Click here to switch to the map view.

The map label for this gene is cls

Identifier: 118476770

GI number: 118476770

Start: 1205092

End: 1206636

Strand: Direct

Name: cls

Synonym: BALH_1053

Alternate gene names: 118476770

Gene position: 1205092-1206636 (Clockwise)

Preceding gene: 118476769

Following gene: 118476771

Centisome position: 22.92

GC content: 35.92

Gene sequence:

>1545_bases
ATGAAAAATACGTTAAAGTTGATTTTCTTTGTTTTACTATTGTTCGCATTATTTGTTTCATTACGTATGTTTATTGATGT
GGCATTTTATTCGGATGTAATTGGGATAAAAGATGTTTCAATTTTAGGTATTATTAGTATTTTATTTACGGTATCTGCAT
TTTTAATTGGCTGCGTTATTTTCTTAGAGAACCGTCATCCGTCAAAAACACTTACATGGCTCATTGTGTTAGGTATTTTT
CCGGTATTTGGATTCTTCGCTTATTTATTATTCGGACAAAACTTTCGTAGGAAGAGAATGTTCCAAAAGAAGGCGTTGCT
CGATGAACAAGCGTTTTTACAATATAAGGGGCATGAAGATTACGAAGAACGGATTTTGCGCAATCATAAGCATCAAGAGC
TGTTATTTCGTTTAGCGGATCGCTTAGGCGCTTTAAATATTTCATTTCAAACTGAAACGAGAACATTAACAAATGGAGAT
GAAACGTTTCAGGCCATTTTAGATGGATTAAAACGAGCGAAACATCACATTCATATGGAATATTACATTGTGCGTGATGA
TAAGCTTGGAACAGAAATTAAAGATATTTTAATACAAAAATCAAAAGAGGGCGTAGTCGTTCGTTTTTTATATGATGCGG
TGGGAAGTTTTAAATTATCAAAATCGTATATTGAAGAATTAAACGATGCAGGTGTCGAAATGATTCCGTTCTTCCCTGTG
CGCTTTCCGATTTTAAACGATAAGATTAATTATCGAAACCACCGAAAAATCGTTATTATTGATGGAAATGAAGGGTTTGT
AGGTGGATTAAATATTGGTGATGAATATTTAGGGAAGGATAAGTACTTCGGCTTTTGGCGAGACACACATTTATATTTGC
GTGGTGAAGCTGTACAAAGTTTGCAACTTATTTTCCTTCAAGATTGGTTTTATATGACTGGTGAGGCTGTGCTAGCCCCT
GAATATTTACAAGCGAAAGCAGTTGAGGGAGAGCATTGGGGAGGCGTGCAGCTCGTTGCAGGTGGGCCGGATAATAAATG
GGAAACAATCAAACATTTATATTTTGCTATGATTGCTTCTGCGCGGAAATCCATTTGGATTGCAACGCCGTATTTCATTC
CGGATGATGATATTTTATCCGCATTAAAGGTAGCTGCGCTTGCTGGTATCGATGTTCGTTTGTTAATGCCGAGTAAGCCT
GATAAGCGCACTGTCTTTTACGCATCGAGATCGTATTTCCCAGAGCTTTTAGATGCAGGGGTAAAGATATATGAATACGA
GAAAGGTTTTCTCCATAGTAAAGTTGTCATTGTCGATTCTGATTTAGCTTCAATTGGGACAGCGAATATGGATATGAGAA
GTTTTCATTTGAACTTTGAAGTAAATGCCTTTTTATATGATACAGATAGCATTCGAAAACTTGTTCAAGATTTTAAAGAC
GATTTAGAAGAATCAAGTGAAATTCATGTCGACCGTTTTCATAAAAGGCGTCTTCATAGACGAATTGTTGAATCAACGTA
CCGGTTATTATCACCTTTATTATAA

Upstream 100 bases:

>100_bases
TCGCAATAATTTTCCTGCTAAAACGTAGGCCGATTTCTGTAGTATGAAATCGGCTTTTTATATGAAGAATAGGAAAGCTT
TCTTAGCAAGGAGGTAAGAG

Downstream 100 bases:

>100_bases
AGAGAGATGTCCGAAATTGGGACATCTCTTTTTTAAAAAGGAATTTGTAATAAATTGTAGAATATAAGGAGCTGAAAGGG
TGTGATTCCGATATTTATCG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase 2

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFQAILDGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL

Sequences:

>Translated_514_residues
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFQAILDGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL
>Mature_514_residues
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGD
ETFQAILDGLKRAKHHIHMEYYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=464, Percent_Identity=29.9568965517241, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1787007, Length=334, Percent_Identity=29.6407185628743, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS2_BACAN (Q81TR2)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_843679.1
- RefSeq:   YP_017816.1
- RefSeq:   YP_027384.1
- ProteinModelPortal:   Q81TR2
- IntAct:   Q81TR2
- EnsemblBacteria:   EBBACT00000012500
- EnsemblBacteria:   EBBACT00000015717
- EnsemblBacteria:   EBBACT00000023328
- GeneID:   1087241
- GeneID:   2817072
- GeneID:   2852685
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1204
- KEGG:   bar:GBAA_1204
- KEGG:   bat:BAS1112
- TIGR:   BA_1204
- TIGR:   GBAA_1204
- GeneTree:   EBGT00070000032016
- HOGENOM:   HBG694312
- OMA:   TGMPCLA
- ProtClustDB:   PRK01642
- BioCyc:   BANT260799:BAS1112-MONOMER
- BioCyc:   BANT261594:GBAA1204-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.-

Molecular weight: Translated: 59502; Mature: 59502

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 254-254 ACT_SITE 256-256 ACT_SITE 261-261 ACT_SITE 432-432 ACT_SITE 434-434 ACT_SITE 439-439

Signals:

None

Transmembrane regions:

HASH(0x17d51a6c)-; HASH(0x1960f2d0)-; HASH(0x199bbc80)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
YEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGDETFQAILDGLKRAKHHIHME
HHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
EEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEECCEE
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCCEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MKNTLKLIFFVLLLFALFVSLRMFIDVAFYSDVIGIKDVSILGIISILFTVSAFLIGCVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVFGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
YEERILRNHKHQELLFRLADRLGALNISFQTETRTLTNGDETFQAILDGLKRAKHHIHME
HHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTEIKDILIQKSKEGVVVRFLYDAVGSFKLSKSYIEELNDAGVEMIPFFPV
EEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEECCEE
RFPILNDKINYRNHRKIVIIDGNEGFVGGLNIGDEYLGKDKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCCEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAKAVEGEHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTDSIRKLVQDFKD
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLEESSEIHVDRFHKRRLHRRIVESTYRLLSPLL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629