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The map label for this gene is dacB [H]

Identifier: 118477025

GI number: 118477025

Start: 1470193

End: 1471353

Strand: Direct

Name: dacB [H]

Synonym: BALH_1325

Alternate gene names: 118477025

Gene position: 1470193-1471353 (Clockwise)

Preceding gene: 118477024

Following gene: 118477026

Centisome position: 27.97

GC content: 36.69

Gene sequence:

>1161_bases
TTGTACAAAAAGTGCCAAAAGGACGTGGGGGGAAATATGAGACGAATTTGTGTAATCATAACGCTTCTTATTATTTATGC
AAGCGTTATGCCAATTCCTACATATGCAAAGATGAACAGCAATGTCAGTGCTCGTAATGCTGTATTAATGGAACAACATT
CTGGACGTGTATTATACGGAAAAGCAGAACATGAGCCGCAAAAAATTGCTAGTATAACAAAAATTATGACAGCCTTATTA
GCTGCTGAATCAGGAAAGATGAAAGAAAGGGTATTAGTAAGTAATGAAGCGGTCAGGGTCGAAGGATCTGCAATTTATTT
AAAGCCTGGGCAAAAAGTGAAGCTAGAGGATTTAGTATACGGACTTATGCTTAGATCTGGTAATGACGCAGCACAAGTAA
TTGCTGAAAATGTAGGAGGGAGTATAGAAGGGTTCGTATATTTAATGAATGAAAAGGCGAAACAGATTGGAATGAAAGAT
ACTCACTTTTCAAACCCACATGGTTTGGATGGAGATGGATCTCATTATTCGTCGGCCTATGACATGGCACTTTTAACGAG
ATATGCAATGGGGAACGAGACTTTTAAGAAAATTTTTGGAACAAAAACGTATAAATCAGATTCGTGGGATTATCCGTGGA
AAAACAAACATAAACTTGTGACGTCGTATTATGAATTTGCAACAGGGGGAAAAACAGGTTTTACGAAGAAAGCAGGAAGA
ACGCTTGTTACAACAGCATCAAAAGATGGACTAGATCTAATTGTCGTAACTTTGAGCGCTTCTAGTGATTGGGATGATCA
TATGAATTTATTTGATAAAGGTTTTGACCGTTTCGAACAAACAAAAGTTTTAGGACAAGGAGCACTTGCTGAAATAACCG
AAAAGAAATACGCCAATCATGTTTACACGAAAAATAGTTTTTCAGTACCTTTAACTGAAGAGGAAAAAAAGAGCGTAGTA
TTAAAAGTTGAACTAGATAAAAGTGCAAAGCTTGTGGATGGAGTAAAGGTTGGAAAGACAGACGTTTATGTTGGCAATGA
GAAAGTTGGAGAACGGAATTTATTTTATAGTAAACGAAAGTTAGTAGCTACAACCGGGATGTACTGGAATAATGTGAAAG
AAATTTTTTCTTACATGATAGGTGTTGGTAGCGATGGTTAA

Upstream 100 bases:

>100_bases
ACAAATTGAATGGACACCGTATTAGACGCAGGTTTTCTGCGTCTTTTTTATTGGAAGGAGAAGGAGATTTCGTTCATATT
TTAAAAGGCCAAGCATACAC

Downstream 100 bases:

>100_bases
TCTCGTATGGGTAGCGATGGCAGTCATAGGGATTGTATATGCGATGATAAATGGAACGATGGAAGCAATAAATAAAGCTG
TATTTGATGGAGCGAAAGAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MYKKCQKDVGGNMRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALL
AAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKD
THFSNPHGLDGDGSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGR
TLVTTASKDGLDLIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVV
LKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGSDG

Sequences:

>Translated_386_residues
MYKKCQKDVGGNMRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALL
AAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKD
THFSNPHGLDGDGSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGR
TLVTTASKDGLDLIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVV
LKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGSDG
>Mature_386_residues
MYKKCQKDVGGNMRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALL
AAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKD
THFSNPHGLDGDGSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGR
TLVTTASKDGLDLIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVV
LKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGSDG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=372, Percent_Identity=29.5698924731183, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787062, Length=294, Percent_Identity=34.0136054421769, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=33.206106870229, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082054, Length=211, Percent_Identity=26.0663507109005, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42878; Mature: 42878

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKKCQKDVGGNMRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYG
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCEEEEE
KAEHEPQKIASITKIMTALLAAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVY
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCEEEEECCEEEECCCCEEEHHHHHH
GLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAY
HHHHCCCCHHHHHHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
DMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGR
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCC
TLVTTASKDGLDLIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANH
EEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
VYTKNSFSVPLTEEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRK
EEECCCCCCCCCCCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCC
LVATTGMYWNNVKEIFSYMIGVGSDG
EEEECCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYKKCQKDVGGNMRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYG
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCEEEEE
KAEHEPQKIASITKIMTALLAAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVY
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCEEEEECCEEEECCCCEEEHHHHHH
GLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAY
HHHHCCCCHHHHHHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
DMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGR
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCC
TLVTTASKDGLDLIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANH
EEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
VYTKNSFSVPLTEEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRK
EEECCCCCCCCCCCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCC
LVATTGMYWNNVKEIFSYMIGVGSDG
EEEECCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]