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The map label for this gene is bfmbB [H]

Identifier: 118479353

GI number: 118479353

Start: 3981722

End: 3983068

Strand: Reverse

Name: bfmbB [H]

Synonym: BALH_3770

Alternate gene names: 118479353

Gene position: 3983068-3981722 (Counterclockwise)

Preceding gene: 118479354

Following gene: 118479352

Centisome position: 75.77

GC content: 40.76

Gene sequence:

>1347_bases
TTGGGGGGACTATACATGGCTGTAGAAAATATTACAATGCCTCAGCTCGGGGAGAGCGTTACAGAGGGTACAATTAGTAA
ATGGCTCGTTAATGTTGGCGATCACGTAAACAAATATGATCCGCTTGCAGAAGTAATGACTGATAAAGTAAATGCTGAAG
TACCATCTTCTTTCACTGGTATTGTGAAAGAGTTGATCGCTGGTGAAGGTGATACGTTAGCTGTAGGTGAAGTAGTTTGT
GTGATTCAAGTAGAAGGCGCAGATGAAGTAGCAGCAACAGCTGTTGAGGAAAAAACAAAAGAAGAACCAAAAGAAGAACC
AAAAGCAGAAGTAGCTACGCCTGAAAAAGCACCGAAAGCAAAACAACCAACTGATGGAAAACCTCGTTTCTCACCAGCTG
TGTTAAAACTTGCAGGTGAACATAACGTTGATTTAGATCTAGTAGAAGGTACGGGAGCAAACGGCCGTATCACTCGTAAA
GATATTTTAAAGCTAGTGGAATCAGGAAATATTCCGCAAGCAGGTGCGGCGAAGAAAGAGGAAGCGGTAGCGGCAGTAGT
AGAAGCGCGTCCAGAAGCACCAAAAGCAGCGCCAATAGCACAAAAAGTAGAAGCTGCGAAACCAGTTTCTGTACCAACAA
TGCCTGGCGATATCGAAATTCCAGTAACAGGTGTGCGTAAAGCAATCGCAGCGAACATGTTACGTAGTAAACACGAAGCG
CCACACGCTTGGATGATGATTGAAGTAGATGTGACAAACCTTGTGTCATACCGTAATTCAATTAAAGGTGATTTCAAAAA
ACGCGAAGGCTTTAACTTAACGTTCTTTGCTTTCTTCGTAAAAGCAGTAGCACAAGCGTTAAAAGAGTACCCTCAAATTA
ATTCAATGTGGGCTGGCGATAAAATCGTTCAGAAGAAAGATATTAACCTTTCTATCGCCGTTGCGACAGAGGACGAACTA
TTTGTACCAGTAATTAAACACGCGGACGAGAAGACAATTAAAGGTATCGCTCGTGAAATTACAGAACTTGCAGGAAAAGT
ACGTACGAAATCGTTAAAAGCGGACGAAATGCAAGGCGGAACATTTACAATTAATAACACAGGATCATTCGGTTCTGTTC
AATCTATGGGTATTATTAATTACCCACAAGCGGCTATTTTACAAGTTGAATCAATTGTAAAACGCCCAGTAATTATGGAA
AATGGTATGTTCGGTGCTCGCGACATGGTTAACTTATGTTTATCACTCGATCACCGTGTACTAGATGGTTTAATTTGTGG
TAAGTTCTTAGGACGTGTAAAAGAAATTTTAGAAAATACGTCAGAAAATAATACGTCTGTATATTAA

Upstream 100 bases:

>100_bases
AGACGTTCCAGCAATGCCATATGCACCAACAATGGAAAAATTCTTTATGGTAAATCCAGATAAAGTTGAAAAAGCAATGC
GTGAACTTGCGGAATTTTAA

Downstream 100 bases:

>100_bases
ATAAAAAGCTCGCTTATGCGAGCTTTTTATCATCTTATTTCTCCATCTACATCCTACTATATGTATAATACCTTTCCCAG
TAATCTTACAATGAACGATT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MGGLYMAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKELIAGEGDTLAVGEVVC
VIQVEGADEVAATAVEEKTKEEPKEEPKAEVATPEKAPKAKQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRK
DILKLVESGNIPQAGAAKKEEAVAAVVEARPEAPKAAPIAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEA
PHAWMMIEVDVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEDEL
FVPVIKHADEKTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIME
NGMFGARDMVNLCLSLDHRVLDGLICGKFLGRVKEILENTSENNTSVY

