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The map label for this gene is dmlR [H]

Identifier: 117921949

GI number: 117921949

Start: 4212053

End: 4212955

Strand: Direct

Name: dmlR [H]

Synonym: Shewana3_3512

Alternate gene names: 117921949

Gene position: 4212053-4212955 (Clockwise)

Preceding gene: 117921947

Following gene: 117921955

Centisome position: 84.71

GC content: 54.37

Gene sequence:

>903_bases
ATGGATAAAATCGCCGCCATGCGCAGCTTTATCGAAGTGGCCAACTGTGGCAGCTTTACTAAGGCCGCAGAACACCTCGA
TCTCAGTCGCCTACAGGTATCACGTCATATTCAAGAAATTGAACAGTGGCTGAGTTTACGTTTATTGCACCGCACGACCC
GCAGCGTCAGCCTCACTCTGCAGGGGGAAGAAGCCCTGCAATATTGTCAGCGGGTGCTGAGCGAAGTCGCCGCAATGGAA
AGCCGCGCCCATAGTCATAACACCGAATTAGTTGGCAGTATTCGCATCGCTACGCCCGTCGGGCTTGGGCAGCACAGTCT
GTTTGATGTGGTCGATCGCTTTATAAAATTGCACCCTAAGGTCAATATTCAGCTGTTAATGTCGGACAGTTTTGCCCAGT
TGGTGGATGAGCGGGTGGACGTGGCACTGCGCTATACCGAGCAGCCCGATGAGAATTTGATTGCCCGCAAACTGATGAGC
ATAGATGCCGTGCTGTGCGCCGCGCCCAGTTACTTGGCTCAAGCCGAGCCGCTAACCGTCCCTAACGATCTGCTCAGCCA
TAATTGCCTTATCCATAGCTCGCAGCAACATTGGCAGCTCCTCAAGGAGCAACAGAGTGAGCTGATTAAACCTATGGGGA
ATCTGATGGCGAATGAAATGGGAGTGCTGGTCAGCGCCGCATTGCGCGGGCATGGCATTGCCTACTTGCCCTGCGACTTA
GCCAATCCCTATCTGGCCAGCGGCCAACTGCAAGAAGTGCTCCCCGAATACACGGCGCCAGGGCAAACCCTCTGGGCTGT
GTATCTCTCCCGCAGTTATCAACAAGCGCTGGTGCGCGCCTTTATCGATTTTACCGCCCAGCAATGGCAGCAGGATCTCA
GCAAGTGGACGACGGCGAGTTAA

Upstream 100 bases:

>100_bases
ATAAGTCTCGCTACTGATTTAAATTGAGACCAGTTTACTTGCGACACTTCACGTGATAAATATCGATAAATGAGCATGAT
TGTTTCAGATTGAGATACTA

Downstream 100 bases:

>100_bases
GCCCACAAACAAAAACGCACTGAGGCTTTAAGGCTCAGTGCGTTGAGTCGCGGTTTGCGAAGGCTTAATTATCGAACTGC
ATCAAAAAATCTATACTTGG

Product: transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDKIAAMRSFIEVANCGSFTKAAEHLDLSRLQVSRHIQEIEQWLSLRLLHRTTRSVSLTLQGEEALQYCQRVLSEVAAME
SRAHSHNTELVGSIRIATPVGLGQHSLFDVVDRFIKLHPKVNIQLLMSDSFAQLVDERVDVALRYTEQPDENLIARKLMS
IDAVLCAAPSYLAQAEPLTVPNDLLSHNCLIHSSQQHWQLLKEQQSELIKPMGNLMANEMGVLVSAALRGHGIAYLPCDL
ANPYLASGQLQEVLPEYTAPGQTLWAVYLSRSYQQALVRAFIDFTAQQWQQDLSKWTTAS

Sequences:

>Translated_300_residues
MDKIAAMRSFIEVANCGSFTKAAEHLDLSRLQVSRHIQEIEQWLSLRLLHRTTRSVSLTLQGEEALQYCQRVLSEVAAME
SRAHSHNTELVGSIRIATPVGLGQHSLFDVVDRFIKLHPKVNIQLLMSDSFAQLVDERVDVALRYTEQPDENLIARKLMS
IDAVLCAAPSYLAQAEPLTVPNDLLSHNCLIHSSQQHWQLLKEQQSELIKPMGNLMANEMGVLVSAALRGHGIAYLPCDL
ANPYLASGQLQEVLPEYTAPGQTLWAVYLSRSYQQALVRAFIDFTAQQWQQDLSKWTTAS
>Mature_300_residues
MDKIAAMRSFIEVANCGSFTKAAEHLDLSRLQVSRHIQEIEQWLSLRLLHRTTRSVSLTLQGEEALQYCQRVLSEVAAME
SRAHSHNTELVGSIRIATPVGLGQHSLFDVVDRFIKLHPKVNIQLLMSDSFAQLVDERVDVALRYTEQPDENLIARKLMS
IDAVLCAAPSYLAQAEPLTVPNDLLSHNCLIHSSQQHWQLLKEQQSELIKPMGNLMANEMGVLVSAALRGHGIAYLPCDL
ANPYLASGQLQEVLPEYTAPGQTLWAVYLSRSYQQALVRAFIDFTAQQWQQDLSKWTTAS

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=33.5640138408304, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=31.8791946308725, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789639, Length=274, Percent_Identity=30.2919708029197, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=32.9861111111111, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=28.3276450511945, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=30.2405498281787, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787128, Length=281, Percent_Identity=27.7580071174377, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789173, Length=246, Percent_Identity=24.7967479674797, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=144, Percent_Identity=29.8611111111111, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=23.469387755102, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790011, Length=289, Percent_Identity=23.8754325259516, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786713, Length=269, Percent_Identity=26.7657992565056, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=32.7586206896552, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33660; Mature: 33660

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIAAMRSFIEVANCGSFTKAAEHLDLSRLQVSRHIQEIEQWLSLRLLHRTTRSVSLTL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
QGEEALQYCQRVLSEVAAMESRAHSHNTELVGSIRIATPVGLGQHSLFDVVDRFIKLHPK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
VNIQLLMSDSFAQLVDERVDVALRYTEQPDENLIARKLMSIDAVLCAAPSYLAQAEPLTV
CEEEEEECCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
PNDLLSHNCLIHSSQQHWQLLKEQQSELIKPMGNLMANEMGVLVSAALRGHGIAYLPCDL
CHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ANPYLASGQLQEVLPEYTAPGQTLWAVYLSRSYQQALVRAFIDFTAQQWQQDLSKWTTAS
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKIAAMRSFIEVANCGSFTKAAEHLDLSRLQVSRHIQEIEQWLSLRLLHRTTRSVSLTL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
QGEEALQYCQRVLSEVAAMESRAHSHNTELVGSIRIATPVGLGQHSLFDVVDRFIKLHPK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
VNIQLLMSDSFAQLVDERVDVALRYTEQPDENLIARKLMSIDAVLCAAPSYLAQAEPLTV
CEEEEEECCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
PNDLLSHNCLIHSSQQHWQLLKEQQSELIKPMGNLMANEMGVLVSAALRGHGIAYLPCDL
CHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ANPYLASGQLQEVLPEYTAPGQTLWAVYLSRSYQQALVRAFIDFTAQQWQQDLSKWTTAS
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]