Click here to switch to the map view.

The map label for this gene is alsS [H]

Identifier: 118476516

GI number: 118476516

Start: 895097

End: 896785

Strand: Direct

Name: alsS [H]

Synonym: BALH_0784

Alternate gene names: 118476516

Gene position: 895097-896785 (Clockwise)

Preceding gene: 118476513

Following gene: 118476517

Centisome position: 17.03

GC content: 40.67

Gene sequence:

>1689_bases
TTGAGTACAGGTGTAAAAGCAAACGACGTGAAGACAAAAACAAAAGGAGCAGATCTTGTTGTTGATTGTTTAATTAAACA
AGGTGTTACACATGTTTTCGGGATTCCAGGAGCGAAGATTGATTCTGTATTTGATGTACTGCAAGAAAGAGGACCAGAGT
TAATTGTTTGTCGTCATGAACAAAACGCAGCATTTATGGCAGCTGCTATTGGTAGATTAACAGGGAAACCGGGCGTATGT
CTTGTAACTTCAGGACCAGGGACATCAAATTTAGCGACAGGTCTTGTTACTGCGAATGCGGAGAGTGATCCCGTTGTTGC
TTTAGCTGGTGCAGTTCCACGTACGGATCGATTAAAACGTACGCATCAATCTATGGATAATGCAGCACTATTCGAACCAA
TCACAAAATATAGCGTAGAAGTAGAGCATCCTGATAATGTGCCAGAAGCATTATCAAATGCATTCCGAAGTGCGACTTCC
ACAAATCCAGGAGCTACTCTAGTAAGTCTGCCGCAAGACGTTATGACTGCGGAAACGACTGTAGAGTCTATCGGTGCGCT
TTCTAAGCCACAGCTTGGAATCGCTCCCACACATGATATTACATATGTAGTAGAAAAAATAAAAGCAGCAAAATTACCGG
TTATTTTACTCGGTATGAGAGCGAGCACAAATGAAGTGACGAAAGCTGTTCGTGAATTAATTGCGGATACAGAACTACCT
GTCGTTGAAACATATCAAGCAGCTGGTGCGATTTCACGTGAATTAGAAGATCATTTCTTCGGCCGTGTTGGATTATTCCG
TAACCAACCAGGTGATATTTTACTAGAAGAAGCAGACCTTGTTATTTCTATGGGTTATGACCCAATTGAGTATGATCCAA
AGTTCTGGAATAAACTTGGGGACAGAACGATTATTCATCTTGATGACCATCAAGCAGATATTGATCATGATTACCAACCA
GAGCGTGAATTAATTGGTGATATTGCCTTAACAGTAAATAGCATCGCAGAAAAATTACCGAAACTTGTGTTAAGTACGAA
ATCAGAAGCAGTACTAGAACGATTACGTGCGAAATTATCAGAACAAGCAGAAGTTCCAAATCGTCCTTCAGAAGGTGTTA
CGCATCCACTTCAAGTGATTCGTACACTTCGTTCTTTAATTAGTGACGATACAACCGTTACATGTGACATCGGTTCACAT
TCTATTTGGATGGCAAGATGTTTCCGTTCTTATGAACCACGTAGATTATTATTTAGTAACGGTATGCAAACGTTAGGTGT
TGCACTTCCTTGGGCAATCGCAGCTACTTTAGTAGAACCAGGTAAAAAAGTAGTTTCCGTATCAGGTGACGGTGGGTTCT
TATTCTCAGCGATGGAGTTAGAAACGGCGGTACGCTTAAATTCTCCAATCGTCCATCTCGTTTGGAGAGACGGCACATAT
GATATGGTTGCGTTCCAACAAATGATGAAATATGGTAGAACATCAGCTACAGAGTTCGGTGATGTTGATATTGTGAAATA
TGCAGAAAGTTTCGGTGCGACAGGTCTTCGCGTTAACACGCCTGATGAATTAGAAGGTGTATTAAAATCCGCACTAGCAG
CAGACGGCCCTGTCATTATTGATATTCCAATCGATTACCGTGACAATATTAAATTAAGCGAAAAATTATTACCAAACCAA
TTAAACTAA

Upstream 100 bases:

>100_bases
ATTTGTACTATAATGATAAATAATACTTCACTACATTAATTAACTAGGTGAAGTTTAAAGTCTTTATTACACACATTATG
AAATAAAGGAATGATTAGTT

Downstream 100 bases:

>100_bases
TGGAGGCAGATTTGAGATGACTGTTGCGCAATTAATTGATATTGATGCAAAAAGAACGAAAACGAATAACGAAGTATATC
AAACATCTACAATGCTTGCG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVC
LVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATS
TNPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADTELP
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISMGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQP
ERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSH
SIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
DMVAFQQMMKYGRTSATEFGDVDIVKYAESFGATGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLLPNQ
LN

Sequences:

>Translated_562_residues
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVC
LVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATS
TNPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADTELP
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISMGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQP
ERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSH
SIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
DMVAFQQMMKYGRTSATEFGDVDIVKYAESFGATGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLLPNQ
LN
>Mature_561_residues
STGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVCL
VTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATST
NPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADTELPV
VETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISMGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQPE
RELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHS
IWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTYD
MVAFQQMMKYGRTSATEFGDVDIVKYAESFGATGLRVNTPDELEGVLKSALAADGPVIIDIPIDYRDNIKLSEKLLPNQL
N

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=26.7256637168142, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI21361361, Length=601, Percent_Identity=25.4575707154742, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=29.4014084507042, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=29.5081967213115, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1786717, Length=573, Percent_Identity=26.7015706806283, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1787096, Length=536, Percent_Identity=26.6791044776119, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=27.2232304900181, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=575, Percent_Identity=26.2608695652174, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=556, Percent_Identity=27.3381294964029, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=556, Percent_Identity=27.3381294964029, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=27.9359430604982, Blast_Score=202, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=477, Percent_Identity=28.5115303983228, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6323163, Length=548, Percent_Identity=23.1751824817518, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922626, Length=557, Percent_Identity=26.5709156193896, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61000; Mature: 60869

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHE
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEEECC
QNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHEEEEEECCCCCCEEEEECCCCCHHHHHH
THQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTAETT
HHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
VESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADTELP
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISMGYDPIEYDPKFWNKLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHCC
DRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLLFSN
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHC
GMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCH
DMVAFQQMMKYGRTSATEFGDVDIVKYAESFGATGLRVNTPDELEGVLKSALAADGPVII
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEE
DIPIDYRDNIKLSEKLLPNQLN
EEECCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
STGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEEECC
QNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHEEEEEECCCCCCEEEEECCCCCHHHHHH
THQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTAETT
HHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
VESIGALSKPQLGIAPTHDITYVVEKIKAAKLPVILLGMRASTNEVTKAVRELIADTELP
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISMGYDPIEYDPKFWNKLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHCC
DRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLLFSN
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHC
GMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCH
DMVAFQQMMKYGRTSATEFGDVDIVKYAESFGATGLRVNTPDELEGVLKSALAADGPVII
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEE
DIPIDYRDNIKLSEKLLPNQLN
EEECCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]