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The map label for this gene is dacA [H]

Identifier: 118475787

GI number: 118475787

Start: 17026

End: 18360

Strand: Direct

Name: dacA [H]

Synonym: BALH_0010

Alternate gene names: 118475787

Gene position: 17026-18360 (Clockwise)

Preceding gene: 118475786

Following gene: 118475788

Centisome position: 0.32

GC content: 36.03

Gene sequence:

>1335_bases
TTGGGGGTAGCAAAAGTGAAAGGTATGTTTTGCAAAAGATTCATTGCACTAGTAACGATGCTTACACTAGTTTGTAGTAT
GTTGTTACCATATAGCAATGCGTCAGCAGAAACAGGATCTGCTTTAAACATTGAAGCAGGTGCGGCAATTTTAGTTGAAG
CGAATTCTGGAAAAATTTTATATCAAAAGAATGCAGATGAATTATTATCAATTGCTAGTATGACAAAGATGATGAGTGAA
TATTTAGTGAACGAGGCTGTTGCGAAAGGGAAACTTAAGTGGGATCAAAAAGTTAAGGTTTCTGAATATGCGTATAAAAT
TTCGCAAGATCGTTCATTATCGAATGTGCCGCTACGAAATGGTGAGTCTTATACAGTAAAAGAGCTATATGAAGCAATGG
CAATTTATTCTGCTAATGGTGCAACGATAGCTTTAGCTGAAGCAATTGGTGGAAAAGAAGTAGACTTTGTAAAACTGATG
AATAATAAGTCGAAAGAGCTTGGATTAAAAAATTACAAGTTCGTAAATTCTACCGGCTTAACAAACAAAGATTTAAAAGG
ACAGCACCCTGAGGGGACAAATCCAGATGAAGAAAATAAAATGGCTGCAAGAGATGTGGCGATTTTAGCACAACGTCTTA
TTCAGGACTTCCCTAAAACATTAGATACAGCAAAAATTTCAAAAAAGACATTCCGTGAAGGTACTACAGATCGAATTGAC
ATGATTAACTGGAACTGGATGTTACCAGGTTTATCTAAGGCGTACGATGGTGTAGATGGCTTGAAAACAGGATCTACTCC
AGAAGCGGGAGAATGTTTCACTGGTACAATTGAAAAAGATGGTATGCGTTTTATTTCTGTAGTTATTAACACGAAGTCTC
GTGATGCACGTTTTGATGAGACAAAGAAGTTATATGATTATGGATTTGCTAACTTTGAAATGAAACAAATGTATAAAAAA
GGTTCTTCAGTAAAAGGACAAGAAACAGTGCGAGTAGAAAATGCGAAAGATAAAGATGTAGCAGTTCAAACAAAGCAAGC
TGTTTCACTTCCAGTAGCGAAAGGAAGTAAAGATGTTTATAAAACAGAATTAAAAGAAGCAAATAAAGGACAAGAAGCAC
CTATTAAAAAAGGTGCTGTACTTGGTCAAATGGTAATCACACCAAAAGACACGAGTGATCCTGGATTTTTATCTGGTAAA
TCATTGCAAATAGACCTTGTAACAAAATCTGCAGTAGAAGAGGCGAATTGGTTTACTCGTTCTATGCGCGGAATTGGTTC
TTTCTTTAGTGGTATATGGAATAGTGCTGTTGATACAGTAAAGGGTTGGTTTTAA

Upstream 100 bases:

>100_bases
ACTCATTATAATGTCTTATATACAAATAGACGGAGATTTGATATCTCTGTCTATTTTTTTTTGATTATGTTAGAATAACG
GTTATGTGAGTACATAGATA

Downstream 100 bases:

>100_bases
TAGCTCCTCGTGGTAGGGGCTTTTTTTTATTCCTATTTTTCATACCGACTTTATGAAAAAGTAGTGGACAAGCATCTGAT
AGTTAGTGGTAGAATGTAAG

Product: D-alanyl-D-alanine carboxypeptidase A

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MGVAKVKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSE
YLVNEAVAKGKLKWDQKVKVSEYAYKISQDRSLSNVPLRNGESYTVKELYEAMAIYSANGATIALAEAIGGKEVDFVKLM
NNKSKELGLKNYKFVNSTGLTNKDLKGQHPEGTNPDEENKMAARDVAILAQRLIQDFPKTLDTAKISKKTFREGTTDRID
MINWNWMLPGLSKAYDGVDGLKTGSTPEAGECFTGTIEKDGMRFISVVINTKSRDARFDETKKLYDYGFANFEMKQMYKK
GSSVKGQETVRVENAKDKDVAVQTKQAVSLPVAKGSKDVYKTELKEANKGQEAPIKKGAVLGQMVITPKDTSDPGFLSGK
SLQIDLVTKSAVEEANWFTRSMRGIGSFFSGIWNSAVDTVKGWF

