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The map label for this gene is vacB [H]

Identifier: 118480167

GI number: 118480167

Start: 4865452

End: 4867872

Strand: Reverse

Name: vacB [H]

Synonym: BALH_4622

Alternate gene names: 118480167

Gene position: 4867872-4865452 (Counterclockwise)

Preceding gene: 118480168

Following gene: 118480166

Centisome position: 92.6

GC content: 41.06

Gene sequence:

>2421_bases
TTGGAAGAAATCATACAAGAACATATTGATAAGTTGTTATTATTTATGAGAGAAGAAGCGTATAAACCGCTAACGATACA
AGAGTTAGAAGAGGCATTTGGGATTGAAGGTTCTGAGGGCTTTAAAGATTTCGTAAAGGCACTTGTAACGATGGAAGAAA
AGGGACTTGTTATTCGTACTCGTAGCAACCGTTACGGTCTTCCTGAAAAGATGAATTTAGTACGTGGTAAGTTAATTGGA
CATGCACGTGGCTTTGCATTCGTTGTACCAGACGAGAAGAAAACAGGAGATGATGATCTTTTTATCCCGCCTACAGAGTT
AAACGGTGCACTTCATGGCGATACAGTATTAGCACGCCTTAGTTCCCAATCGAGTGGTTCGCGTCAAGAAGGTTCCATTG
TACGCATTTTAGAGCGTGGCACGAAAGAATTAGTTGGTACATATACAGAATCGAAAAACTTTGGATTTGTTATACCTGAC
AATAAACGTTGGACAAGTGACATTTTCGTATTGAAAAGCGCATCAATGGGCGCTGTAGAAGGTCATAAAGTAGTTGTGAA
AATTACGAGCTATCCAGAGAATCGTTTAAGTGCAGAAGGTGAAGTTATTCAAATTCTAGGTCATAAAAATGATCCAGGAG
TAGATATTTTATCTGTCATTCATAAACATCACTTGCCTTTAGCGTTCCCTGAAGAAGTGATGGAACATGCAAACAGTGTA
CCAGAAACGATTTCAGAAGAAGATTTAAAAGATCGCCGTGACCTACGTGATCAAATGATCGTAACAATTGACGGTGCAGA
CGCGAAAGATTTAGATGACGCTGTTACAGTAACGAAGCTCGAGAACGGTAACTATAAGCTTGGCGTTCACATTGCGGATG
TAAGCCACTACGTTCAAGAAGGTTCTCCAATTGATGTAGAAGCAGCGGAAAGAGCGACGAGTGTGTATCTTGTTGACCGT
GTAATTCCGATGATTCCACATCGTCTATCTAACGGTATTTGTTCATTAAATCCGAAAGTAGACCGTCTAACATTATCTTG
TGAAATGGAAATCAACAACTTAGGTGACGTTGTCAAACACGAGATTTTCCAAAGTGTAATTAAAACGACAGAGCGTATGA
CGTATGCTGACGTAAGAAGCATTTTAGAAGATGAGGACGAAGAGCTAATTAAGCGTTATGAACCGTTAGTGCCGATGTTT
AAAGAGATGGGGCAATTAGCACAAATTTTACGTGAAAAACGTATGCGCCGCGGTGCAATCGACTTTGACTTTAAAGAAGC
GAAAGTATTAGTGGATGAAGAAGGAAAACCGACAGATGTTGTCATGCGTGATCGTTCTGTATCAGAGAAGTTAATTGAAG
AATTTATGCTTGTTGCGAACGAAACAGTAGCAGAGCACTTCCACTGGATGAACGTACCATTCATGTATCGTGTCCATGAA
GATCCGAAAGAAGATAAGCTAGAGCGTTTCTTCGAGTTTGTAACGAACTTCGGATATGCCGTAAAAGGACGTGCGAATGA
AGTGCATCCTCGCGCGTTGCAACAAATTCTTGAAATGGTTCAAGGACAGCCTGAAGAGGTAGTTATTTCAACAGTAATGC
TTCGTTCTATGAAGCAAGCACGTTACGATGCAGAAAGTTTAGGTCACTTTGGTTTATCAACTGAGTTCTACACACATTTC
ACATCGCCAATTCGTCGTTACCCAGATACGATTGTTCATAGATTAATTCGTGAATACATCATTAACGGTAAAGTCGACAA
TGAAACGCAAGCAAAATGGCGTGAAAAATTACCTGAGATTGCAGAACACTCTTCTAATATGGAACGTCGTGCTGTTGAAG
CAGAACGTGAAACAGACGAGCTGAAAAAAGCAGAATATATGCTTGATAAGATTGGCGAAGAGTATGACGGTATGATTAGC
TCTGTAACAAACTTCGGTTTATTCGTAGAGCTTCCAAATACAATTGAAGGTCTTGTACATGTTAGCTATTTAACGGATGA
TTACTACCGTTATGATGAGCAGCATTTCGCAATGATTGGAGAACGTACAGGTAACGTATTCCGCATCGGTGACGAAATTA
CAATTCGCGTTATTAACGTAAACAAAGACGAACGTGCAATCGACTTTGAAATCGTTGGTATGAAAGGTACGCCTCGTCGT
AAGTTCAAAGATCGTCCAGTCGTTATCGAACAGCCAAGAACAGGCAGAAAGAAACGCGGTGGACGTAGCGAGCGCAGTAA
TGAGCGCGGCGGAGAACGTGGCACAGGAAGAAAATTTGACCGTGGTGGCAAAGGGAAAGGAAGAGGATCTGCATCCGCAT
CCGCTAGCCAGCCAGGGAAAAAAGATGGTAACGGTAAGAAGAAAAAAGCATTCTTCGAAAACGTACCAGGATTCAAGAAG
AAAAAGAAAAAGCGTAAGTAA

