8787878787879, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788102, Length=449, Percent_Identity=29.6213808463252, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1786224, Length=483, Percent_Identity=29.6066252587992, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56780; Mature: 56780

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLFTKVKPTKGDLMRKLTKTFIVSLTLCIAFTLWGIIPESIIGKGSLGNVTTAIQTALVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KFGWFYIISVSIILGVSIFLIVSKYGSIRLGKDDDEPDYSYMTWFAMLFSAGMGIGLVFW
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
GVAEPLNHLYAPPFGESATEESARLALRFSFFHWGLHPWGLYAFVALCIAYFTFRKGKAS
HHHHHHHHHCCCCCCCCCCHHHHHHHHEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
TISATVGPLFKGGDHGRIAHLFDVLAVFATVFGVATSLGLGAKQIAGGVSYLTSIPNSLT
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
TQLVIIAIVTVLFMLSAQTGLDKGIKYLSNTNIILAFALMIIVLFAGPTNFIMNYFTSTI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GAYIQELPSMSFRLSPLDEGGNQWIQSWTIFYWAWWIAWSPFVGTFIARVSRGRTIREFV
HHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IGVLLVPTVIGALWFSVFGGTGIHMELFGDAHIFEKVKEMGTEVGLFAMFDQMGSFGSAL
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHH
SVLAILLISTFFITSADSATFVLGMLTTHGSLNPPNRIKMIWGIVLAALASILLYVGGLE
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
ALQTAAIIAAFPFVFVIFFMMAALFKELQKEGRMKRHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]