Sequences:

>Translated_444_residues
MGVAKVKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSE
YLVNEAVAKGKLKWDQKVKVSEYAYKISQDRSLSNVPLRNGESYTVKELYEAMAIYSANGATIALAEAIGGKEVDFVKLM
NNKSKELGLKNYKFVNSTGLTNKDLKGQHPEGTNPDEENKMAARDVAILAQRLIQDFPKTLDTAKISKKTFREGTTDRID
MINWNWMLPGLSKAYDGVDGLKTGSTPEAGECFTGTIEKDGMRFISVVINTKSRDARFDETKKLYDYGFANFEMKQMYKK
GSSVKGQETVRVENAKDKDVAVQTKQAVSLPVAKGSKDVYKTELKEANKGQEAPIKKGAVLGQMVITPKDTSDPGFLSGK
SLQIDLVTKSAVEEANWFTRSMRGIGSFFSGIWNSAVDTVKGWF
>Mature_443_residues
GVAKVKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSEY
LVNEAVAKGKLKWDQKVKVSEYAYKISQDRSLSNVPLRNGESYTVKELYEAMAIYSANGATIALAEAIGGKEVDFVKLMN
NKSKELGLKNYKFVNSTGLTNKDLKGQHPEGTNPDEENKMAARDVAILAQRLIQDFPKTLDTAKISKKTFREGTTDRIDM
INWNWMLPGLSKAYDGVDGLKTGSTPEAGECFTGTIEKDGMRFISVVINTKSRDARFDETKKLYDYGFANFEMKQMYKKG
SSVKGQETVRVENAKDKDVAVQTKQAVSLPVAKGSKDVYKTELKEANKGQEAPIKKGAVLGQMVITPKDTSDPGFLSGKS
LQIDLVTKSAVEEANWFTRSMRGIGSFFSGIWNSAVDTVKGWF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=421, Percent_Identity=30.8788598574822, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786851, Length=435, Percent_Identity=28.2758620689655, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082022, Length=398, Percent_Identity=26.8844221105528, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI87082054, Length=241, Percent_Identity=29.045643153527, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48810; Mature: 48678

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVAKVKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCEEE
YQKNADELLSIASMTKMMSEYLVNEAVAKGKLKWDQKVKVSEYAYKISQDRSLSNVPLRN
EECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
GESYTVKELYEAMAIYSANGATIALAEAIGGKEVDFVKLMNNKSKELGLKNYKFVNSTGL
CCCEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHCCCCCEEEECCCCC
TNKDLKGQHPEGTNPDEENKMAARDVAILAQRLIQDFPKTLDTAKISKKTFREGTTDRID
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
MINWNWMLPGLSKAYDGVDGLKTGSTPEAGECFTGTIEKDGMRFISVVINTKSRDARFDE
EEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCCHHH
TKKLYDYGFANFEMKQMYKKGSSVKGQETVRVENAKDKDVAVQTKQAVSLPVAKGSKDVY
HHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEHHHHHCCCCCCCCHHHH
KTELKEANKGQEAPIKKGAVLGQMVITPKDTSDPGFLSGKSLQIDLVTKSAVEEANWFTR
HHHHHHCCCCCCCCCHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHH
SMRGIGSFFSGIWNSAVDTVKGWF
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GVAKVKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCEEE
YQKNADELLSIASMTKMMSEYLVNEAVAKGKLKWDQKVKVSEYAYKISQDRSLSNVPLRN
EECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
GESYTVKELYEAMAIYSANGATIALAEAIGGKEVDFVKLMNNKSKELGLKNYKFVNSTGL
CCCEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHCCCCCEEEECCCCC
TNKDLKGQHPEGTNPDEENKMAARDVAILAQRLIQDFPKTLDTAKISKKTFREGTTDRID
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
MINWNWMLPGLSKAYDGVDGLKTGSTPEAGECFTGTIEKDGMRFISVVINTKSRDARFDE
EEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCCHHH
TKKLYDYGFANFEMKQMYKKGSSVKGQETVRVENAKDKDVAVQTKQAVSLPVAKGSKDVY
HHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEHHHHHCCCCCCCCHHHH
KTELKEANKGQEAPIKKGAVLGQMVITPKDTSDPGFLSGKSLQIDLVTKSAVEEANWFTR
HHHHHHCCCCCCCCCHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHH
SMRGIGSFFSGIWNSAVDTVKGWF
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]