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The map label for this gene is nplT [H]

Identifier: 118479222

GI number: 118479222

Start: 3858718

End: 3860478

Strand: Direct

Name: nplT [H]

Synonym: BALH_3634

Alternate gene names: 118479222

Gene position: 3858718-3860478 (Clockwise)

Preceding gene: 118479217

Following gene: 118479224

Centisome position: 73.4

GC content: 35.83

Gene sequence:

>1761_bases
ATGCTTAAAGAAGCCATTTATCATAGGCCAAAAGATAATTACGCATACGCTTACGATGAAAAAACGATTCACATCCGAAT
ACGTACGAAAAGAGACGATGTTCAAAGCACCACTCTTATTTATGGTGATCCTTATGAATGGAAAGACGGGAAGTGGATTT
CTTCAAGTACACCGATGAAAAAAACTGGTTCTACTGCATTGTTTGATTATTGGTTCATTTCCATCGAGCCGAAATTTAAG
CGCTTACGTTATGGATTTGAATTAAAAAACGATACAGACACTCTTATTTATGCAGAGCGAGGATTTTTTTCTACCACTCC
AAATGATGACGTTGGTAACTTTTTCTGTTTTCCATTTATTCATGCAAATGATGTATTCAAAGCACCTTCTTGGATTAAAG
ATACTGTTTGGTATCAAATTTTCCCAGAACGTTTCGCTAATGGAGATTGTACACTGAATCCAAAAAACACCCTTCCTTGG
GGCAGCACTGAGCCAACTCCAACTAATTTTTTCGGTGGAGATTTTGCTGGTATTATTCAAAACCTTGATTACCTTGTTAA
GCTCGGAATTTCTGGAATATATTTCACACCTATTTTCAAAGCACATTCAAACCATAAATATGACACAATTGACTACATGG
AAATCGATCCGCAATTTGGCACAAAAAAAACATTCAAAGAACTCGTTCAGGCATGTCATACACACGGTATAAAAGTAATG
CTCGATGCCGTATTCAATCACAGTGGATACTTTTTCGATAAATTTCAAGACGTACTACAAAATGGTGAGCAGTCAGCATA
TAAAGAATGGTTCCATATTCATGAGTTCCCAATTAGAACTGAGCCACTCCCGAATTATGATACTTTCGCGTTTACACCGT
ATATGCCAAAGTTAAACACAGCGCACCCGGATGTAAAAGAATACTTACTTGAAGTAGGGCGTTATTGGGTAAGAGAATTC
AATATAGACGGCTGGCGCCTTGATGTAGCAAATGAAGTCGACCATAACTTTTGGAGAGAATTTCGAAGTGAGATAAAAGC
ATTAAATTCTGAAGTATATATTTTAGGAGAAATTTGGCACGATGCACTTCCATGGCTACAAGGAGATCAATTCGATGCTG
TCATGAGCTATCCTGTTACAAACGCCCTACTGTCTTACTTTGCTAACGATTCCATTAAGGCAAATGAATTTATGAAACAA
ATTACAGAATCTCTACATTCCTACTCTATGAATGTAAATGAAGCAGCATTTCATTTATTAGATAGCCATGATACACCGAG
AATTTTGACAACATGTAACGGAGATAAAAATAAGTTACAATTACTTTATGTATTCCATCTTTCTTTCATCGGCTCTCCTT
GTATTTATTATGGAGACGAAATTGGTATGGACGGCGGTATGGACCCAGATTGTCGCAAATGTATGGTTTGGGATACGGAA
GAACAAGATCATACATTATTTACACATGTACAAACATTAATTTCATTACGAAAGCAATATAAAGCATTTGGAGGACATGG
TACTTTCCAATTCATTGAAGCAAATGATGAATATAATTATATTTCTTATACGAAAACATATGAAGATGAAACGATCTTTT
TCGTTTTAAATCCGACTAATAGTGATATTACAGCTTCACTCCCTCTCCATGTTACTGGAAAGAAAATCATTAACATTTAC
ACAAACGAAGAATTTTCAGCGGAAGCAAGTGTATTACAAGTTACACTTCCTCCATATGGATTCTCCATATTAAAATGGTA
A

Upstream 100 bases:

>100_bases
AATATTAAAGCTATGCAAAAAGCAATCGTTTGCAACATATGCTTTATTATTATACGTAAGGGAACAATATGCATGAAGAT
AGTAAAGGGGGATTTTCTAT

Downstream 100 bases:

>100_bases
TCAAAAAATCCTTATACCAAAACGGTATAAGGATTTTTCTTATTTCAATTTAAATACCATTGCTTCATATGGACGTAATG
TTATGTTTTCTATCGGTCCA

Product: neopullulanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMKKTGSTALFDYWFISIEPKFK
RLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFIHANDVFKAPSWIKDTVWYQIFPERFANGDCTLNPKNTLPW
GSTEPTPTNFFGGDFAGIIQNLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKKTFKELVQACHTHGIKVM
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNTAHPDVKEYLLEVGRYWVREF
NIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWHDALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQ
ITESLHSYSMNVNEAAFHLLDSHDTPRILTTCNGDKNKLQLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTNSDITASLPLHVTGKKIINIY
TNEEFSAEASVLQVTLPPYGFSILKW

Sequences:

