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The map label for this gene is nrdE [H]

Identifier: 118476920

GI number: 118476920

Start: 1364860

End: 1366587

Strand: Direct

Name: nrdE [H]

Synonym: BALH_1212

Alternate gene names: 118476920

Gene position: 1364860-1366587 (Clockwise)

Preceding gene: 118476919

Following gene: 118476921

Centisome position: 25.96

GC content: 36.63

Gene sequence:

>1728_bases
TTGCGACACATTGAACTGAATAATGAAATCACGCAAATGCAGGACGGTTTTTATCAGCTTCATAAAGATAAAGAGGCATT
AGAAGTCTTTATGGAAGAAGCTAGAGAGAATACCGTTCATTTTAATAGCGTGGCAGAGCGAATGGAGTATATGAAAGAAC
ATGATTACTATTACAACGTTCTGGACGAGTATAACTTGGAGGAAGTAGAAGAGGTATATAACATCGCTTATGGTGAAAAC
TTCGAGTTCCAATCTTATATGGCAGCATCTAAGTTTTACAAAGATTATGCGTTAAAAACAAATGATCAAAAACAATACTT
AGAAAGCTATGAAGATCGTGTAGCAATTGTGTCATTATACTTAGGACGCGGTGATGTTGCGAAGGCAAAACAATTCGCAA
GCATGATTGTCAAACAAAACTACCAACCAGCGACACCAACCTTTTTAAATGCGGGAAGAAGCAGAAGAGGAGAAATGGTG
TCTTGTTTCTTGTTAGAGATGGATGATAGCTTAAATTCAATCGGCTTTAACATTAATACTGCAATGCAATTATCAAAAAT
CGGCGGAGGAGTAGCTTTAAATTTATCTAAGCTACGCGCACGTGGTGAGCAAATTAAAGGTATTGACAACGCTGCAAGTG
GTGTAGTACCTGTTATGAAGTTACTTGAAGATTCGTTTTCATATGCGAATCAGCTTGGCCAACGAAAAGGGGCCGGTGCT
GTATACTTAAACATTTTCCATTGGGATATTATTGAATTCCTTGATACAAAGAAAATAAATGCCGATGAGAAGAGCCGTAT
TCAGTCTTTATCAATCGGAATTATCGTTCCAAGTAAGTTCTTTGAGCTTGCTGAGAAAAACGAACCTTTCCATGTTTTCG
CGCCTTATACGGTGTATAAAGAATACGGAAAGCATTTAGATGACATTGATATTGATGAAATGTACGATGAATTAATGAGC
AATCCAAAAGTGAAGAAGAAGCCGCTAGATATTAGTGCGCGTGATATGCTTATTAAAATTGCTATGATTCAGCTTGAGTC
TGGTTATCCGTACTTAATGTTTAAATCAAATGCAAATAACCAACATCCATTGAAGGACATTGGAACTGTGAAGATGTCCA
ACTTGTGTACAGAAATCTTCCAGCTACAAGAAACTTCTGAAATTAACGATTACGGTACAGATGACATTATTCGTCGTGAT
ATTAACTGTAACTTAGGATCGTTAAATATCGTAAATGTAATGGAAAATAAAGAAATTCGTGAAGCAGTTCATGCGGGAAT
GGAAGCTTTAACAGCTGTTTCTGATATGACGATCATTCCGAATGCACCAACTGTGAAAAAAGCAAATGATGAGCTTCATT
CAGTTGGGCTTGGTGCAATGAACTTACACGGATATTTAGCAAAAAATAAAATCGCTTATGAAAGTGCAGAAGCGAAAGAG
TTCGCTCGTACATTCTTTATGATGTTAAATTACTACTCTATTGAGAAAAGTATGGAAATCGCTAAAGAAAAAGGCGAAAC
ATTTAAAGACTTCGATAAATCTGATTATGCGAATGGCGCATACTTTGAAAAGTATGAAACGATTGATTATAGCCCAGTAA
CTGAAAAAGTTCAGCAACTATTTGCCGGAATTCATATTCCTACAAAAGAAGATTGGAATCAATTAAAACGACAAGTAATG
GAGCATGGTTTGTACAATGCGTTAACATGTAGCGCCCCTAGACAGTGA

Upstream 100 bases:

>100_bases
GACAAAATTTCTGCTAAATATGAAGTGCCTATTGTATCAAAATTTGAGTTATCAGGAACAAATAACGATGTAGAATATTT
TAAGGAAAGGGTGCGGGAGA

Downstream 100 bases:

>100_bases
TGTTTAGAGGAAAACGGCTTATATGCAGGAAAGTCCTAAGAGTTCTTTCTACCAAGTACACATAGAAATGTGTGTATGGC
GAGGGGTAATGCCTAAGGTA

Product: ribonucleoside-diphosphate reductase, subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMS
NPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRD
INCNLGSLNIVNVMENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKE
FARTFFMMLNYYSIEKSMEIAKEKGETFKDFDKSDYANGAYFEKYETIDYSPVTEKVQQLFAGIHIPTKEDWNQLKRQVM
EHGLYNALTCSAPRQ

Sequences:

>Translated_575_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMS
NPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRD
INCNLGSLNIVNVMENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKE
FARTFFMMLNYYSIEKSMEIAKEKGETFKDFDKSDYANGAYFEKYETIDYSPVTEKVQQLFAGIHIPTKEDWNQLKRQVM
EHGLYNALTCSAPRQ
>Mature_575_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMS
NPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRD
INCNLGSLNIVNVMENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKE
FARTFFMMLNYYSIEKSMEIAKEKGETFKDFDKSDYANGAYFEKYETIDYSPVTEKVQQLFAGIHIPTKEDWNQLKRQVM
EHGLYNALTCSAPRQ

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=506, Percent_Identity=27.6679841897233, Blast_Score=189, Evalue=6e-48,
Organism=Escherichia coli, GI1789030, Length=555, Percent_Identity=46.1261261261261, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI2367133, Length=437, Percent_Identity=24.0274599542334, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17555242, Length=506, Percent_Identity=27.2727272727273, Blast_Score=186, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6322123, Length=595, Percent_Identity=24.3697478991597, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6320914, Length=509, Percent_Identity=25.5402750491159, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17352481, Length=526, Percent_Identity=27.1863117870722, Blast_Score=185, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 65881; Mature: 65881

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNV
CCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHEEH
LDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLY
HCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEE
LGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINT
ECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCHHHCCCCCHH
AMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
HHHHHHCCCCEEEEHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYK
EEEEEHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHH
EYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANN
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCEEEEECCCCC
QHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVMENKEIR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEHHCCHHHH
EAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKE
HHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHHCHHHHHH
FARTFFMMLNYYSIEKSMEIAKEKGETFKDFDKSDYANGAYFEKYETIDYSPVTEKVQQL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEHHCCCCCCCHHHHHHHHH
FAGIHIPTKEDWNQLKRQVMEHGLYNALTCSAPRQ
HHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNV
CCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHEEH
LDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLY
HCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEE
LGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINT
ECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCHHHCCCCCHH
AMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
HHHHHHCCCCEEEEHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYK
EEEEEHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHH
EYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANN
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCEEEEECCCCC
QHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVMENKEIR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEHHCCHHHH
EAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKE
HHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHHCHHHHHH
FARTFFMMLNYYSIEKSMEIAKEKGETFKDFDKSDYANGAYFEKYETIDYSPVTEKVQQL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEHHCCCCCCCHHHHHHHHH
FAGIHIPTKEDWNQLKRQVMEHGLYNALTCSAPRQ
HHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]