Sequences:

>Translated_448_residues
MGGLYMAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKELIAGEGDTLAVGEVVC
VIQVEGADEVAATAVEEKTKEEPKEEPKAEVATPEKAPKAKQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRK
DILKLVESGNIPQAGAAKKEEAVAAVVEARPEAPKAAPIAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEA
PHAWMMIEVDVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEDEL
FVPVIKHADEKTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIME
NGMFGARDMVNLCLSLDHRVLDGLICGKFLGRVKEILENTSENNTSVY
>Mature_447_residues
GGLYMAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTGIVKELIAGEGDTLAVGEVVCV
IQVEGADEVAATAVEEKTKEEPKEEPKAEVATPEKAPKAKQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRKD
ILKLVESGNIPQAGAAKKEEAVAAVVEARPEAPKAAPIAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEAP
HAWMMIEVDVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGDKIVQKKDINLSIAVATEDELF
VPVIKHADEKTIKGIAREITELAGKVRTKSLKADEMQGGTFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIMEN
GMFGARDMVNLCLSLDHRVLDGLICGKFLGRVKEILENTSENNTSVY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=456, Percent_Identity=30.9210526315789, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=30.58568329718, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=30.58568329718, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI110671329, Length=439, Percent_Identity=29.3849658314351, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI19923748, Length=217, Percent_Identity=36.8663594470046, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=36.9426751592357, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=33.1797235023041, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1786305, Length=441, Percent_Identity=33.1065759637188, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=440, Percent_Identity=28.8636363636364, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=434, Percent_Identity=29.2626728110599, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=455, Percent_Identity=27.4725274725275, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=28.6624203821656, Blast_Score=121, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=430, Percent_Identity=30.9302325581395, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=27.0152505446623, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=30.184331797235, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI20129315, Length=225, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24582497, Length=225, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=34.8837209302326, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 48359; Mature: 48228

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLYMAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTG
CCCEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCCHHHH
IVKELIAGEGDTLAVGEVVCVIQVEGADEVAATAVEEKTKEEPKEEPKAEVATPEKAPKA
HHHHHHCCCCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCC
KQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRKDILKLVESGNIPQAGAAKKE
CCCCCCCCCCCHHHHEECCCCCCCEEEEECCCCCCEECHHHHHHHHHCCCCCCCCCCHHH
EAVAAVVEARPEAPKAAPIAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEA
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCC
PHAWMMIEVDVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGD
CCEEEEEEEEHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
KIVQKKDINLSIAVATEDELFVPVIKHADEKTIKGIAREITELAGKVRTKSLKADEMQGG
HHEEEECCCEEEEEECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
TFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIMENGMFGARDMVNLCLSLDHRV
EEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHH
LDGLICGKFLGRVKEILENTSENNTSVY
HHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GGLYMAVENITMPQLGESVTEGTISKWLVNVGDHVNKYDPLAEVMTDKVNAEVPSSFTG
CCEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCCHHHH
IVKELIAGEGDTLAVGEVVCVIQVEGADEVAATAVEEKTKEEPKEEPKAEVATPEKAPKA
HHHHHHCCCCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCC
KQPTDGKPRFSPAVLKLAGEHNVDLDLVEGTGANGRITRKDILKLVESGNIPQAGAAKKE
CCCCCCCCCCCHHHHEECCCCCCCEEEEECCCCCCEECHHHHHHHHHCCCCCCCCCCHHH
EAVAAVVEARPEAPKAAPIAQKVEAAKPVSVPTMPGDIEIPVTGVRKAIAANMLRSKHEA
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCC
PHAWMMIEVDVTNLVSYRNSIKGDFKKREGFNLTFFAFFVKAVAQALKEYPQINSMWAGD
CCEEEEEEEEHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
KIVQKKDINLSIAVATEDELFVPVIKHADEKTIKGIAREITELAGKVRTKSLKADEMQGG
HHEEEECCCEEEEEECCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
TFTINNTGSFGSVQSMGIINYPQAAILQVESIVKRPVIMENGMFGARDMVNLCLSLDHRV
EEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHH
LDGLICGKFLGRVKEILENTSENNTSVY
HHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]