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The map label for this gene is ykuM [H]

Identifier: 118479193

GI number: 118479193

Start: 3827224

End: 3828108

Strand: Reverse

Name: ykuM [H]

Synonym: BALH_3604

Alternate gene names: 118479193

Gene position: 3828108-3827224 (Counterclockwise)

Preceding gene: 118479194

Following gene: 118479192

Centisome position: 72.82

GC content: 33.9

Gene sequence:

>885_bases
ATGGAAGCTAAGGAAAGAGGGAGAAAGTTGCAAATTGATGATTTTCAAATGATGGTTGTGTTAGCACAGGAATCAAATAT
GAGAAAAGCGGCGGAACGTTTATTTGTTTCACAACCTGCGCTTAGTCAACGATTACAATCTATGGAGAAACAATGGGGAA
TGAAGTTTTTTATTCGCTCGCAAAAAGGATTGACAATTACACCTGAAGGGGAAAAAGTTGCAAATTACGCAAAAGAAATG
CTACAAAAGGAAGAGGAAATTAAAAGTGAGTTAGCTGTTTTTAGAACAGAAACGCACGGGACGTTAAAAATAGCTGTCGC
ATCAGTTATTGGTCAATATTGGCTTCCTCCTGTTTTGAAGAAGTTCGTGCATAAATATCCTTCTGTGAAAATATCACTGT
TTACTGGCTGGAGTAGTGAAGTACAAAAGCAGTTTTATGAAGGAGACGTTCATGTTGCTATATTGAGAGGAACACAAGAA
TATAAAGGCCAGAAGCAACAATTATTTGAAGATGAACTTTATTTAGTGGATAAAGAAATAAAAGATATTTCGATGTTAAA
AGAAACAAATAGACCATTTATTCAATTTAAAAGTGACTCGACTTATTATGGACAAATACAAAATTGGTGGTATGGTTTAT
TTTCTAATCCACCTAAGAGAACAATTGTCGTTGATCAAATTGAGACTTGTAAACAACTCGTTTTAAATGGCATAGGCTAC
GCGCTTTTACCTTCTACTGTTTTAAAAGAAGTACAAGAAAATATGTATAAAACACCTGTTCAATTAACGAGAGAAACATG
GTTATTGACGAGTGAATCAGCTAGACAATTGAAGCAAGTACAAGCATTTTTAGAAATTATAGAAGAAATTCAAATGGAAA
AATAG

Upstream 100 bases:

>100_bases
TTTCGTGCATATCAGTTGTGTATGTATTATATTTAAAAAGATAAAAAATATAAGAGTATGTAGTTAAATTAAGGATGAAA
AGCTTATTGAGATAAGGTGT

Downstream 100 bases:

>100_bases
GATTATTATCTTGCTACTCGCTCTATCGTTTGGTAGAGTAACATTATAAATGGATTTAGGAGGCAACAAGAATGAAAATG
ATGGACGCTAACGAAATTAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQKEEEIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK

Sequences:

>Translated_294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQKEEEIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK
>Mature_294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQKEEEIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK

Specific function: This Protein Is A Positive Regulator Of Gene Expression For The Cysteine Regulon, A System Of 10 Or More Loci Involved In The Biosynthesis Of L-Cysteine From Inorganic Sulfate. The Inducer For Cysb Is N-Acetylserine. Cysb Inhibits Its Own Transcription.

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=157, Percent_Identity=30.5732484076433, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=20.5479452054795, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786401, Length=250, Percent_Identity=27.2, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=115, Percent_Identity=33.0434782608696, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788296, Length=133, Percent_Identity=27.0676691729323, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=24.6575342465753, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=127, Percent_Identity=32.2834645669291, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=23.7154150197628, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=230, Percent_Identity=26.5217391304348, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=22.1311475409836, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=126, Percent_Identity=26.984126984127, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787589, Length=140, Percent_Identity=26.4285714285714, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787128, Length=148, Percent_Identity=23.6486486486486, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=152, Percent_Identity=26.9736842105263, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=24.2914979757085, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789506, Length=251, Percent_Identity=21.1155378486056, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34294; Mature: 34294

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRS
CCCHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEC
QKGLTITPEGEKVANYAKEMLQKEEEIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLK
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
KFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQEYKGQKQQLFEDELYLVDKEI
HHHHHCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHH
KDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
HHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCH
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK
HHHHHHHHHHHHHHHHCCHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRS
CCCHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEC
QKGLTITPEGEKVANYAKEMLQKEEEIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLK
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHH
KFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQEYKGQKQQLFEDELYLVDKEI
HHHHHCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHH
KDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
HHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCH
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSESARQLKQVQAFLEIIEEIQMEK
HHHHHHHHHHHHHHHHCCHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]