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The map label for this gene is yjfC [H]

Identifier: 118478261

GI number: 118478261

Start: 2777145

End: 2778392

Strand: Reverse

Name: yjfC [H]

Synonym: BALH_2622

Alternate gene names: 118478261

Gene position: 2778392-2777145 (Counterclockwise)

Preceding gene: 118478262

Following gene: 118478259

Centisome position: 52.85

GC content: 34.62

Gene sequence:

>1248_bases
ATGTATACAGAACAAGAACAGAAAGAGTATATGAAAGTATGGTTTTCACTTGCAGAAAAGGCTGGTCGGAATGGGTTTAC
TTGGCCGAGTCTATTAGAAAATGAAGAATGGAACCAATATATGGCAACAGGTATGTATCGTATGCCAATAAAAACTTATA
CTGCTATTTCAAAAGCGACAGAAGAAATTATGTATGTGCTATATAGAACGTATCAATACATTCTCAATACGACAAAAGAT
TTTCAAAAACTAGGATTTCCAGCTGAAACGTGGGAAATTGCGAGAATGAAACATACTGGTTTGTTTTCATATTTTACAAG
GTTTGACTTTATCGTAAATGGTGAAGATATAAAGTTAATAGAAGTAAATTGTGATACACCCACAGGTTATTTAGAGCCAT
CTGTCGCAAATGAAGTGTTATGCCGTTATCACGATGTAAATCACCCAAATCATATAGAAGAGCATATTGTGCAGGCGTGG
GAACAAATTAAACATGACTATAGTATAGATCCTAGAGAAAAGATTTATTTTACGAGTTATGATTGGCATGATGAAGACCA
TCAAACGGTTCAATTTTTAAGAAGCTATTGCTTAGATCAGTCAACGGATTATATAGGTATACAAGATATCGTCGTGGCAG
ATGATGGTATATATACGCAAAATGGTGAACGAATCCATTATTTATATAGACTATATCCAATTGAATATTTAGTATCAGAC
GCTGATAAAAACGGGAAAAGAATTGGACTCCAGTTTTTAGATCATATAGCGCAAGGTAGAGTGAAAATCATTAATCCACC
AGCTGCTTTTCTTATGCAAAATAAAAGTGTACTCGCATTAATATGGCAACTTTATGAAGAGGAAGTTTTCTTTGAGGAAG
AGGAGCGAGGAATCATTCAGAACTACTTCTTACCGACATATTTTACAAATAAACCGTTTATAAAAAGAAATGAATCATAT
GTTTCTAAACCTTTATACGGCCGTGAAGGTGGAGGAGTATCTATATACGAAAATAATGAACTATTAGCTGAAGATAAAAC
AGAGTATTACTTTGAACAGCGGAAAATATATCAGCAATACGTAGAAATGCCTGACTATACGATTGACACGTGGGACGGTC
CGTATACTGGTAAACTATTAATTGGTTCACACTGTATTAGCGGGAGAGCTGCTGGTTTATTTTTACGTGTAGGTGAGAAA
ATAACGGGAAACTTATCGATGTTTACTGGAGTTACAATTGAAGGATAA

Upstream 100 bases:

>100_bases
AATCATCTACGACACAAACGCCGAAAGTGAATTCTGGTTCTAGTGGAATTGGGAACGCAAAAGCGCCAGCAGGATCCTAA
AATGAAAGGATAATGGAGTA

Downstream 100 bases:

>100_bases
ATGAAACAAAGAGCTTATCATTTTTGATGAGCTCTTTGTTCATATAGCGTTTTTATTGCTGTGATGTAACGCTTTCATTT
TTCTTCATTTCAAAGATTTT

Product: glutathionylspermidine synthase

Products: N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MYTEQEQKEYMKVWFSLAEKAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKATEEIMYVLYRTYQYILNTTKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPREKIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTQNGERIHYLYRLYPIEYLVSD
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQNYFLPTYFTNKPFIKRNESY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYVEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG

Sequences:

>Translated_415_residues
MYTEQEQKEYMKVWFSLAEKAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKATEEIMYVLYRTYQYILNTTKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPREKIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTQNGERIHYLYRLYPIEYLVSD
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQNYFLPTYFTNKPFIKRNESY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYVEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG
>Mature_415_residues
MYTEQEQKEYMKVWFSLAEKAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKATEEIMYVLYRTYQYILNTTKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPREKIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTQNGERIHYLYRLYPIEYLVSD
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQNYFLPTYFTNKPFIKRNESY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYVEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=418, Percent_Identity=26.3157894736842, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789416, Length=404, Percent_Identity=23.5148514851485, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: 6.3.1.8

Molecular weight: Translated: 48874; Mature: 48874

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTEQEQKEYMKVWFSLAEKAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHHHHHHHHH
EEIMYVLYRTYQYILNTTKDFQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLI
HHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEE
EVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAWEQIKHDYSIDPREKIYFTSY
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
DWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTQNGERIHYLYRLYPIEYLVSD
CCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCCCEEEEEEEEHHHHHHHHC
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQ
CCCCCCEEHHHHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCHHH
NYFLPTYFTNKPFIKRNESYVSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQY
HCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHHH
VEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTIEG
HCCCCCEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MYTEQEQKEYMKVWFSLAEKAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHHHHHHHHH
EEIMYVLYRTYQYILNTTKDFQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNGEDIKLI
HHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEE
EVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAWEQIKHDYSIDPREKIYFTSY
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
DWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTQNGERIHYLYRLYPIEYLVSD
CCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCCCEEEEEEEEHHHHHHHHC
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQ
CCCCCCEEHHHHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCHHH
NYFLPTYFTNKPFIKRNESYVSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQY
HCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHHH
VEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTIEG
HCCCCCEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gamma-L-glutamyl-L-cysteinyl-glycine; spermidine; ATP

Specific reaction: gamma-L-glutamyl-L-cysteinyl-glycine + spermidine + ATP = N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]