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The map label for this gene is est [H]

Identifier: 117921923

GI number: 117921923

Start: 4180632

End: 4181546

Strand: Direct

Name: est [H]

Synonym: Shewana3_3486

Alternate gene names: 117921923

Gene position: 4180632-4181546 (Clockwise)

Preceding gene: 117921921

Following gene: 117921925

Centisome position: 84.08

GC content: 53.22

Gene sequence:

>915_bases
ATGAGCCAACTCTATTTAGAAGACGGTATTAAACAATTAGCGAGCGAGTTTATCGCCGCAGGTTGCCCTTCGGTGCGCGA
ACAGAGCCTTGAGGAGCGACGCCAAGGGTATATCGCCAGCACAGTATTGGCAGGTGAGCCGGAAGCTGTGTTTGAAGTTA
AAACCCTATCCCTTGAGGGCATCGAATTAACGCTATTTAAACCCTCGGTGGACAACAACTTACCCGTGGTCATTTACTAT
CACGGCGGCTGCTTTGTCAGTGGCGGCATCGCCACCCACAATCAACAACTGCGTAAAATCGCCAATGATTCCGGCGCGTT
AGTGGTAGCGGTCAGCTATCGTCTCGCCCCAGAGCATGTGTACCCAGCAGCCCATGACGATGCCTTCAATGCCGCCAATC
TCGTGCAACAACATTGCCAGCAATGGGGCGGTGATGACACCAATATCACCCTGATGGGCGACAGTGCTGGCGGCCATTTA
GCCTTAGTCACTTGCCTGCGGTTAAAGGCACAAGGGCAATGGTTACCTAAAAAACAAGTGCTTATCTATCCGATGTTGGA
TGCGACCGCCAAGAGTCAGAGCTATATCGACAATGGCGACAAGTACATTATCACCCGCGATACGCTGCTCACGGGCTTTG
ATATGTACCTCGACTGGCACCCCAGAACCGACGTTGAAGCCAGCCCACTGCGCAGCCACGACCTAGCCGGTTTACCCGAA
ACCCACATTATTACCGCCGAGTTCGATCCGCTGTTAGATGAGGGCGAACAGCTGTTCCGTAACCTATTAGATGCGGGCGT
CAATGCCCATTGCCGCCGCTATTTAGGGGTGATCCACGGCTTCTTCCAACTCGCTGGCGTCAGCCAATCGGCGCGGGATG
CCATGGTGCAGATAACCCACATTATTCGCGTTTAA

Upstream 100 bases:

>100_bases
ATCGTCCAATACGCACTACCCTTTTCAGCATCGAGCCGATACACTTTGCCGACCACAAGGGGCGATAAGCGCTCACCGAA
TATATTCAGGGAAACTGCAC

Downstream 100 bases:

>100_bases
CCTCAGTAAGCCGCATGCCTTAATGCGGGGCATGCGGCGATTGAATCCATTCAACGAATAACCTATTGCATGATAACGCA
ATGCTCTACTGCGCTATTTC

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSQLYLEDGIKQLASEFIAAGCPSVREQSLEERRQGYIASTVLAGEPEAVFEVKTLSLEGIELTLFKPSVDNNLPVVIYY
HGGCFVSGGIATHNQQLRKIANDSGALVVAVSYRLAPEHVYPAAHDDAFNAANLVQQHCQQWGGDDTNITLMGDSAGGHL
ALVTCLRLKAQGQWLPKKQVLIYPMLDATAKSQSYIDNGDKYIITRDTLLTGFDMYLDWHPRTDVEASPLRSHDLAGLPE
THIITAEFDPLLDEGEQLFRNLLDAGVNAHCRRYLGVIHGFFQLAGVSQSARDAMVQITHIIRV

Sequences:

>Translated_304_residues
MSQLYLEDGIKQLASEFIAAGCPSVREQSLEERRQGYIASTVLAGEPEAVFEVKTLSLEGIELTLFKPSVDNNLPVVIYY
HGGCFVSGGIATHNQQLRKIANDSGALVVAVSYRLAPEHVYPAAHDDAFNAANLVQQHCQQWGGDDTNITLMGDSAGGHL
ALVTCLRLKAQGQWLPKKQVLIYPMLDATAKSQSYIDNGDKYIITRDTLLTGFDMYLDWHPRTDVEASPLRSHDLAGLPE
THIITAEFDPLLDEGEQLFRNLLDAGVNAHCRRYLGVIHGFFQLAGVSQSARDAMVQITHIIRV
>Mature_303_residues
SQLYLEDGIKQLASEFIAAGCPSVREQSLEERRQGYIASTVLAGEPEAVFEVKTLSLEGIELTLFKPSVDNNLPVVIYYH
GGCFVSGGIATHNQQLRKIANDSGALVVAVSYRLAPEHVYPAAHDDAFNAANLVQQHCQQWGGDDTNITLMGDSAGGHLA
LVTCLRLKAQGQWLPKKQVLIYPMLDATAKSQSYIDNGDKYIITRDTLLTGFDMYLDWHPRTDVEASPLRSHDLAGLPET
HIITAEFDPLLDEGEQLFRNLLDAGVNAHCRRYLGVIHGFFQLAGVSQSARDAMVQITHIIRV

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=282, Percent_Identity=28.7234042553192, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI21328446, Length=109, Percent_Identity=34.8623853211009, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI61966717, Length=315, Percent_Identity=25.0793650793651, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI157041239, Length=286, Percent_Identity=24.4755244755245, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1786682, Length=282, Percent_Identity=26.9503546099291, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=141, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=150, Percent_Identity=32, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=123, Percent_Identity=34.9593495934959, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17567059, Length=208, Percent_Identity=25, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33488; Mature: 33357

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLYLEDGIKQLASEFIAAGCPSVREQSLEERRQGYIASTVLAGEPEAVFEVKTLSLEG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECC
IELTLFKPSVDNNLPVVIYYHGGCFVSGGIATHNQQLRKIANDSGALVVAVSYRLAPEHV
EEEEEECCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCC
YPAAHDDAFNAANLVQQHCQQWGGDDTNITLMGDSAGGHLALVTCLRLKAQGQWLPKKQV
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCE
LIYPMLDATAKSQSYIDNGDKYIITRDTLLTGFDMYLDWHPRTDVEASPLRSHDLAGLPE
EEEEECCCCCCCCHHHCCCCEEEEEECHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
THIITAEFDPLLDEGEQLFRNLLDAGVNAHCRRYLGVIHGFFQLAGVSQSARDAMVQITH
CEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IIRV
HHCC
>Mature Secondary Structure 
SQLYLEDGIKQLASEFIAAGCPSVREQSLEERRQGYIASTVLAGEPEAVFEVKTLSLEG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECC
IELTLFKPSVDNNLPVVIYYHGGCFVSGGIATHNQQLRKIANDSGALVVAVSYRLAPEHV
EEEEEECCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCC
YPAAHDDAFNAANLVQQHCQQWGGDDTNITLMGDSAGGHLALVTCLRLKAQGQWLPKKQV
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCE
LIYPMLDATAKSQSYIDNGDKYIITRDTLLTGFDMYLDWHPRTDVEASPLRSHDLAGLPE
EEEEECCCCCCCCHHHCCCCEEEEEECHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
THIITAEFDPLLDEGEQLFRNLLDAGVNAHCRRYLGVIHGFFQLAGVSQSARDAMVQITH
CEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IIRV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]