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The map label for this gene is blT [H]

Identifier: 118476493

GI number: 118476493

Start: 867778

End: 868989

Strand: Direct

Name: blT [H]

Synonym: BALH_0761

Alternate gene names: 118476493

Gene position: 867778-868989 (Clockwise)

Preceding gene: 118476492

Following gene: 118476495

Centisome position: 16.51

GC content: 36.88

Gene sequence:

>1212_bases
GTGTTAAAAGTGAAGAAACCGATAAAAGAACAAAAGATGGTATTAGTCATTCTCTTAAGTAATATATTTATCGCTTTTTT
AGGGATTGGATTAATTATTCCGGTTATGCCGTCCTTTATGAACGAGATGCATTTAACAGGGAAGACGATGGGGTATCTCG
TTGCAGTATTTGCGATGGCTCAGCTTATTGCTTCACCTATTACAGGCCGGTGGGTCGACCTTTACGGTAGGAAGAAAATG
ATAATCATTGGATTATTTATTTTTGGTGTTTCAGAGCTTCTTTTCGGATTAGGAACAGATGTATGGATGCTTTATGTAGC
ACGGGTGTTAGGCGGAATTAGTGCTGCGTTTATTATGCCTGGTGTTACGGCATATGTAGCGGATATTACATCTATCCAAG
AACGTCCAAAAGCGATGGGATATTTATCTGCGGCGATTAGTACTGGGTTTATTATAGGGCCTGGAATTGGCGGATTTATT
GCAGAGTACGGTATACGTGTGCCGTTTTTCGTTGCAGCAGCAATTGCTTTTATAGCATGTGTCATTTCGATCTTTATTTT
AAAAGAACCATTAACGAAAGAAGAGCTTGCAGAGATTTCTTCTAATACGAAAGAATCAAGCTTTATTGGTGATTTAAAGA
AATCATTACATCCAATGTATGCAATTGCATTTATTATCGTATTTGTACTTGCATTCGGTTTATCGGCGTATGAAACTGTG
TTTAGTCTGTTTTCTGATCATAAGTTTGGATTCACACCGAAAGATATTGCTGCAATTATTACAATTAGTTCAATCTTTGG
GGTAGTTGTACAAGTATTTATGTTTGGAAAATTAGTTGATATGTTCGGTGAAAAAGTGTTAATTCAAATCTGTTTAATTG
TAGGTGCAGTATTAGCATTCGTTTCAACTGTCGTCTTTAATTATTGGATTGTACTTCTCGTTACGTGCTTTATTTTCCTT
GCATTTGATTTACTTCGTCCAGCTTTAACGACGTTTTTATCGAAAGCAGCTGGAAAAGAGCAAGGATTCGTTGCTGGTAT
GAACTCGACTTATACGAGTTTAGGAAATATAGCAGGACCAGCGATGGGCGGAATACTATTTGATATGAATATTCATTATC
CATATGCATTTTCAGGAGTTGTCTTAATAGTTGGTCTCGCTATTACATTTATGTGGAGAGAGAAACAGCTAGCTGAGAGT
TTTGCGAAGTAA

Upstream 100 bases:

>100_bases
CTAATGAGTGGGACATCTTCACTCATTATTAGCCCATAAATAGCAGGATAAAGAAAAAGCTTCTTGGGCATTATTTTTTG
AGAGGAATTTACGGGAAGAG

Downstream 100 bases:

>100_bases
TGAGAGTGAAGAATGATTAAAAAGCCCCTTTTCGCAAGGGGCTTTTTTGTATGTTAATGGGGGAGGGGCTGTATCCACAA
CTTCGCAAAATTTGCCCAAC

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MLKVKKPIKEQKMVLVILLSNIFIAFLGIGLIIPVMPSFMNEMHLTGKTMGYLVAVFAMAQLIASPITGRWVDLYGRKKM
IIIGLFIFGVSELLFGLGTDVWMLYVARVLGGISAAFIMPGVTAYVADITSIQERPKAMGYLSAAISTGFIIGPGIGGFI
AEYGIRVPFFVAAAIAFIACVISIFILKEPLTKEELAEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETV
FSLFSDHKFGFTPKDIAAIITISSIFGVVVQVFMFGKLVDMFGEKVLIQICLIVGAVLAFVSTVVFNYWIVLLVTCFIFL
AFDLLRPALTTFLSKAAGKEQGFVAGMNSTYTSLGNIAGPAMGGILFDMNIHYPYAFSGVVLIVGLAITFMWREKQLAES
FAK

Sequences:

>Translated_403_residues
MLKVKKPIKEQKMVLVILLSNIFIAFLGIGLIIPVMPSFMNEMHLTGKTMGYLVAVFAMAQLIASPITGRWVDLYGRKKM
IIIGLFIFGVSELLFGLGTDVWMLYVARVLGGISAAFIMPGVTAYVADITSIQERPKAMGYLSAAISTGFIIGPGIGGFI
AEYGIRVPFFVAAAIAFIACVISIFILKEPLTKEELAEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETV
FSLFSDHKFGFTPKDIAAIITISSIFGVVVQVFMFGKLVDMFGEKVLIQICLIVGAVLAFVSTVVFNYWIVLLVTCFIFL
AFDLLRPALTTFLSKAAGKEQGFVAGMNSTYTSLGNIAGPAMGGILFDMNIHYPYAFSGVVLIVGLAITFMWREKQLAES
FAK
>Mature_403_residues
MLKVKKPIKEQKMVLVILLSNIFIAFLGIGLIIPVMPSFMNEMHLTGKTMGYLVAVFAMAQLIASPITGRWVDLYGRKKM
IIIGLFIFGVSELLFGLGTDVWMLYVARVLGGISAAFIMPGVTAYVADITSIQERPKAMGYLSAAISTGFIIGPGIGGFI
AEYGIRVPFFVAAAIAFIACVISIFILKEPLTKEELAEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETV
FSLFSDHKFGFTPKDIAAIITISSIFGVVVQVFMFGKLVDMFGEKVLIQICLIVGAVLAFVSTVVFNYWIVLLVTCFIFL
AFDLLRPALTTFLSKAAGKEQGFVAGMNSTYTSLGNIAGPAMGGILFDMNIHYPYAFSGVVLIVGLAITFMWREKQLAES
FAK

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI217416366, Length=422, Percent_Identity=21.3270142180095, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI39753965, Length=326, Percent_Identity=25.1533742331288, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI22749525, Length=161, Percent_Identity=27.3291925465839, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI166064062, Length=383, Percent_Identity=23.7597911227154, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI166064060, Length=383, Percent_Identity=23.7597911227154, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI118582257, Length=141, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787291, Length=404, Percent_Identity=24.7524752475248, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081738, Length=348, Percent_Identity=22.9885057471264, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71996331, Length=382, Percent_Identity=24.6073298429319, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17551570, Length=163, Percent_Identity=32.5153374233129, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17738085, Length=354, Percent_Identity=25.7062146892655, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21357569, Length=371, Percent_Identity=25.8760107816712, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24656463, Length=371, Percent_Identity=25.8760107816712, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24656468, Length=371, Percent_Identity=25.8760107816712, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI161080926, Length=371, Percent_Identity=25.8760107816712, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161080924, Length=371, Percent_Identity=25.8760107816712, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137160, Length=225, Percent_Identity=26.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221513297, Length=332, Percent_Identity=22.5903614457831, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43891; Mature: 43891

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]