Upstream 100 bases:

>100_bases
ACTGTAAAAATCATAAGGGGAGTATTGAAGTTTCGCTTTATACATCCCCTATCTTTCGGATACACTAAATAATATAAGGT
TTTAAAGGAGGTATTTTTGC

Downstream 100 bases:

>100_bases
GAAAAGGATGGCTTCGCTTTGATAAAAAGCGAGGCTTTTTCTTTTTTATTGGTGGCTTGAGAGCGAGCCACTTTCCTCTT
TTGTTGGAATCCAGCTACAG

Product: ribonuclease R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDAESLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGKKDGNGKKKKAFFENVPGFKK
KKKKRK

Sequences:

>Translated_806_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDAESLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGKKDGNGKKKKAFFENVPGFKK
KKKKRK
>Mature_806_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDAESLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGKKDGNGKKKKAFFENVPGFKK
KKKKRK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014625, Length=462, Percent_Identity=30.952380952381, Blast_Score=227, Evalue=4e-59,
Organism=Homo sapiens, GI190014623, Length=462, Percent_Identity=30.952380952381, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI134288890, Length=414, Percent_Identity=34.0579710144928, Blast_Score=218, Evalue=2e-56,
Organism=Homo sapiens, GI19115966, Length=575, Percent_Identity=28.3478260869565, Blast_Score=193, Evalue=5e-49,
Organism=Homo sapiens, GI219521928, Length=575, Percent_Identity=28.3478260869565, Blast_Score=193, Evalue=7e-49,
Organism=Escherichia coli, GI87082383, Length=725, Percent_Identity=39.0344827586207, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1787542, Length=639, Percent_Identity=25.0391236306729, Blast_Score=149, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17553506, Length=473, Percent_Identity=31.0782241014799, Blast_Score=205, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI212645896, Length=492, Percent_Identity=28.8617886178862, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324552, Length=589, Percent_Identity=28.0135823429542, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320499, Length=422, Percent_Identity=25.3554502369668, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323943, Length=432, Percent_Identity=23.1481481481481, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24649634, Length=475, Percent_Identity=31.3684210526316, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI19922976, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24654597, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24654592, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 91885; Mature: 91885

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLVRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDAESLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGK
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KDGNGKKKKAFFENVPGFKKKKKKRK
CCCCCCHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLVRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDAESLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDELKKAEYMLDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASASQPGK
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KDGNGKKKKAFFENVPGFKKKKKKRK
CCCCCCHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]