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The map label for this gene is ilvB [H]

Identifier: 118477309

GI number: 118477309

Start: 1771266

End: 1772978

Strand: Direct

Name: ilvB [H]

Synonym: BALH_1624

Alternate gene names: 118477309

Gene position: 1771266-1772978 (Clockwise)

Preceding gene: 118477308

Following gene: 118477310

Centisome position: 33.69

GC content: 40.22

Gene sequence:

>1713_bases
ATGAAGCAATATACAACCTATGAGAAATTGCAGTGTGAAGAAGTGACAGGAGCTGGACACGTTATTCAATGCTTAAAGAA
ATTAGGCGTAACGACTGTTTTCGGTTATCCGGGCGGAGCAATTTTACCAGTGTACGATGCGCTATATGAAAGTGGATTGA
AGCATATATTAACTCGTCATGAACAAGCTGCTATTCATGCGGCTGAAGGTTATGCAAGAGCTTCTGGAAAGGTCGGGGTA
GTATTTGCTACCTCTGGCCCAGGGGCGACGAATTTAGTTACAGGATTAGCAGATGCTTATATGGATTCGATTCCTTTAGT
TGTCATTACAGGGCAAGTTGCAACACCACTCATTGGAAAAGACGGGTTTCAAGAAGCTGATGTTGTCGGAATTACAGTAC
CTGTTACGAAGCATAATTACCAAGTTCGTGATGTGAATCAGTTATCACGTATTGTACAAGAAGCTTTTTACATCGCCGAA
AGCGGGCGGCCAGGACCAGTATTAATTGATATTCCAAAAGATGTTCAAATTGAAAAGGTAACAAGTTTTTACAATGAAGT
AATTGAGATTCCAGGGTACAAAATAGAGGACATGCCAGACAGTATGAAGCTTAAAGAGGTAGCTAAAGAAATTTCAAAAG
CGAAACGCCCACTTCTTTATATTGGAGGAGGTGTCATTCATTCGGGTGGATCTGATGAACTCATCAAATTTGCGAGGGAG
CATCGTATTCCTGTCGTTTCCACCTTAATGGGACTGGGTGCATATCCGCCGGGAGATTCGTTATTTTTAGGAATGCTTGG
TATGCATGGAACGTACGCTGCAAATATGGCTGTAACAGAATGTGATCTACTACTTGCTTTAGGTGTTCGCTTCGATGATC
GTGTAACAGGAAAATTAGAACTTTTTTCTCCGCAGTCGAAAAAGGTACATATTGATATAGATTCTTCGGAGTTCCATAAA
AATGTAACTGTGGAATATCCGGTTGTTGGAGATGTGAAAAATGCGTTACATATGTTGTTACATATGCCGATTGATACACA
GACAGATGAATGGCTTACGAAAATCGAGGGATGGAAAGAGGAATACCCTCTTTCCTACAACCAAAAAGAAAGGGAGTTAA
AACCACAGCATGTTATTAGTCTAGTAAGCGAATTAACGAATGGTGAAGCAATTGTTACGACAGAAGTAGGACAGCATCAA
ATGTGGGCTGCGCATTTTTATAAAGCGAAAAATCCACGGACTTTTCTCACATCTGGCGGACTAGGAACGATGGGGTTTGG
TTTTCCGGCGGCAATTGGTGCTCAGCTTGCGAAAGAAGAACAACTCGTCATTTGTATTGCAGGCGATGCTTCTTTTCAAA
TGAACATTCAAGAACTACAAACAGTTGCTGAAAATAATATTCCTGTAAAAGTATTTATTATAAATAACAAATTTTTAGGG
ATGGTAAGGCAATGGCAAGAGATGTTTTATGAAAATCGTTTATCAGAATCAAAAATTGGATCTCCAGATTTTGTGAAAGT
GGCAGAAGCTTATGGAGTGAAGGGATTAAGGGCGACAAATTCAACAGAGGCGAAACAAGTGATGTTAGAAGCATTTGCTC
ACGAAGGTCCTGTTGTAGTTGATTTTTGTGTAGAAGAAGGTGAAAACGTATTTCCGATGGTTCCGCCAAACAAAGGGAAT
AACGAAATGATTATGAAGAGGTGGGAAGAATGA

Upstream 100 bases:

>100_bases
CAATTGACTGAGGAATTTAAAAAGTTAACGAGAGAAAGAGGCGTACGTGTTCCAGGGCTGGCAGAAAGCTTACTGTAGGT
AGGGAGAGGAGTTAATTGAA

Downstream 100 bases:

>100_bases
GTCATACTTTTTCACTCGTTATTCATAACGAGCCAAGCGTCTTATTACGTATAAGTGGAATTTTTGCTCGGCGTGGCTAT
TATATTTCTTCTTTACATTT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRHEQAAIHAAEGYARASGKVGV
VFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAE
SGRPGPVLIDIPKDVQIEKVTSFYNEVIEIPGYKIEDMPDSMKLKEVAKEISKAKRPLLYIGGGVIHSGGSDELIKFARE
HRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPQSKKVHIDIDSSEFHK
NVTVEYPVVGDVKNALHMLLHMPIDTQTDEWLTKIEGWKEEYPLSYNQKERELKPQHVISLVSELTNGEAIVTTEVGQHQ
MWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAIGAQLAKEEQLVICIAGDASFQMNIQELQTVAENNIPVKVFIINNKFLG
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPNKGN
NEMIMKRWEE

Sequences:

>Translated_570_residues
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRHEQAAIHAAEGYARASGKVGV
VFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAE
SGRPGPVLIDIPKDVQIEKVTSFYNEVIEIPGYKIEDMPDSMKLKEVAKEISKAKRPLLYIGGGVIHSGGSDELIKFARE
HRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPQSKKVHIDIDSSEFHK
NVTVEYPVVGDVKNALHMLLHMPIDTQTDEWLTKIEGWKEEYPLSYNQKERELKPQHVISLVSELTNGEAIVTTEVGQHQ
MWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAIGAQLAKEEQLVICIAGDASFQMNIQELQTVAENNIPVKVFIINNKFLG
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPNKGN
NEMIMKRWEE
>Mature_570_residues
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRHEQAAIHAAEGYARASGKVGV
VFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGKDGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAE
SGRPGPVLIDIPKDVQIEKVTSFYNEVIEIPGYKIEDMPDSMKLKEVAKEISKAKRPLLYIGGGVIHSGGSDELIKFARE
HRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLELFSPQSKKVHIDIDSSEFHK
NVTVEYPVVGDVKNALHMLLHMPIDTQTDEWLTKIEGWKEEYPLSYNQKERELKPQHVISLVSELTNGEAIVTTEVGQHQ
MWAAHFYKAKNPRTFLTSGGLGTMGFGFPAAIGAQLAKEEQLVICIAGDASFQMNIQELQTVAENNIPVKVFIINNKFLG
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVVDFCVEEGENVFPMVPPNKGN
NEMIMKRWEE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=585, Percent_Identity=25.2991452991453, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=24.8214285714286, Blast_Score=159, Evalue=9e-39,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=45.7242582897033, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=42.6548672566372, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=34.2960288808664, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=26.9230769230769, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=22.9537366548043, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=26.5709156193896, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=26.5709156193896, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=588, Percent_Identity=25.3401360544218, Blast_Score=152, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=45.3913043478261, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=26.9151138716356, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321524, Length=500, Percent_Identity=23.2, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=27.0325203252033, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62784; Mature: 62784

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRH
CCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
EQAAIHAAEGYARASGKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGK
HHHHHHHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAESGRPGPVLIDIPKDVQIEKV
CCCCCCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
TSFYNEVIEIPGYKIEDMPDSMKLKEVAKEISKAKRPLLYIGGGVIHSGGSDELIKFARE
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCCHHHHHHHHH
HRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLE
CCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEE
LFSPQSKKVHIDIDSSEFHKNVTVEYPVVGDVKNALHMLLHMPIDTQTDEWLTKIEGWKE
EECCCCCEEEEEECCHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHH
EYPLSYNQKERELKPQHVISLVSELTNGEAIVTTEVGQHQMWAAHFYKAKNPRTFLTSGG
CCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCC
LGTMGFGFPAAIGAQLAKEEQLVICIAGDASFQMNIQELQTVAENNIPVKVFIINNKFLG
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCHHHH
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEE
DFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEECCCCCEECCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKQYTTYEKLQCEEVTGAGHVIQCLKKLGVTTVFGYPGGAILPVYDALYESGLKHILTRH
CCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
EQAAIHAAEGYARASGKVGVVFATSGPGATNLVTGLADAYMDSIPLVVITGQVATPLIGK
HHHHHHHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DGFQEADVVGITVPVTKHNYQVRDVNQLSRIVQEAFYIAESGRPGPVLIDIPKDVQIEKV
CCCCCCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
TSFYNEVIEIPGYKIEDMPDSMKLKEVAKEISKAKRPLLYIGGGVIHSGGSDELIKFARE
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCCHHHHHHHHH
HRIPVVSTLMGLGAYPPGDSLFLGMLGMHGTYAANMAVTECDLLLALGVRFDDRVTGKLE
CCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEE
LFSPQSKKVHIDIDSSEFHKNVTVEYPVVGDVKNALHMLLHMPIDTQTDEWLTKIEGWKE
EECCCCCEEEEEECCHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHH
EYPLSYNQKERELKPQHVISLVSELTNGEAIVTTEVGQHQMWAAHFYKAKNPRTFLTSGG
CCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCC
LGTMGFGFPAAIGAQLAKEEQLVICIAGDASFQMNIQELQTVAENNIPVKVFIINNKFLG
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCHHHH
MVRQWQEMFYENRLSESKIGSPDFVKVAEAYGVKGLRATNSTEAKQVMLEAFAHEGPVVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEE
DFCVEEGENVFPMVPPNKGNNEMIMKRWEE
EEEECCCCCEECCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]