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The map label for this gene is dnaE [H]

Identifier: 118478852

GI number: 118478852

Start: 3447336

End: 3448283

Strand: Reverse

Name: dnaE [H]

Synonym: BALH_3250

Alternate gene names: 118478852

Gene position: 3448283-3447336 (Counterclockwise)

Preceding gene: 118478853

Following gene: 118478851

Centisome position: 65.59

GC content: 33.44

Gene sequence:

>948_bases
GTGGGGAATATATCCTTACCGTTAGATTATGTTGTAATTGACTTTGAAACAACAGGATTTAACCCTTATAATGATAAAAT
CATTCAAGTGGCGGCAGTGAAATATCGTAATCATGAACTAGTAGATCAATTTGTTTCCTATGTGAATCCGGAGCGTCCAA
TCCCGGACCGAATAACGAGTTTAACAGGTATTACAAATTATCGTGTTTCAGATGCGCCGACGATTGAAGAAGTGTTACCT
TTATTTTTAGCATTTTTACATACGAATGTAATTGTCGCTCATAACGCTTCTTTTGATATGCGCTTTTTGAAAAGCAACGT
CAATATGCTTGGGTTACCGGAACCTCAAAATAAAGTGATTGATACTGTACTTTTAGCAAAGAAATATATGAAGCACGCAC
CTAATCATAAACTTGAAACGTTAAAGAGAATGCTTGGAATTCGTTTAAGTTCTCATAATGCATTTGATGATTGTATTACG
TGTGCAGCTGTTTATCAAAAATGTGCATCTATTGAAGAAGAGGCAAAAAGGAAATTGAATAAGAAAGCACTTGATGAAAC
AGTAGTATATGAAGCGGTAAAAGACATACTTGTACGAAATAAACGTGATATCGAATGGATTCGTTGCATGAATGTAGGGA
GCTATTTAGATATAAAAGCTTTTTATCCAGTTATGAGATTAAAGGTAAAAGGGCGAAAGAAATATGTACTAACAGACATA
TTAGAAGATGATGTGAAAGAAATATGTACGAGTTTAGCATGTGAACCGGCATTAAAAAGTGAAGTTGGTAATACGAGAAT
TATGCTGAATAGCTTAGAGGATGTCTTGAAATTAGAAAGTTATATTTTAGAACAGTATGATTTCGTATTGCAAGCGCTGC
GTGATTATAAACAAAGTGAAATGAATGCAGATGAAAAGTTAAAAGAGTATTTAAATATTATGGTATAA

Upstream 100 bases:

>100_bases
GGTGGAAAGTAGCCATTTCTGCCTTTTCTTTTTCACGTAGTGATAGCAGCATGTTATGATAAAAGAAATATAAACGTTTA
GAGATAAAGGAGGGGTTCAA

Downstream 100 bases:

>100_bases
AAAACTCCGTGTACGATTTATTGTACACGGAGTTTTTTTAGGAGTACTAAAACATAAAATTTTCTTATAAATCTGATTAA
AGAGTTTTTCCTTTGCTCAT

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MGNISLPLDYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITSLTGITNYRVSDAPTIEEVLP
LFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPQNKVIDTVLLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCIT
CAAVYQKCASIEEEAKRKLNKKALDETVVYEAVKDILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI
LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSEMNADEKLKEYLNIMV

Sequences:

>Translated_315_residues
MGNISLPLDYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITSLTGITNYRVSDAPTIEEVLP
LFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPQNKVIDTVLLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCIT
CAAVYQKCASIEEEAKRKLNKKALDETVVYEAVKDILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI
LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSEMNADEKLKEYLNIMV
>Mature_314_residues
GNISLPLDYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITSLTGITNYRVSDAPTIEEVLPL
FLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPQNKVIDTVLLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCITC
AAVYQKCASIEEEAKRKLNKKALDETVVYEAVKDILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDIL
EDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSEMNADEKLKEYLNIMV

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2176

COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788149, Length=233, Percent_Identity=26.1802575107296, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786409, Length=173, Percent_Identity=28.9017341040462, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36238; Mature: 36106

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNISLPLDYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITS
CCCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
LTGITNYRVSDAPTIEEVLPLFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPQNKVI
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHHHCCCCEECCCCCHHHHH
DTVLLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCITCAAVYQKCASIEEEAKRKLN
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKALDETVVYEAVKDILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHHEECCCCHHHHHHH
LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSE
HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNADEKLKEYLNIMV
CCHHHHHHHHHHHCC
>Mature Secondary Structure 
GNISLPLDYVVIDFETTGFNPYNDKIIQVAAVKYRNHELVDQFVSYVNPERPIPDRITS
CCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
LTGITNYRVSDAPTIEEVLPLFLAFLHTNVIVAHNASFDMRFLKSNVNMLGLPEPQNKVI
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHHHCCCCEECCCCCHHHHH
DTVLLAKKYMKHAPNHKLETLKRMLGIRLSSHNAFDDCITCAAVYQKCASIEEEAKRKLN
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKALDETVVYEAVKDILVRNKRDIEWIRCMNVGSYLDIKAFYPVMRLKVKGRKKYVLTDI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHHEECCCCHHHHHHH
LEDDVKEICTSLACEPALKSEVGNTRIMLNSLEDVLKLESYILEQYDFVLQALRDYKQSE
HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNADEKLKEYLNIMV
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]