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The map label for this gene is lplA [H]

Identifier: 118476690

GI number: 118476690

Start: 1115886

End: 1116875

Strand: Direct

Name: lplA [H]

Synonym: BALH_0968

Alternate gene names: 118476690

Gene position: 1115886-1116875 (Clockwise)

Preceding gene: 118476689

Following gene: 118476691

Centisome position: 21.23

GC content: 35.96

Gene sequence:

>990_bases
ATGTTATTTATTGATAATAAAGGGATTACAGATCCTAGAATAAACTTAGCGATTGAAGAATATTGTGTGAAGAATTTAGA
TATTAACGAAACATATTTACTGTTCTACATTAATGAGCCTTCGATTATTATTGGTAAAAACCAAAACACAGTAGAAGAAA
TTAATGCTGATTACGTAAAAGAAAAAGGGATTCATGTCGTTCGTCGTCTATCAGGCGGGGGCGCAGTATATCACGATTTA
GGAAACTTAAACTTCAGCTTTATTACGAAAGATGATGGAGACAGTTTCAGCAACTTCAAAAAATTTACAGAGCCTGTAAC
GAAAGCGCTTGGTAAATTAGGCGTAAATGCAGAACTAAGTGGCCGTAACGACATTTTAGCTGAAGGTAGAAAAATTTCAG
GTAACGCGCAGTTCTCAACGAAAGGTCGTATGTTCAGTCATGGTACGTTATTATTTGACTCTGAAATCGATCACGTCGTA
TCAGCGTTAAAAGTAAAAATGGATAAGATTCAATCAAAAGGTATTAAATCAATCCGTAGCCGCGTTGCGAACATTACAGA
GTTCTTAAGCGAAAAAATGACGACTGAAGAGTTTAGACAACTTCTTCTAGAAACAATTTTCGAAGGGGAAACAGAAATCC
CGACGTACGAATTAACAGAAAATGATTGGAAAGAAATCCATAAACTATCTGAAGAACGTTACCAAAATTGGGACTGGAAC
TACGGGAAATCTCCGAAGTTCAACTTACAACATTCACACCGCTTCCCAGTTGGACAAGTTGACGTTCGTCTTGAAGTGAA
AAAAGGAACTGTAACAGAATGTAAAATTTACGGTGACTTCTTCGGTTCATTAGATGTGCATGACATTGAAGAGCGTCTAA
CAGGTGTACAATTTGATAAAGATGCATTCACTGCAGCTTTAGAAGACGTAGATATTAAACGCTACTTTGGTAATATTACA
ACAGAAGATTTCTTACATTTATTCTTCTAA

Upstream 100 bases:

>100_bases
AGGCAATCCAGCTGATTTAGTAACAGAAGCAAAACAAATTTTCAGTGGCCATATTACGTTAGCGCATAGTGGCTACGTAT
GGAACTCATGAGGTGATACG

Downstream 100 bases:

>100_bases
AAATAAGGGAGGCTGCCATAACGGGCAGCCTCTTTCTTTTTGCGTAGATTGTCATGTTTTGTCGGTAAGTTGATATATTT
AAAAAATCGCTGATATATTC

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLSEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTAALEDVDIKRYFGNIT
TEDFLHLFF

Sequences:

>Translated_329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLSEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTAALEDVDIKRYFGNIT
TEDFLHLFF
>Mature_329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLSEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTAALEDVDIKRYFGNIT
TEDFLHLFF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729884, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729882, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729880, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729878, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1790846, Length=305, Percent_Identity=34.4262295081967, Blast_Score=177, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI71981909, Length=244, Percent_Identity=35.2459016393443, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322415, Length=283, Percent_Identity=30.7420494699647, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28573464, Length=283, Percent_Identity=31.4487632508834, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37710; Mature: 37710

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLSEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCH
DAFTAALEDVDIKRYFGNITTEDFLHLFF
HHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLSEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCH
DAFTAALEDVDIKRYFGNITTEDFLHLFF
HHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]