Click here to switch to the map view.

The map label for this gene is sepA [H]

Identifier: 117922007

GI number: 117922007

Start: 4283246

End: 4284328

Strand: Direct

Name: sepA [H]

Synonym: Shewana3_3571

Alternate gene names: 117922007

Gene position: 4283246-4284328 (Clockwise)

Preceding gene: 117922005

Following gene: 117922008

Centisome position: 86.14

GC content: 51.52

Gene sequence:

>1083_bases
ATGAAATCATATCGACTGCTCCCCTTGGCGGCTTTGTTGCCTGCCCTACTGACGGCCTGTACACCCGCGGAAAGCTCAGT
ACAGGCGAGTGCTATTCGTCCGGTAAAACTCTTTGAGGTGATGCAACTCGAAGGGGGAGATTTTAGGACATTTCCGGCCC
GCGTCAGTGCCAATAGCCGCGCCGAGTTATCCTTTAGGATTTCAGGCGAGCTGACAGATCTCGCATTAGTGGAAGGGCAA
CAGATCCGCCAAGGCAGCCTCTTGGCTAAGCTCGACGACAGGGATGCCTATAACAATTTAATGACCCGCGAAGCCGAGCA
TGAACTCTTGGCGGCGGATTTTCAGCGTAAAACCGAACTCCTCAAGCGCAAGTTAATCTCCCAAGCCGAGTTTGATAGCG
CCCAAGCGCAGCTTAAATCGGCCAAAGCGGCCTTGGCCGCCGCCCGTGATCAGTTAAGTTATACCAAGCTTATTGCTCCC
TTTAGTGGCACTGTGGCCAAACGTTTAGTGGATAACCACCAAATCGTACAGGCCAACCAAGGGATTTTAACGCTGCAAAA
CAACAGCTTGCTCGATGTTAGCATTCAAGTTCCCGAGGCGATGGCGGCAAGCTTAAACAACTATATTCAGCAGCAAAACT
TTACCGCTAAGGTGCGTTTTAGCGCATTAGCAGGGATGGAGTTTGATGCGAAATTCAAAGAATACTCGACCCAAGTGACC
CCGGGTACTCAGGCCTATGAAGTGGTGTTTTCCTTACCTCAACCTAAAGATATTCAATTACTTCCGGGCATGAGCGCCGA
GTTAACCCTCGCCTTAGTGAAAACCCCAGATCAAACCGCATCGGCCATAGTGCCAGTCACCGCCATCGATAAACGCGATC
AGGATGGCCAAGTTCAAGTCTGGGTCTATCAAGCGCAATCGGGCGAAGTTAAACCCACGGCAGTGACCTTAGGCCGAGTC
ACCACCGAAGGCATTGAAGTCTTAGGCGGCATCAACAAAGGTGACTTGATTGTCAGCGCGGGGGTATCCCAATTATCCGA
CGGAATGAAAGTGAAGCCACTACGCTGGCAACGCGGAGTGTAA

Upstream 100 bases:

>100_bases
GGATTCATAATCCAAAAGTATCCCGTTTATTGCAACAAATTTAGCTGTTATCTTCGCGCCCCTGAAATGCTGATAAATTG
TTATCGAGGACTCCCCTACC

Downstream 100 bases:

>100_bases
TTCATGAACATTGCTGAGTATTCCATCCGCCACAAAGTGATTAGCTGGATGTTTGTGCTACTGCTGCTCGTGGGCGGTGG
TGTGAGTTTTACCGGTCTTG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKSYRLLPLAALLPALLTACTPAESSVQASAIRPVKLFEVMQLEGGDFRTFPARVSANSRAELSFRISGELTDLALVEGQ
QIRQGSLLAKLDDRDAYNNLMTREAEHELLAADFQRKTELLKRKLISQAEFDSAQAQLKSAKAALAAARDQLSYTKLIAP
FSGTVAKRLVDNHQIVQANQGILTLQNNSLLDVSIQVPEAMAASLNNYIQQQNFTAKVRFSALAGMEFDAKFKEYSTQVT
PGTQAYEVVFSLPQPKDIQLLPGMSAELTLALVKTPDQTASAIVPVTAIDKRDQDGQVQVWVYQAQSGEVKPTAVTLGRV
TTEGIEVLGGINKGDLIVSAGVSQLSDGMKVKPLRWQRGV

