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The map label for this gene is miaA

Identifier: 118478937

GI number: 118478937

Start: 3544251

End: 3545213

Strand: Reverse

Name: miaA

Synonym: BALH_3341

Alternate gene names: 118478937

Gene position: 3545213-3544251 (Counterclockwise)

Preceding gene: 118478939

Following gene: 118478936

Centisome position: 67.44

GC content: 37.07

Gene sequence:

>963_bases
TTGAATGATATGGGAGAAGTGCAACGTGAAAAAGTTGCTGTCATCATTGGACCAACTGCTGTTGGGAAGACGAAGTTAAG
TATTGATCTTGCTAAAGCGTTGAACGGTGAAATTATAAGTGGAGATTCCATGCAGATTTATCGTACGATGGATATTGGAA
CTGCAAAGGTGACGAAAGAAGAGATGGATGGAATTCCACATTATATGGTCGATATAAAAAATCCGGAAGAATCATTTTCA
GTTGCCGAGTTTCAAGAACGTGTTCGTAAGCATATTCGAGAGATTACAGAGCGTGGTAAATTACCAATTATCGTTGGTGG
AACTGGTCTGTATATACAGTCTGTTTTATTCGATTATCAGTTTACGGATGATGCTGGTGATGCTATATACCGAGAACAAA
TGGAAAAGTTAGCATTAGAACGTGGTGTGGAATATGTACATAAGAAATTACAAGAAGTAGACCCAGAAAGCGCTGAGCGT
ATTCATGCAAATAATGTGCGTCGTGTCATTAGAGCGTTAGAAATTTTTCACACGTCGGGTGAAAAAATGAGTGATCAACT
TGAAAAACAAGAAAACGAGTTATTATACGATGTTTCCTTAATTGGCTTGACAATGGATCGTGAAATGCTATACGATCGCA
TTAACTTACGAGTTGATATAATGATGGACCAAGGTTTATTAGAAGAAGTAGAAGGGTTGTATAATAGAGGGATACGAGAT
TGTCAATCTATTCAAGCGATTGGGTATAAAGAGATATATGATTATTTTGAGGATCGTGTCTCTTTAGAAGAAGCGGTATC
ACAATTAAAGACGAATTCACGTCGTTATGCAAAACGTCAATTAACGTGGTTCCGTAATAAGATGGATGTCACATGGTTTG
ATGTTACAGACGGTGAAAAAACGTCAGAAATTTTACGATACATAGAAGGAAAGCTACAACTAAAGTCGAATAATAGTAAG
TAG

Upstream 100 bases:

>100_bases
TTGTAATTTCATGTACCGTAATGAAAGAACTTGCAAACATGCCGTTAAAATTGGTAAGATTGGATATAATCAATAGATGG
AATACATTACATAAAGAGAG

Downstream 100 bases:

>100_bases
AGAAAAAGAGGAGGATTCGACATGAAGCAATCAATCAATATTCAAGATCAGTTTTTAAATCAACTCCGTAAAGAGAATAC
GTTTGTTACGCTGTACTTAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNDMGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFS
VAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAER
IHANNVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRD
CQSIQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Sequences:

>Translated_320_residues
MNDMGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFS
VAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAER
IHANNVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRD
CQSIQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK
>Mature_320_residues
MNDMGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFS
VAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAER
IHANNVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRD
CQSIQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=31.8471337579618, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1790613, Length=312, Percent_Identity=37.8205128205128, Blast_Score=220, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI25144712, Length=307, Percent_Identity=32.5732899022801, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=34.2019543973941, Blast_Score=179, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24649830, Length=341, Percent_Identity=31.3782991202346, Blast_Score=167, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BACAH (A0RH88)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_896088.1
- ProteinModelPortal:   A0RH88
- SMR:   A0RH88
- STRING:   A0RH88
- EnsemblBacteria:   EBBACT00000069290
- GeneID:   4544978
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_3341
- eggNOG:   COG0324
- GeneTree:   EBGT00050000001497
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   BTHU412694:BALH_3341-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 37013; Mature: 37013

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKE
CCCHHHHHHHCEEEEECCCCCCCEEHHHHHHHHCCCCEECCCCEEEEEEECCCCHHCCHH
EMDGIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQ
HHCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCE
FTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRD
HHHHHHHHHHHHHEEEEHHHHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHH
CQSIQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
TSEILRYIEGKLQLKSNNSK
HHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure
MNDMGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKE
CCCHHHHHHHCEEEEECCCCCCCEEHHHHHHHHCCCCEECCCCEEEEEEECCCCHHCCHH
EMDGIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQ
HHCCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCE
FTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRD
HHHHHHHHHHHHHEEEEHHHHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHH
CQSIQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
TSEILRYIEGKLQLKSNNSK
HHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA