| Definition | Bacillus thuringiensis str. Al Hakam chromosome, complete genome. |
|---|---|
| Accession | NC_008600 |
| Length | 5,257,091 |
Click here to switch to the map view.
The map label for this gene is murE [H]
Identifier: 118479134
GI number: 118479134
Start: 3763688
End: 3765163
Strand: Reverse
Name: murE [H]
Synonym: BALH_3544
Alternate gene names: 118479134
Gene position: 3765163-3763688 (Counterclockwise)
Preceding gene: 118479135
Following gene: 118479133
Centisome position: 71.62
GC content: 37.53
Gene sequence:
>1476_bases ATGAAGTTGCATACACTTGTATCATGTTTGCATGATTTTCCAGTTGTTCCAAAAGAAAATCCAGAAATCACATCTATAGA AGCTGATTCACGTAAAGTGAAAGAGGGAAGCTTATTTGTATGTATGAAAGGGTATACGGTTGATAGCCATGATTTTGCTA AGCAAGCAGCGGCACAAGGAGCGGCTGCTATTGTTGCGGAAAGACCAATTGATGTTGACGTTCCAGTTGTACTTGTGAAA AACACTTTTCGTTCGTTAGCGGTTTTAGCTGATTATTTTTATGGTCAACCAACACACAAGTTACATTTAATCGGTATTAC AGGTACAAATGGAAAGACAACAACATCGCATATTATGGATGAGATTATGCGCGCACATGGTCATAAAACGGGGCTAATTG GAACGATTAATATGAAAATCGGTGATGAAACATTTGAGGTGAAAAATACAACGCCAGATGCACTAACACTTCAACAGACG TTTGCAAAAATGGTTGAACACGGTGTGGATAGCACAGTAATGGAAGTTTCATCACATGCTTTAGATCTTGGTCGTGTACA CGGATGTGATTACGATGTAGCGGTGTTTACAAATTTAACACAAGATCATTTAGACTACCATAAAACGATGGAAGAATATA AACATGCAAAAGGGTTGCTATTTGCACAACTTGGCAATAGTTATAATCATAATCGTGAAAAATACGCGGTACTGAATAAC GATGATGCTGTAACAGCGGAATATATGAGAAGTACTGCAGCAACTGTTGTAACGTATGGAATTGATACAACAAGTGATGT TATGGCCAAAGATATCGTTATGACAAGTGGTGGAACTACATTTACGCTTGTAACACCGTATGAAAGTGTAAATGTTACGA TGAAATTAATTGGGAAATTTAATGTATATAACGTACTAGCTGCAACAGCGGCAGGACTTGTTTCTGGTGTAAGTTTGGAA ACAATCATTGATGTAATAAAGGAACTAGCTGGAGTTCCTGGACGTTTCGAAGTAGTTGATGGTGGCCAAAATTATACAGT TATTGTTGATTATGCACATACACCAGATAGCTTAGAAAATGTATTGAAAACAGCAAAGCAGTTTGCAAAAGGTGACGTAT ATTGCATCGTCGGTTGTGGTGGTGATAGAGATCGAACGAAGAGACCAATCATGGCAAGTGTTGCCACACAATATGCAACT CACGCAATTTACACTTCAGATAATCCAAGAAGTGAAGATCCAGCGGCTATTTTAGATGATATGGTACATGGTGCAAATGG AGATAATTATGAAGTGATTATTGATAGAAAAGAAGCAATACACCATGCGATCACTAAAGCGAAAGCAGATGATATCATTA TTATTGCTGGTAAGGGTCATGAAACATATCAAATTATTGGTAAAGATGTTCATCATTTTGACGATCGTGAAGTCGCTAAA GAAGCAATTACAGAGCGTTTAAACAACGAAGAGTAA
Upstream 100 bases:
>100_bases AGACACAGTAGCGCTTACCTCCTATTTTACATAATTAGGAGGTAGCGTGTTGCTTATTAATAAAAGAGATAAATACACAA TGTAGAGAGGGTTTAGTGAA
Downstream 100 bases:
>100_bases CAACGTGAGAGGAGGACTACATGTGCTTGAGCAAGGTTTATTAGTAACGGCTGGGGTAGCATTCTTAATTTCTGTTGCCC TTTCGCCATTGTTTATTCCA
Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
Products: NA
Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]
Number of amino acids: Translated: 491; Mature: 491
Protein sequence:
>491_residues MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYAT HAIYTSDNPRSEDPAAILDDMVHGANGDNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK EAITERLNNEE
Sequences:
>Translated_491_residues MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYAT HAIYTSDNPRSEDPAAILDDMVHGANGDNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK EAITERLNNEE >Mature_491_residues MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYAT HAIYTSDNPRSEDPAAILDDMVHGANGDNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK EAITERLNNEE
Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]
COG id: COG0769
COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family. MurE subfamily [H]
Homologues:
Organism=Escherichia coli, GI1786273, Length=480, Percent_Identity=35.625, Blast_Score=268, Evalue=4e-73, Organism=Escherichia coli, GI1786274, Length=339, Percent_Identity=28.9085545722714, Blast_Score=110, Evalue=2e-25,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005761 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.13 [H]
Molecular weight: Translated: 53706; Mature: 53706
Theoretical pI: Translated: 5.72; Mature: 5.72
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQG CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEECCHHHHHHHHHCC AAAIVAERPIDVDVPVVLVKNTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMD CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH EIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQTFAKMVEHGVDSTVMEVSSHA HHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH LDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECC DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKF CCCHHHHHHHHHHHEEEEECCCCCHHHHHHHEEEECCCCEEEEECCCCCCCEEEEEEECC NVYNVLAATAAGLVSGVSLETIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLEN CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH VLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYATHAIYTSDNPRSEDPAAILDD HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH MVHGANGDNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK HHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCHHHHHH EAITERLNNEE HHHHHHHCCCC >Mature Secondary Structure MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQG CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEECCHHHHHHHHHCC AAAIVAERPIDVDVPVVLVKNTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMD CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH EIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQTFAKMVEHGVDSTVMEVSSHA HHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH LDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECC DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKF CCCHHHHHHHHHHHEEEEECCCCCHHHHHHHEEEECCCCEEEEECCCCCCCEEEEEEECC NVYNVLAATAAGLVSGVSLETIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLEN CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH VLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYATHAIYTSDNPRSEDPAAILDD HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH MVHGANGDNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK HHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCHHHHHH EAITERLNNEE HHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12721629 [H]