Sequences:

>Translated_360_residues
MKSYRLLPLAALLPALLTACTPAESSVQASAIRPVKLFEVMQLEGGDFRTFPARVSANSRAELSFRISGELTDLALVEGQ
QIRQGSLLAKLDDRDAYNNLMTREAEHELLAADFQRKTELLKRKLISQAEFDSAQAQLKSAKAALAAARDQLSYTKLIAP
FSGTVAKRLVDNHQIVQANQGILTLQNNSLLDVSIQVPEAMAASLNNYIQQQNFTAKVRFSALAGMEFDAKFKEYSTQVT
PGTQAYEVVFSLPQPKDIQLLPGMSAELTLALVKTPDQTASAIVPVTAIDKRDQDGQVQVWVYQAQSGEVKPTAVTLGRV
TTEGIEVLGGINKGDLIVSAGVSQLSDGMKVKPLRWQRGV
>Mature_360_residues
MKSYRLLPLAALLPALLTACTPAESSVQASAIRPVKLFEVMQLEGGDFRTFPARVSANSRAELSFRISGELTDLALVEGQ
QIRQGSLLAKLDDRDAYNNLMTREAEHELLAADFQRKTELLKRKLISQAEFDSAQAQLKSAKAALAAARDQLSYTKLIAP
FSGTVAKRLVDNHQIVQANQGILTLQNNSLLDVSIQVPEAMAASLNNYIQQQNFTAKVRFSALAGMEFDAKFKEYSTQVT
PGTQAYEVVFSLPQPKDIQLLPGMSAELTLALVKTPDQTASAIVPVTAIDKRDQDGQVQVWVYQAQSGEVKPTAVTLGRV
TTEGIEVLGGINKGDLIVSAGVSQLSDGMKVKPLRWQRGV

Specific function: Probable periplasmic linker protein component of the sepABC efflux pump with unknown specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=322, Percent_Identity=24.8447204968944, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081796, Length=347, Percent_Identity=23.9193083573487, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786668, Length=327, Percent_Identity=26.2996941896024, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39158; Mature: 39158

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYRLLPLAALLPALLTACTPAESSVQASAIRPVKLFEVMQLEGGDFRTFPARVSANSR
CCCCEECHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHEEECCCCCEEECCCEECCCCC
AELSFRISGELTDLALVEGQQIRQGSLLAKLDDRDAYNNLMTREAEHELLAADFQRKTEL
EEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKRKLISQAEFDSAQAQLKSAKAALAAARDQLSYTKLIAPFSGTVAKRLVDNHQIVQANQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCEEEEECC
GILTLQNNSLLDVSIQVPEAMAASLNNYIQQQNFTAKVRFSALAGMEFDAKFKEYSTQVT
CEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHCCCCC
PGTQAYEVVFSLPQPKDIQLLPGMSAELTLALVKTPDQTASAIVPVTAIDKRDQDGQVQV
CCCCEEEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCCEEE
WVYQAQSGEVKPTAVTLGRVTTEGIEVLGGINKGDLIVSAGVSQLSDGMKVKPLRWQRGV
EEEECCCCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEECCHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure
MKSYRLLPLAALLPALLTACTPAESSVQASAIRPVKLFEVMQLEGGDFRTFPARVSANSR
CCCCEECHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHEEECCCCCEEECCCEECCCCC
AELSFRISGELTDLALVEGQQIRQGSLLAKLDDRDAYNNLMTREAEHELLAADFQRKTEL
EEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKRKLISQAEFDSAQAQLKSAKAALAAARDQLSYTKLIAPFSGTVAKRLVDNHQIVQANQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCEEEEECC
GILTLQNNSLLDVSIQVPEAMAASLNNYIQQQNFTAKVRFSALAGMEFDAKFKEYSTQVT
CEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHCCCCC
PGTQAYEVVFSLPQPKDIQLLPGMSAELTLALVKTPDQTASAIVPVTAIDKRDQDGQVQV
CCCCEEEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCCEEE
WVYQAQSGEVKPTAVTLGRVTTEGIEVLGGINKGDLIVSAGVSQLSDGMKVKPLRWQRGV
EEEECCCCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEECCHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9037074 [H]