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The map label for this gene is afuA [C]

Identifier: 118476894

GI number: 118476894

Start: 1338817

End: 1339893

Strand: Direct

Name: afuA [C]

Synonym: BALH_1186

Alternate gene names: 118476894

Gene position: 1338817-1339893 (Clockwise)

Preceding gene: 118476892

Following gene: 118476895

Centisome position: 25.47

GC content: 35.28

Gene sequence:

>1077_bases
GTGAAAAAAACAATCTTTAAAGCTGTAGCAACTGGAATGGTATTTTCGTTATTAATGGGGTGTGGTGCAAAGAAAGAAGA
AAGTGCTGGGGCAAAAGTAAAAGATGATAAATTATCTGGATCATTAACTGTTTACACAGCGATTGAAGAAGAACTTGTAC
CAATTTATCTTGATTCTTTTAAAAAGAAATACCCAGATGTGAAGCTGAACATTGTTCGTGATTCAACTGGAGTTATTACA
GCGAAATTACTAGCTGAAGGAAAAAATACACAAGCAGATGTTGTATGGGGAACTGCAGCATCAAGTCTATTAGCTTTAGA
TAAAAAAGATATGTTAAAAGGATACTCTCCAAAAGGAGCGGATCGCGTTCTACCACAATTTAAAGATGATAAACAACCAG
AAAAATGGGTTGGAAATACTGCATTTATGACGGGGATTGCTATAAATAAAGAAGAATTAAAGAAGAAAAATTTACCGATG
CCAGAATCATACGAAGACTTAACGAAACCAGAATATAAAGGAACGCTTGTTATGCCACATCCGGCTTCTTCTGGAACAGG
ATTTTTAACAGTTTCCGCATGGTTACAAATTATGGGTGAAGATAAAGGCTGGGATTACATGAAGAAACTTCATGATAATA
TGGCAACTTATACGCATTCAGGCTCAAAACCAGCGAAATTAGCAGGTGCAGGTGAATATCCAGTTGGCGTATCGATGGTT
TATAGTGCTTTGAAAGAGAAACAAAAAGGTGCACCAGTTGAAGTTGTATTGCCGAAAGAAGGATTAGGTTGGGAAGTAGA
AGCGAACGCACTTATTAAAAAAGATAATGCAAAAAATGAAAAATTAGCACAAGCATTTTTAGATTGGGCAATTACTGATG
ATGTAATGAAGTTATACTTCGAGAAAAATGGATTTGCAACAATTAAAAATGATTATAAACTTCCAGATGGATTCCCTAAA
GATGTGACGGAAAAGTTATACAAAAAGAATGACTTTAAGTGGGCAGCAGAAAATCGCGACAAAATTTTAGAAAAATGGGA
AAAAGAGTTTGGGCAAAAAGCAGAACCGAAAAAGTAA

Upstream 100 bases:

>100_bases
ACAAACAACAAACAAAAGATTAATGTTGGTTTAATACTGCTTTTACACTAGTTGTTTATATTGGAGTTGTACATAAGATT
TGTAAAAGGAGAGAGAGACA

Downstream 100 bases:

>100_bases
GTGAGTGGGAGAGAGAAAAAATGAGCGAATATTTATCAATTCAACACATTCAAAAACAGTTTGATGCATTTACAGCGTTA
AAGGATATTTCTTTTACTGT

Product: ABC transporter solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKKTIFKAVATGMVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIVRDSTGVIT
AKLLAEGKNTQADVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPM
PESYEDLTKPEYKGTLVMPHPASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMV
YSALKEKQKGAPVEVVLPKEGLGWEVEANALIKKDNAKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDYKLPDGFPK
DVTEKLYKKNDFKWAAENRDKILEKWEKEFGQKAEPKK

Sequences:

>Translated_358_residues
MKKTIFKAVATGMVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIVRDSTGVIT
AKLLAEGKNTQADVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPM
PESYEDLTKPEYKGTLVMPHPASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMV
YSALKEKQKGAPVEVVLPKEGLGWEVEANALIKKDNAKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDYKLPDGFPK
DVTEKLYKKNDFKWAAENRDKILEKWEKEFGQKAEPKK
>Mature_358_residues
MKKTIFKAVATGMVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSFKKKYPDVKLNIVRDSTGVIT
AKLLAEGKNTQADVVWGTAASSLLALDKKDMLKGYSPKGADRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPM
PESYEDLTKPEYKGTLVMPHPASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMV
YSALKEKQKGAPVEVVLPKEGLGWEVEANALIKKDNAKNEKLAQAFLDWAITDDVMKLYFEKNGFATIKNDYKLPDGFPK
DVTEKLYKKNDFKWAAENRDKILEKWEKEFGQKAEPKK

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39828; Mature: 39828

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIFKAVATGMVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHH
KKKYPDVKLNIVRDSTGVITAKLLAEGKNTQADVVWGTAASSLLALDKKDMLKGYSPKGA
HHHCCCEEEEEEECCCCEEEEEEHHCCCCCCEEEEECHHHHHHHHCCHHHHHCCCCCCCH
DRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPMPESYEDLTKPEYKGTLVMPH
HHCCCCCCCCCCHHHHCCCHHHEEEEEECHHHHHHCCCCCCCCHHHHCCCCCCCEEEECC
PASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMV
CCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCHHHHH
YSALKEKQKGAPVEVVLPKEGLGWEVEANALIKKDNAKNEKLAQAFLDWAITDDVMKLYF
HHHHHHHHCCCCEEEEECCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EKNGFATIKNDYKLPDGFPKDVTEKLYKKNDFKWAAENRDKILEKWEKEFGQKAEPKK
HCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKTIFKAVATGMVFSLLMGCGAKKEESAGAKVKDDKLSGSLTVYTAIEEELVPIYLDSF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHH
KKKYPDVKLNIVRDSTGVITAKLLAEGKNTQADVVWGTAASSLLALDKKDMLKGYSPKGA
HHHCCCEEEEEEECCCCEEEEEEHHCCCCCCEEEEECHHHHHHHHCCHHHHHCCCCCCCH
DRVLPQFKDDKQPEKWVGNTAFMTGIAINKEELKKKNLPMPESYEDLTKPEYKGTLVMPH
HHCCCCCCCCCCHHHHCCCHHHEEEEEECHHHHHHCCCCCCCCHHHHCCCCCCCEEEECC
PASSGTGFLTVSAWLQIMGEDKGWDYMKKLHDNMATYTHSGSKPAKLAGAGEYPVGVSMV
CCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCEECCCCCCCCHHHHH
YSALKEKQKGAPVEVVLPKEGLGWEVEANALIKKDNAKNEKLAQAFLDWAITDDVMKLYF
HHHHHHHHCCCCEEEEECCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EKNGFATIKNDYKLPDGFPKDVTEKLYKKNDFKWAAENRDKILEKWEKEFGQKAEPKK
HCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]