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The map label for this gene is bioB

Identifier: 118479315

GI number: 118479315

Start: 3944662

End: 3945660

Strand: Reverse

Name: bioB

Synonym: BALH_3730

Alternate gene names: 118479315

Gene position: 3945660-3944662 (Counterclockwise)

Preceding gene: 118479316

Following gene: 118479314

Centisome position: 75.05

GC content: 38.14

Gene sequence:

>999_bases
ATGAAACAAGTACAAACAAAAAGGGATTGGAAAAAACTTGCATATGACGTAGTAGAAGAAAAAATGATTGCAAAAGAGGA
TGCAATAGCGATATTAGAAGCTGAGGATACAGAAGTTTTAGAGATTATGAATGCTGCTTACATTATTCGCCATCATTACT
TTGGTAAGAAAGTAAAATTAAATATGATTATTAATACGAAATCTGGATTATGTCCTGAAGATTGCGGATATTGTTCACAG
TCGATTATTTCAGAAGCACCAATCGATAAATATGCATGGCTAACGCAGGAAAAGATTGTAGAAGGAGCACACGAAGCAAT
ACGCCGTAAAGCTGGTACATATTGTATTGTAGCATCTGGTCGTCGCCCGACGGATAAAGAGGTGAATCACGTAATTGGAG
CAGTTAAGGAGATTCGTGAAACGACAGATTTGAAGATTTGTTGTTGTTTAGGTTTCTTAAACGAAGATCAAGCAGGACGT
TTAGCTGAAGCTGGTGTACATCGTTATAACCACAACTTAAATACACATGCGAATAATTACGAAAACATTTGCTCCACTCA
TACGTATGATGATCGTGTTGATACAGTTCAAAAAGCGAAACAAGCGGGTATTTCACCATGTTCTGGGGCAATCTTTGGAA
TGGGAGAGACAATAGAAGAGCGCGCTGAAATTGCATTCGAATTACAACGTATAGATGCAGATTCAATTCCATGTAATTTC
CTTGTTGCTGTAAAGGGTACGCCGCTTGAAGGACAAAAGGAGTTAACGCCAGTAGAATGTTTAAAAGTGTTGGCAATGAT
GCGTTTTGTAAACCCAACAAAAGAAATTCGTATTTCAGGAGGGCGTGAAATCAATTTACGTTCTGTACAGCCAATCGGTT
TATTTGCAGCAAACTCTATTTTTGTTGGAGACTACTTAACGACAGCTGGACAAGAACCGACTGCAGACTGGGGTATGATT
GAAGATTTAGGATTTGAGATTGAAGAATGTGCGTTATAA

Upstream 100 bases:

>100_bases
ATGCTTCGCATATACGAAAGAGACTTCACGGAAAATGAAGGGATAATGGCGACGTATCACGCTTTATTTATGCATATAAC
AAAAGAGGGGAAGAGATGAA

Downstream 100 bases:

>100_bases
GATAGAGGAAAAAGTAAACTTGTAAAAGAGTTTGCTTTTTTTGTAAATAAAATGTAATAAATAGTTGTGAACGCTTGATA
CTAGTGGCTTTTGAGCGAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKQVQTKRDWKKLAYDVVEEKMIAKEDAIAILEAEDTEVLEIMNAAYIIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGR
LAEAGVHRYNHNLNTHANNYENICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSIFVGDYLTTAGQEPTADWGMI
EDLGFEIEECAL

Sequences:

>Translated_332_residues
MKQVQTKRDWKKLAYDVVEEKMIAKEDAIAILEAEDTEVLEIMNAAYIIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGR
LAEAGVHRYNHNLNTHANNYENICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSIFVGDYLTTAGQEPTADWGMI
EDLGFEIEECAL
>Mature_332_residues
MKQVQTKRDWKKLAYDVVEEKMIAKEDAIAILEAEDTEVLEIMNAAYIIRHHYFGKKVKLNMIINTKSGLCPEDCGYCSQ
SIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASGRRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGR
LAEAGVHRYNHNLNTHANNYENICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSIFVGDYLTTAGQEPTADWGMI
EDLGFEIEECAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=295, Percent_Identity=34.5762711864407, Blast_Score=191, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321725, Length=309, Percent_Identity=32.0388349514563, Blast_Score=159, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_BACAH (A0RIB6)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_896466.1
- ProteinModelPortal:   A0RIB6
- SMR:   A0RIB6
- STRING:   A0RIB6
- EnsemblBacteria:   EBBACT00000066993
- GeneID:   4543237
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_3730
- NMPDR:   fig|412694.5.peg.3589
- eggNOG:   COG0502
- GeneTree:   EBGT00050000002917
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   PRK06256
- BioCyc:   BTHU412694:BALH_3730-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37008; Mature: 37008

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVQTKRDWKKLAYDVVEEKMIAKEDAIAILEAEDTEVLEIMNAAYIIRHHYFGKKVKL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEE
NMIINTKSGLCPEDCGYCSQSIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASG
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
RRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGRLAEAGVHRYNHNLNTHANNY
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCH
ENICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSI
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCEEEEECCE
FVGDYLTTAGQEPTADWGMIEDLGFEIEECAL
EECCHHHHCCCCCCCCCCCHHHHCCCHHHHCC
>Mature Secondary Structure
MKQVQTKRDWKKLAYDVVEEKMIAKEDAIAILEAEDTEVLEIMNAAYIIRHHYFGKKVKL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEE
NMIINTKSGLCPEDCGYCSQSIISEAPIDKYAWLTQEKIVEGAHEAIRRKAGTYCIVASG
EEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
RRPTDKEVNHVIGAVKEIRETTDLKICCCLGFLNEDQAGRLAEAGVHRYNHNLNTHANNY
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCH
ENICSTHTYDDRVDTVQKAKQAGISPCSGAIFGMGETIEERAEIAFELQRIDADSIPCNF
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
LVAVKGTPLEGQKELTPVECLKVLAMMRFVNPTKEIRISGGREINLRSVQPIGLFAANSI
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCEEEEECCE
FVGDYLTTAGQEPTADWGMIEDLGFEIEECAL
EECCHHHHCCCCCCCCCCCHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA