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The map label for this gene is cls [H]

Identifier: 118477268

GI number: 118477268

Start: 1720211

End: 1721428

Strand: Direct

Name: cls [H]

Synonym: BALH_1579

Alternate gene names: 118477268

Gene position: 1720211-1721428 (Clockwise)

Preceding gene: 118477267

Following gene: 118477270

Centisome position: 32.72

GC content: 32.18

Gene sequence:

>1218_bases
ATGAACATGATTAAAAAAATATTGCGAGTTACTTCCATTATTATTGCTATTTTCGCTTTTATTTTAATTTGTATGCATAT
TGATGTTACTTTAGGTAGGAAAATGGAAGCTGGGAAAAACATGCCGACAGAGTATGCACCTCATTATAGTGATTTTCAAT
TATATGTAGAAGGAAAGTCATTTTATAAACAGATATTTACTGATATAAAAGAAGCGAAACAATCTATTTACACTTATTTT
TTTATTTTATCGGATGATAAAAGTAGTCATACTTTTTTAAACTTATTAAAAGAAAAGGCAAGAGAAGGAGTAAACGTATA
TTTATCTGTCGATTTACTTAACGATTTATCATTTGAAAGAAAGATGAAAAAAGAATTACAGGAAAATGGTGTACATTTTA
CATATAGTAGAAAACAGGAATTACCATTCGGGTTTTATTCACTTCACCATCGTAATCATCGCCGCATTACAACGATTGAT
GGAGAGATTGGTTATACGGGTGGTTTTAATATAGGGGATGAATACTTAGGGAAAGATAAACACTTTGGGTACTGGAGAGA
CTATCATGTACGGATAAAAGGTGAAGGTGCAAAAGACTTAGAGGAACAATTTGCTTTAGATTGGAAACGAGATACGAAAG
AAGATATAAAGAGGAGTACGAATAAGGCTAGCAAAGGGAATACACTACATACTATGGTTAGTTATAATGGGCATCACGTC
GCTGAAAAATATATAGATCTTATAAAACAAGCTCAGCACTCAATTGTAATTGCAACGCCGTATTTTATAGCGAAAAATAA
AGAATCCATGAATGCTTTAATCGCAGCACAAAAGCGTGGTGTTACAGTAAAAATACTTTGGTCATATAAACCAGATCTAC
CTCTTATAAAAGAAGCGGCGTATCCATACATACGTCAAGCTGTTAATAATGGGATTACTGTATATGGGTATAAAAAAGGA
ATGTTCCATGGCAAATTAATGCTTATTGATAATGAATTAACCGTTATCGGCACAACAAACTTTACTTCGCGTAGTTTCAA
TATAAATGATGAAATGAATTTTTATATTCATGGAGGTCCTATTGTAGGGCAAGTTAATAAGGCGTTAACAGAGGATTTTC
GTGATTCAAAAGAAATGACGAAAGAATTTTTTGAGAAGTTATCTTTTTGGGAGCGTTGTAAGGAGAAATTTGCTGGGATG
GTGGATTTTTATTTATAA

Upstream 100 bases:

>100_bases
AAAGGGTGTGACATTAAGTCGCATCCTTTTTTGTATGAAGCATGAAATCTAAATTTGAATGCCAAAATAGTAGGGTGGAA
AGAAAAATGAGCGGAAGGAA

Downstream 100 bases:

>100_bases
AGGTTAATGGAAAAAGGGTGTCTTTTATAGACACCCTTTTAAAATGAGAAATAATTATTTCTCATGCGCAAGTAAAGAAG
CGTCATCATTAATCAATGTT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQIFTDIKEAKQSIYTYF
FILSDDKSSHTFLNLLKEKAREGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTID
GEIGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHHV
AEKYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAVNNGITVYGYKKG
MFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGM
VDFYL

Sequences:

>Translated_405_residues
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQIFTDIKEAKQSIYTYF
FILSDDKSSHTFLNLLKEKAREGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTID
GEIGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHHV
AEKYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAVNNGITVYGYKKG
MFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGM
VDFYL
>Mature_405_residues
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQIFTDIKEAKQSIYTYF
FILSDDKSSHTFLNLLKEKAREGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTID
GEIGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHHV
AEKYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAVNNGITVYGYKKG
MFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGM
VDFYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=359, Percent_Identity=25.3481894150418, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787007, Length=372, Percent_Identity=25.5376344086022, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47136; Mature: 47136

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCHH
FYKQIFTDIKEAKQSIYTYFFILSDDKSSHTFLNLLKEKAREGVNVYLSVDLLNDLSFER
HHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHH
KMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDK
HHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
HFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHHV
CCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHH
AEKYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAA
HHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
YPYIRQAVNNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGP
CHHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCEECCCCCEEEEEECCC
IVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGMVDFYL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MNMIKKILRVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCHH
FYKQIFTDIKEAKQSIYTYFFILSDDKSSHTFLNLLKEKAREGVNVYLSVDLLNDLSFER
HHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHH
KMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDK
HHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
HFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHHV
CCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHH
AEKYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAA
HHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
YPYIRQAVNNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGP
CHHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCEECCCCCEEEEEECCC
IVGQVNKALTEDFRDSKEMTKEFFEKLSFWERCKEKFAGMVDFYL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]