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The map label for this gene is lexA

Identifier: 118478924

GI number: 118478924

Start: 3529309

End: 3529929

Strand: Direct

Name: lexA

Synonym: BALH_3328

Alternate gene names: 118478924

Gene position: 3529309-3529929 (Clockwise)

Preceding gene: 118478921

Following gene: 118478931

Centisome position: 67.13

GC content: 39.29

Gene sequence:

>621_bases
ATGGAAAAGTTAACGAAACGCCAGCAAGACATTCTCGACTTTATTAAGCTAAAAGTACAAGAAAAAGGATATCCACCTTC
CGTACGTGAAATCGGTCAAGCAGTCGGCCTCGCTTCTAGTTCTACAGTGCACGGACATTTATCAAGATTAGAAGAAAAAG
GATATATTCGACGCGATCCAACAAAACCACGTGCAATTGAAATTTTAGGTGAAGACCGAATGGATACAGAAACACAATCT
GTTATTCAAGTTCCAATTGTCGGAAAAGTTACTGCCGGTTTACCAATTACAGCGGTCGAAAGCGTAGAGGAGCACTTCCC
TCTTCCAGCTAGCATTGTCGCAGGAGCAGATCAAGTGTTTATGTTACGTATTTCCGGGGATAGTATGATTGAGGCTGGCA
TTTTCGATGGAGATTTAGTTGTTGTTCGCCAACAACAGTCTGCATATAATGGTGAAATTGTAGTCGCTTTAACAGAAGAT
AACGAAGCAACTGTTAAACGTTTCTATAAAGAAAAAGACCATTTCCGTCTACAACCGGAAAACTCTTCATTAGAACCTAT
CATTTTAAAACAAGTGTCAGTTATTGGTAAAGTAATTGGCGTATATCGTGATTTACATTAA

Upstream 100 bases:

>100_bases
CATAAATTCGCCTTTTGTTCGAACTTATGTTTGTAGAATGTTTTTTCACATGTTATACTTATTAATAAGAAAAATGAAAG
AAAACGAGGTGTTAGAAAAC

Downstream 100 bases:

>100_bases
AAGTAATAAAAAAAGATAATTACCTTATTTATATACGGTAATTATCTTTTTTGTATTTTGATAGAATTCCATTCATTCAA
AAGAATGCATTGCATTAATT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDPTKPRAIEILGEDRMDTETQS
VIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTED
NEATVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH

Sequences:

>Translated_206_residues
MEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDPTKPRAIEILGEDRMDTETQS
VIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTED
NEATVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH
>Mature_206_residues
MEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDPTKPRAIEILGEDRMDTETQS
VIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTED
NEATVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=209, Percent_Identity=38.2775119617225, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787431, Length=117, Percent_Identity=35.042735042735, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_BACAH (A0RH75)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_896075.1
- ProteinModelPortal:   A0RH75
- SMR:   A0RH75
- STRING:   A0RH75
- EnsemblBacteria:   EBBACT00000067749
- GeneID:   4543314
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_3328
- eggNOG:   COG1974
- GeneTree:   EBGT00050000002279
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   BTHU412694:BALH_3328-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22891; Mature: 22891

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: ACT_SITE 128-128 ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDP
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCC
TKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVF
CCCCEEEEECCCCCCCCCHHEEEECCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCEEE
MLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEATVKRFYKEKDHFRLQPE
EEEECCCCEEECCCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECCC
NSSLEPIILKQVSVIGKVIGVYRDLH
CCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDP
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCC
TKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVF
CCCCEEEEECCCCCCCCCHHEEEECCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCEEE
MLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEATVKRFYKEKDHFRLQPE
EEEECCCCEEECCCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECCC
NSSLEPIILKQVSVIGKVIGVYRDLH
CCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA