Click here to switch to the map view.

The map label for this gene is yfkO [H]

Identifier: 118478624

GI number: 118478624

Start: 3197125

End: 3197874

Strand: Direct

Name: yfkO [H]

Synonym: BALH_3003

Alternate gene names: 118478624

Gene position: 3197125-3197874 (Clockwise)

Preceding gene: 118478623

Following gene: 118478625

Centisome position: 60.82

GC content: 31.47

Gene sequence:

>750_bases
TTGTTTCTATGTTACTTTAAAAATATAATTTGGTTACAAAATACATTAATGAAAAGAGGAACTTTTATGACAAATACAAA
TCAAATTACAAAAGAAAAAATTATGGAGGCTTTTCATTTCAGACATGCATGTAAAGAGTTCGATTCTATGCAAAAAATTT
CAGAAGAGGATTTTAAATTTATTTTAGAAACAGGAAGATTATCTCCTTCTTCTTTCGGATATGAACCTTGGAAATTTGTT
GTCGTACAAAATAAAGAAGTAAGAGAAAAACTACGACCATATTCATGGGGCGCTGGTGGGCAACTTGCAACAGCTAGCCA
CTTTGTCATTGTTCTTTCAAGAAATATTAAAGATATGCATTATGATGCAGAGTATATTAAACATATGATGAATGATATTA
TTGAATTACCTGAAGACACGCAAAAAATTAGATATGAATTCTTCAAAAAGTTCCAGGAAACAGATTTTAACTTATTACAA
TCCGACCGTGCTGTATTTGATTGGGCATCAAAGCAAACTTATATTGCTTTAGGTAATATGATGACGAGTGCAGCTCAAAT
TGGCATTGATTCTTGTCCAATAGAAGGATTTGATAAAGAGAAAGTAGATTCTTTACTTCGTCAAGAAGGCATAATCAATG
GCGATAACTTTGAAGTATCTGTTATGGTTGCCTTTGGTTACCGTAAAGAAGAGCCAAAGCGTGATAAAACAAGACAAACT
ATGGATACAATTGTTGAATGGATTTATTAA

Upstream 100 bases:

>100_bases
AACGGGTAATGTATATTATTTTCGTTTGAACAATCCATAATAAATCCCTTCCCTTAACATTAGTATCTTTTTTGATACTA
TACCACATAAATGTGCGTAC

Downstream 100 bases:

>100_bases
GATAACACGCGCTCCTTATAGCTGCATACCCATACGAAAGAAGTAGAATTTCATTTTTCTTATGTATGAAATTGATTTTT
CATGGAAAACTATATTAGTA

Product: nitroreductase family protein

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MFLCYFKNIIWLQNTLMKRGTFMTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFV
VVQNKEVREKLRPYSWGAGGQLATASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDTQKIRYEFFKKFQETDFNLLQ
SDRAVFDWASKQTYIALGNMMTSAAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQT
MDTIVEWIY

Sequences:

>Translated_249_residues
MFLCYFKNIIWLQNTLMKRGTFMTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFV
VVQNKEVREKLRPYSWGAGGQLATASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDTQKIRYEFFKKFQETDFNLLQ
SDRAVFDWASKQTYIALGNMMTSAAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQT
MDTIVEWIY
>Mature_249_residues
MFLCYFKNIIWLQNTLMKRGTFMTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFV
VVQNKEVREKLRPYSWGAGGQLATASHFVIVLSRNIKDMHYDAEYIKHMMNDIIELPEDTQKIRYEFFKKFQETDFNLLQ
SDRAVFDWASKQTYIALGNMMTSAAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQT
MDTIVEWIY

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=174, Percent_Identity=29.3103448275862, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 29372; Mature: 29372

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLCYFKNIIWLQNTLMKRGTFMTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKF
CEEHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILETGRLSPSSFGYEPWKFVVVQNKEVREKLRPYSWGAGGQLATASHFVIVLSRNIKDMH
HHHHCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHH
YDAEYIKHMMNDIIELPEDTQKIRYEFFKKFQETDFNLLQSDRAVFDWASKQTYIALGNM
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHCCCCCEEEHHHH
MTSAAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQT
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHH
MDTIVEWIY
HHHHHHHCC
>Mature Secondary Structure
MFLCYFKNIIWLQNTLMKRGTFMTNTNQITKEKIMEAFHFRHACKEFDSMQKISEEDFKF
CEEHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILETGRLSPSSFGYEPWKFVVVQNKEVREKLRPYSWGAGGQLATASHFVIVLSRNIKDMH
HHHHCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHH
YDAEYIKHMMNDIIELPEDTQKIRYEFFKKFQETDFNLLQSDRAVFDWASKQTYIALGNM
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHCCCCCEEEHHHH
MTSAAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQT
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHH
MDTIVEWIY
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]