BacMap

Definition	Bacillus thuringiensis str. Al Hakam chromosome, complete genome.
Accession	NC_008600
Length	5,257,091

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The map label for this gene is ytbE [H]

Identifier: 118479299

GI number: 118479299

Start: 3930205

End: 3931098

Strand: Reverse

Name: ytbE [H]

Synonym: BALH_3714

Alternate gene names: 118479299

Gene position: 3931098-3930205 (Counterclockwise)

Preceding gene: 118479300

Following gene: 118479297

Centisome position: 74.78

GC content: 36.8

Gene sequence:

>894_bases
TTGGTTGAATCTTGTTCAAATAAAGTGGTGGTTTATAAAAAATGTAATGATGAAAGGGAGACGACTGTGCACATTCCAAC
AACTACACTTCATAATGGCGTAAAAATGCCGATGATTGGTTTAGGCGTTTATAAAGCGAAAGAAGGCGACGAAGTAAAAC
AAGCAGTAAAAACAGCGCTAGAAGTTGGATACCGTTCGATTGATACAGCAACTGTATATGAAAATGAAAGCGGTGTCGGA
GAAGCGGTTCGTGAATCAGGAATTCCGAGAGAAGACATCTTTATTACAACAAAAGTTTGGAACGACGATCAAGGATACGA
GGAAACACTTGAGGCGTTTGAGAAAAGCTTAAAAAAATTACAAATGGACTATGTAGATTTATATTTAATACATTGGCCAA
TAAGAGGGAAGTATGTTGATACGTACCGAGCATTAGAAAAACTGTATGAAGAAGGTAAAGTGCGCGCGATTGGTGTTTCG
AATTTCCATAAACATCATTTAGAACTGCTATTACCAAATTGCAAAATAAAGCCGATGGTAAACCAAGTGGAACTTCATCC
CATGTTAACACAATTTGAATTGCGTAATTTCTGTCAAGGTGAGCAAATTCAAATGGAAGCATGGAGTCCATTAATGAGAG
GTGGAGAAGTATTTCAGCACCCGATTATTCAGGCCATTGCTACTAAATATGAAAAAACACCTGCGCAAGTTATATTAAGG
TGGGATATTCAAAGCGGGATTGTGACGATTCCTAAGTCTGTTACACCATCTCGCATTCAGGAGAATTTTTCTATCTTTGA
CTTTTCACTAACAGAAGAAGAGATGACTCAAATTAATACGTTAAATCGTAATTTACATGTAGGAACGAATCCTGATAAAT
ATGATACGTTATAA

Upstream 100 bases:

>100_bases
AAGCGAAGAAATGGATAGAAAATAGCGCGAATGAATGAGTTCGCATTATTTTTGAATATGTATAAATTTATTATGTAAAC
TGTACATATGTTTTGACTTT

Downstream 100 bases:

>100_bases
AAAGACGCTACCAGTATGGTAGCGTCTTTTTAACGATGTAAAGCGTACGTATCACATTTCGCAATTTGCTGAAGCTGTAT
ATATTCTTCTGTTGTTAACT

Product: aldo/keto reductase family oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MVESCSNKVVVYKKCNDERETTVHIPTTTLHNGVKMPMIGLGVYKAKEGDEVKQAVKTALEVGYRSIDTATVYENESGVG
EAVRESGIPREDIFITTKVWNDDQGYEETLEAFEKSLKKLQMDYVDLYLIHWPIRGKYVDTYRALEKLYEEGKVRAIGVS
NFHKHHLELLLPNCKIKPMVNQVELHPMLTQFELRNFCQGEQIQMEAWSPLMRGGEVFQHPIIQAIATKYEKTPAQVILR
WDIQSGIVTIPKSVTPSRIQENFSIFDFSLTEEEMTQINTLNRNLHVGTNPDKYDTL

Sequences:

>Translated_297_residues
MVESCSNKVVVYKKCNDERETTVHIPTTTLHNGVKMPMIGLGVYKAKEGDEVKQAVKTALEVGYRSIDTATVYENESGVG
EAVRESGIPREDIFITTKVWNDDQGYEETLEAFEKSLKKLQMDYVDLYLIHWPIRGKYVDTYRALEKLYEEGKVRAIGVS
NFHKHHLELLLPNCKIKPMVNQVELHPMLTQFELRNFCQGEQIQMEAWSPLMRGGEVFQHPIIQAIATKYEKTPAQVILR
WDIQSGIVTIPKSVTPSRIQENFSIFDFSLTEEEMTQINTLNRNLHVGTNPDKYDTL
>Mature_297_residues
MVESCSNKVVVYKKCNDERETTVHIPTTTLHNGVKMPMIGLGVYKAKEGDEVKQAVKTALEVGYRSIDTATVYENESGVG
EAVRESGIPREDIFITTKVWNDDQGYEETLEAFEKSLKKLQMDYVDLYLIHWPIRGKYVDTYRALEKLYEEGKVRAIGVS
NFHKHHLELLLPNCKIKPMVNQVELHPMLTQFELRNFCQGEQIQMEAWSPLMRGGEVFQHPIIQAIATKYEKTPAQVILR
WDIQSGIVTIPKSVTPSRIQENFSIFDFSLTEEEMTQINTLNRNLHVGTNPDKYDTL

