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The map label for this gene is ygfZ [C]

Identifier: 117921854

GI number: 117921854

Start: 4087578

End: 4088534

Strand: Direct

Name: ygfZ [C]

Synonym: Shewana3_3417

Alternate gene names: 117921854

Gene position: 4087578-4088534 (Clockwise)

Preceding gene: 117921853

Following gene: 117921858

Centisome position: 82.21

GC content: 51.83

Gene sequence:

>957_bases
ATGACTATTGCAGTTTCGACACCCACTTGGGATCTGGACGCCTCCATGCATCCACTTGTGCTCGCCAACCTATCCCACCT
CGGCCTCATCAAAGTAGTCGGTGAACAGGGGCGCAGTTTTATCCATGGCCAAGTGACTACGGACATTAGCTCACTCGAAG
CCAACCAATGGCGCTGGGGCGCACATTGCGATCCTAAGGGTAAGATGATCGCCAGCTTCCGCACCTTTGCTATCCAAGAG
GCGCTGTTGATGCTGCTGCCAAGGGAGACTATCGAGGTCGATCTGCCGCAACTGCAAAAATATGCGGTGTTTAGCAAGGC
AACCCTGACCAATGCGACGGCAGAGTGGACTTTACTCGGCGTAGCAGGCGAACAAGCAACACAATTTGTGACCCAACACT
TTGGGGAAATCACTGAAGAACTGACGCTGGTCGAACATGGCGCCATTTTAAAGGATGCGGATCGTTTTATTCTGGTTCTG
CAGCCAGAGGCGGCAACTACCTTAGTGGGCGAGCACACAGTGTTTGATGCCAGCGCTTGGCAAGCCTTAGAAATTACAGC
AGGCTACCCTAACCTAGCGCCAAGCCATGCTAATCAATATGTGCCGCAGATGTGTAACCTACAGGCTATCAACGGCATCA
GTTTTAACAAAGGCTGCTACATGGGCCAAGAAACTGTGGCTCGGATGAAATACCGTGGCGGTAACAAGCGCGCGCTCTAT
ATTCTCCACGGCACGACTTCCTCAGACATCAATTTGGAAAGTGCTCTCGAGATCGAATTGGAAGATGGCTATCGCAAGGG
CGGGCAAATTATTGAGTTCGTACAGCGTGGTAATCAAGTACTGCTCACCGCTGTGCTGGCAAACGATACCGCGAACGATG
CCAAGCTGCGCTTTGCCGACGATGAGCAATCCAGCTTACACATTAAAGCATTACCCTACTCGCTCGAAGAAGCCTAG

Upstream 100 bases:

>100_bases
CACATTTCATTCAAGATATGTGCGCCTTATGCTTTTGAGCGCCGCTTAATTCCGCTACAATCTAGCCTCTTGCATCGTCT
TCATTTTCAGGATCTCCGCT

Downstream 100 bases:

>100_bases
CTTCTAAACGAGTCGTATTGAAAAAGGCGCCCTAGGCGCCTTTTGCTTTTAATGCTCGATATAAGTATCTCGAATCGCCA
CCACCTCGGGCAGAATTTGC

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTIAVSTPTWDLDASMHPLVLANLSHLGLIKVVGEQGRSFIHGQVTTDISSLEANQWRWGAHCDPKGKMIASFRTFAIQE
ALLMLLPRETIEVDLPQLQKYAVFSKATLTNATAEWTLLGVAGEQATQFVTQHFGEITEELTLVEHGAILKDADRFILVL
QPEAATTLVGEHTVFDASAWQALEITAGYPNLAPSHANQYVPQMCNLQAINGISFNKGCYMGQETVARMKYRGGNKRALY
ILHGTTSSDINLESALEIELEDGYRKGGQIIEFVQRGNQVLLTAVLANDTANDAKLRFADDEQSSLHIKALPYSLEEA

Sequences:

>Translated_318_residues
MTIAVSTPTWDLDASMHPLVLANLSHLGLIKVVGEQGRSFIHGQVTTDISSLEANQWRWGAHCDPKGKMIASFRTFAIQE
ALLMLLPRETIEVDLPQLQKYAVFSKATLTNATAEWTLLGVAGEQATQFVTQHFGEITEELTLVEHGAILKDADRFILVL
QPEAATTLVGEHTVFDASAWQALEITAGYPNLAPSHANQYVPQMCNLQAINGISFNKGCYMGQETVARMKYRGGNKRALY
ILHGTTSSDINLESALEIELEDGYRKGGQIIEFVQRGNQVLLTAVLANDTANDAKLRFADDEQSSLHIKALPYSLEEA
>Mature_317_residues
TIAVSTPTWDLDASMHPLVLANLSHLGLIKVVGEQGRSFIHGQVTTDISSLEANQWRWGAHCDPKGKMIASFRTFAIQEA
LLMLLPRETIEVDLPQLQKYAVFSKATLTNATAEWTLLGVAGEQATQFVTQHFGEITEELTLVEHGAILKDADRFILVLQ
PEAATTLVGEHTVFDASAWQALEITAGYPNLAPSHANQYVPQMCNLQAINGISFNKGCYMGQETVARMKYRGGNKRALYI
LHGTTSSDINLESALEIELEDGYRKGGQIIEFVQRGNQVLLTAVLANDTANDAKLRFADDEQSSLHIKALPYSLEEA

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=311, Percent_Identity=38.9067524115756, Blast_Score=198, Evalue=5e-52,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 34970; Mature: 34839

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAVSTPTWDLDASMHPLVLANLSHLGLIKVVGEQGRSFIHGQVTTDISSLEANQWRWG
CEEEEECCCCCCCCCCCCCEEECCCCCEEEEEECCCCCEEEECEEECCHHHCCCCCCEEC
AHCDPKGKMIASFRTFAIQEALLMLLPRETIEVDLPQLQKYAVFSKATLTNATAEWTLLG
CCCCCCCCEEHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCEEEEEE
VAGEQATQFVTQHFGEITEELTLVEHGAILKDADRFILVLQPEAATTLVGEHTVFDASAW
ECCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCEEEECCCEEEECCCC
QALEITAGYPNLAPSHANQYVPQMCNLQAINGISFNKGCYMGQETVARMKYRGGNKRALY
EEEEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
ILHGTTSSDINLESALEIELEDGYRKGGQIIEFVQRGNQVLLTAVLANDTANDAKLRFAD
EEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEC
DEQSSLHIKALPYSLEEA
CCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TIAVSTPTWDLDASMHPLVLANLSHLGLIKVVGEQGRSFIHGQVTTDISSLEANQWRWG
EEEEECCCCCCCCCCCCCEEECCCCCEEEEEECCCCCEEEECEEECCHHHCCCCCCEEC
AHCDPKGKMIASFRTFAIQEALLMLLPRETIEVDLPQLQKYAVFSKATLTNATAEWTLLG
CCCCCCCCEEHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCEEEEEE
VAGEQATQFVTQHFGEITEELTLVEHGAILKDADRFILVLQPEAATTLVGEHTVFDASAW
ECCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCEEEECCCEEEECCCC
QALEITAGYPNLAPSHANQYVPQMCNLQAINGISFNKGCYMGQETVARMKYRGGNKRALY
EEEEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEE
ILHGTTSSDINLESALEIELEDGYRKGGQIIEFVQRGNQVLLTAVLANDTANDAKLRFAD
EEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEC
DEQSSLHIKALPYSLEEA
CCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]