>Translated_586_residues
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMKKTGSTALFDYWFISIEPKFK
RLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFIHANDVFKAPSWIKDTVWYQIFPERFANGDCTLNPKNTLPW
GSTEPTPTNFFGGDFAGIIQNLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKKTFKELVQACHTHGIKVM
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNTAHPDVKEYLLEVGRYWVREF
NIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWHDALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQ
ITESLHSYSMNVNEAAFHLLDSHDTPRILTTCNGDKNKLQLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTNSDITASLPLHVTGKKIINIY
TNEEFSAEASVLQVTLPPYGFSILKW
>Mature_586_residues
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMKKTGSTALFDYWFISIEPKFK
RLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFIHANDVFKAPSWIKDTVWYQIFPERFANGDCTLNPKNTLPW
GSTEPTPTNFFGGDFAGIIQNLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKKTFKELVQACHTHGIKVM
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNTAHPDVKEYLLEVGRYWVREF
NIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWHDALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQ
ITESLHSYSMNVNEAAFHLLDSHDTPRILTTCNGDKNKLQLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTNSDITASLPLHVTGKKIINIY
TNEEFSAEASVLQVTLPPYGFSILKW

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=409, Percent_Identity=23.2273838630807, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1786604, Length=507, Percent_Identity=31.163708086785, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI1790687, Length=394, Percent_Identity=25.6345177664975, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789995, Length=484, Percent_Identity=23.1404958677686, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32565753, Length=119, Percent_Identity=31.0924369747899, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=233, Percent_Identity=25.3218884120172, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=405, Percent_Identity=20.9876543209877, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=409, Percent_Identity=21.0268948655257, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321726, Length=229, Percent_Identity=24.0174672489083, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322241, Length=229, Percent_Identity=24.0174672489083, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=229, Percent_Identity=24.0174672489083, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324416, Length=229, Percent_Identity=24.0174672489083, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586587, Length=426, Percent_Identity=23.4741784037559, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24586591, Length=408, Percent_Identity=22.7941176470588, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24586593, Length=410, Percent_Identity=22.4390243902439, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586589, Length=403, Percent_Identity=24.0694789081886, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24583747, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583749, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586599, Length=223, Percent_Identity=25.5605381165919, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330053, Length=181, Percent_Identity=27.6243093922652, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45549022, Length=246, Percent_Identity=24.7967479674797, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583745, Length=223, Percent_Identity=26.9058295964126, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=25.3333333333333, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 68258; Mature: 68258

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMK
CCCHHHHCCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEECCCCCCCCCEEECCCCCCC
KTGSTALFDYWFISIEPKFKRLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFI
CCCCEEEEEEEEEEECCHHHHHEECEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEE
HANDVFKAPSWIKDTVWYQIFPERFANGDCTLNPKNTLPWGSTEPTPTNFFGGDFAGIIQ
ECCCCCCCCCHHHHHHHEEECHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
NLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKKTFKELVQACHTHGIKVM
HHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEE
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNT
EEHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCC
AHPDVKEYLLEVGRYWVREFNIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWH
CCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
DALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQITESLHSYSMNVNEAAFHLL
HHCCCCCCCCCCHHECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
DSHDTPRILTTCNGDKNKLQLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
CCCCCCEEEEECCCCCCCEEEEEEEEHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCC
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTN
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCCC
SDITASLPLHVTGKKIINIYTNEEFSAEASVLQVTLPPYGFSILKW
CCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEEECCCCCEECCC
>Mature Secondary Structure
MLKEAIYHRPKDNYAYAYDEKTIHIRIRTKRDDVQSTTLIYGDPYEWKDGKWISSSTPMK
CCCHHHHCCCCCCEEEEECCEEEEEEEEECCCCCCCEEEEECCCCCCCCCEEECCCCCCC
KTGSTALFDYWFISIEPKFKRLRYGFELKNDTDTLIYAERGFFSTTPNDDVGNFFCFPFI
CCCCEEEEEEEEEEECCHHHHHEECEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEE
HANDVFKAPSWIKDTVWYQIFPERFANGDCTLNPKNTLPWGSTEPTPTNFFGGDFAGIIQ
ECCCCCCCCCHHHHHHHEEECHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
NLDYLVKLGISGIYFTPIFKAHSNHKYDTIDYMEIDPQFGTKKTFKELVQACHTHGIKVM
HHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEE
LDAVFNHSGYFFDKFQDVLQNGEQSAYKEWFHIHEFPIRTEPLPNYDTFAFTPYMPKLNT
EEHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCC
AHPDVKEYLLEVGRYWVREFNIDGWRLDVANEVDHNFWREFRSEIKALNSEVYILGEIWH
CCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
DALPWLQGDQFDAVMSYPVTNALLSYFANDSIKANEFMKQITESLHSYSMNVNEAAFHLL
HHCCCCCCCCCCHHECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
DSHDTPRILTTCNGDKNKLQLLYVFHLSFIGSPCIYYGDEIGMDGGMDPDCRKCMVWDTE
CCCCCCEEEEECCCCCCCEEEEEEEEHHHHCCCCEEECCCCCCCCCCCCCCCEEEEECCC
EQDHTLFTHVQTLISLRKQYKAFGGHGTFQFIEANDEYNYISYTKTYEDETIFFVLNPTN
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCCC
SDITASLPLHVTGKKIINIYTNEEFSAEASVLQVTLPPYGFSILKW
CCEEEEEEEEECCCEEEEEEECCCCCCCCEEEEEEECCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]