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=41.8685121107266, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=41.8685121107266, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI223468663, Length=292, Percent_Identity=43.8356164383562, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI93277124, Length=304, Percent_Identity=38.1578947368421, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI310109922, Length=278, Percent_Identity=40.6474820143885, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI5174695, Length=295, Percent_Identity=40.3389830508475, Blast_Score=207, Evalue=6e-54,
Organism=Homo sapiens, GI5453543, Length=298, Percent_Identity=38.5906040268456, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=38.9261744966443, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=38.9261744966443, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=38.5906040268456, Blast_Score=204, Evalue=6e-53,
Organism=Homo sapiens, GI4502049, Length=293, Percent_Identity=43.0034129692833, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI291291012, Length=281, Percent_Identity=41.6370106761566, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI24497583, Length=295, Percent_Identity=37.9661016949153, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI24497585, Length=293, Percent_Identity=37.542662116041, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=37.8181818181818, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI300116271, Length=273, Percent_Identity=38.8278388278388, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI310109924, Length=157, Percent_Identity=38.8535031847134, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=46.4912280701754, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=45.6140350877193, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87082198, Length=268, Percent_Identity=48.134328358209, Blast_Score=276, Evalue=8e-76,
Organism=Escherichia coli, GI1786400, Length=255, Percent_Identity=35.6862745098039, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1788081, Length=262, Percent_Identity=31.6793893129771, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788070, Length=317, Percent_Identity=25.5520504731861, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081735, Length=175, Percent_Identity=31.4285714285714, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17537077, Length=297, Percent_Identity=40.0673400673401, Blast_Score=210, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI71998625, Length=268, Percent_Identity=41.4179104477612, Blast_Score=206, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI17564128, Length=295, Percent_Identity=38.6440677966102, Blast_Score=204, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17537075, Length=305, Percent_Identity=39.344262295082, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=36.4963503649635, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17550248, Length=282, Percent_Identity=38.6524822695035, Blast_Score=183, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17566692, Length=293, Percent_Identity=37.542662116041, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=39.3382352941176, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17562292, Length=276, Percent_Identity=36.231884057971, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=37.956204379562, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17552492, Length=263, Percent_Identity=31.9391634980989, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537079, Length=304, Percent_Identity=31.25, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324694, Length=286, Percent_Identity=40.5594405594406, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319625, Length=302, Percent_Identity=40.3973509933775, Blast_Score=199, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=38.1944444444444, Blast_Score=198, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=35.9477124183007, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6322556, Length=257, Percent_Identity=35.408560311284, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320079, Length=286, Percent_Identity=34.2657342657343, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24663317, Length=286, Percent_Identity=41.958041958042, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24657054, Length=282, Percent_Identity=43.2624113475177, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=38.3333333333333, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=40, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=42.0689655172414, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=35.9375, Blast_Score=203, Evalue=9e-53,
Organism=Drosophila melanogaster, GI45553081, Length=288, Percent_Identity=39.9305555555556, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI20129731, Length=290, Percent_Identity=39.3103448275862, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24662781, Length=280, Percent_Identity=41.0714285714286, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24662785, Length=279, Percent_Identity=40.8602150537634, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI281366140, Length=252, Percent_Identity=38.8888888888889, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 34098; Mature: 34098

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVESCSNKVVVYKKCNDERETTVHIPTTTLHNGVKMPMIGLGVYKAKEGDEVKQAVKTAL
CCCCCCCCEEEEEECCCCCCEEEEECCHHHCCCCCCCEEECEEEECCCCHHHHHHHHHHH
EVGYRSIDTATVYENESGVGEAVRESGIPREDIFITTKVWNDDQGYEETLEAFEKSLKKL
HHCCCCCCEEEEEECCCCHHHHHHHCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHH
QMDYVDLYLIHWPIRGKYVDTYRALEKLYEEGKVRAIGVSNFHKHHLELLLPNCKIKPMV
HHHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHEEECCCCCCCCCC
NQVELHPMLTQFELRNFCQGEQIQMEAWSPLMRGGEVFQHPIIQAIATKYEKTPAQVILR
CCEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEE
WDIQSGIVTIPKSVTPSRIQENFSIFDFSLTEEEMTQINTLNRNLHVGTNPDKYDTL
EECCCCEEECCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHCCEEEECCCCCCCCCC
>Mature Secondary Structure
MVESCSNKVVVYKKCNDERETTVHIPTTTLHNGVKMPMIGLGVYKAKEGDEVKQAVKTAL
CCCCCCCCEEEEEECCCCCCEEEEECCHHHCCCCCCCEEECEEEECCCCHHHHHHHHHHH
EVGYRSIDTATVYENESGVGEAVRESGIPREDIFITTKVWNDDQGYEETLEAFEKSLKKL
HHCCCCCCEEEEEECCCCHHHHHHHCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHH
QMDYVDLYLIHWPIRGKYVDTYRALEKLYEEGKVRAIGVSNFHKHHLELLLPNCKIKPMV
HHHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHEEECCCCCCCCCC
NQVELHPMLTQFELRNFCQGEQIQMEAWSPLMRGGEVFQHPIIQAIATKYEKTPAQVILR
CCEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEE
WDIQSGIVTIPKSVTPSRIQENFSIFDFSLTEEEMTQINTLNRNLHVGTNPDKYDTL
EECCCCEEECCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]