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The map label for this gene is alkA [H]

Identifier: 118478964

GI number: 118478964

Start: 3570346

End: 3571257

Strand: Direct

Name: alkA [H]

Synonym: BALH_3370

Alternate gene names: 118478964

Gene position: 3570346-3571257 (Clockwise)

Preceding gene: 118478963

Following gene: 118478966

Centisome position: 67.91

GC content: 33.77

Gene sequence:

>912_bases
ATGACAGCCGAATATAGTAACGCATTAACTTTAACAGTTCCAACAGAATTTAGTTTTCATGAAAATTTACGCTATCTATC
CCGTTCTAACAATGAATGTATGTTCCACATTGAAGATAACAAAATTTATAAAGTTATCCCTATTCACGATGTAAATCCTT
TAGTTGAAATTAGTATGAATGCGGATGATACTATTCAAATACGTTTTTTAGGAGAAGCCTATATCTCTGCAAAACCGATA
CGCGATGCTGTAGCTAACTATGTAACTGAATGGTTTGATTTAACTACTGATTTAGCTCCCTTTTATACATTAGCTAAACA
TGATGTACTCCTTCAAAGACCAATTGAACAATATTATGGCCTTCGTACTTTAGGTATTCCAGACTTATTTGAGGCACTTT
CCTGGGGAATTATCGGGCAACAAATTAACCTCACATATGCTTATACACTAAAAAGAAGATTAGTCGAGACATTTGGAAGT
TATGTAGAATGGAACGGTAGAAAACATTGGATATTCCCTTCTCCTGAAACGATTGCCAATTTACACGTAGAGGATCTCAA
AAATTTAAAAATGACGACTAGAAAATGTGATTATTTAATCGGTATTGCAAAGCTTATTACAGAAGGAAAATTATCAAAAG
AATCTTTACTGCAAATACAAGACGTGAAACAAGCTGAAAAAAGATTAACTGCTATACATGGTATAGGACCGTGGACGGCC
AACTATGTTTTAATGCGTTGTTTACGATTTCCTTCTGCTTTTCCAATTGACGATGTTGGTTTGCATAATGCAATTAAATA
TTTAACAGGTTCTGAAAGTAAACCGACTAAACATGAAATCAAAGATTTTGCGGTAAACTGGAAAAATTGGGAGTCTTATG
CGACTTTTTACCTATGGCGAGTATTATACTAA

Upstream 100 bases:

>100_bases
ACCGTGTTATTGGAAAGAATGGCAAACTAACTGGTTTTAGAGGGGGATTAGAAATGAAGAAAGAATTGCTTGTATTAGAA
AAATTACAGGTGGAATTCAT

Downstream 100 bases:

>100_bases
AAACCAAGTAGCACATCTGCACTACTTGGTTCTTTTTTAAGCTTGCTCTTCAAATCGTCTTGCAATCTCTATAATAACTT
GAACAGCTTTTTCCATAACA

Product: DNA-3-methyladenine glycosylase II

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTAEYSNALTLTVPTEFSFHENLRYLSRSNNECMFHIEDNKIYKVIPIHDVNPLVEISMNADDTIQIRFLGEAYISAKPI
RDAVANYVTEWFDLTTDLAPFYTLAKHDVLLQRPIEQYYGLRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGS
YVEWNGRKHWIFPSPETIANLHVEDLKNLKMTTRKCDYLIGIAKLITEGKLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWRVLY

Sequences:

>Translated_303_residues
MTAEYSNALTLTVPTEFSFHENLRYLSRSNNECMFHIEDNKIYKVIPIHDVNPLVEISMNADDTIQIRFLGEAYISAKPI
RDAVANYVTEWFDLTTDLAPFYTLAKHDVLLQRPIEQYYGLRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGS
YVEWNGRKHWIFPSPETIANLHVEDLKNLKMTTRKCDYLIGIAKLITEGKLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWRVLY
>Mature_302_residues
TAEYSNALTLTVPTEFSFHENLRYLSRSNNECMFHIEDNKIYKVIPIHDVNPLVEISMNADDTIQIRFLGEAYISAKPIR
DAVANYVTEWFDLTTDLAPFYTLAKHDVLLQRPIEQYYGLRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGSY
VEWNGRKHWIFPSPETIANLHVEDLKNLKMTTRKCDYLIGIAKLITEGKLSKESLLQIQDVKQAEKRLTAIHGIGPWTAN
YVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWRVLY

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=180, Percent_Identity=31.1111111111111, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 35183; Mature: 35052

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEYSNALTLTVPTEFSFHENLRYLSRSNNECMFHIEDNKIYKVIPIHDVNPLVEISMN
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEC
ADDTIQIRFLGEAYISAKPIRDAVANYVTEWFDLTTDLAPFYTLAKHDVLLQRPIEQYYG
CCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGSYVEWNGRKHWIFPSPETIAN
EEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHEEECCEEEEECCCCHHHHH
LHVEDLKNLKMTTRKCDYLIGIAKLITEGKLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
VLY
HCC
>Mature Secondary Structure 
TAEYSNALTLTVPTEFSFHENLRYLSRSNNECMFHIEDNKIYKVIPIHDVNPLVEISMN
CCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEC
ADDTIQIRFLGEAYISAKPIRDAVANYVTEWFDLTTDLAPFYTLAKHDVLLQRPIEQYYG
CCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LRTLGIPDLFEALSWGIIGQQINLTYAYTLKRRLVETFGSYVEWNGRKHWIFPSPETIAN
EEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHEEECCEEEEECCCCHHHHH
LHVEDLKNLKMTTRKCDYLIGIAKLITEGKLSKESLLQIQDVKQAEKRLTAIHGIGPWTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NYVLMRCLRFPSAFPIDDVGLHNAIKYLTGSESKPTKHEIKDFAVNWKNWESYATFYLWR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
VLY
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]