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The map label for this gene is metK [H]

Identifier: 117921792

GI number: 117921792

Start: 4025097

End: 4026248

Strand: Direct

Name: metK [H]

Synonym: Shewana3_3354

Alternate gene names: 117921792

Gene position: 4025097-4026248 (Clockwise)

Preceding gene: 117921783

Following gene: 117921793

Centisome position: 80.95

GC content: 49.74

Gene sequence:

>1152_bases
ATGGCAAAGCACTTGTTTACTTCTGAGTCGGTCTCAGAAGGTCATCCCGATAAGATTGCAGATCAGATTTCTGATGCTGT
GCTAGATGCGATTCTGGCTCAAGATCCTAAAGCACGTGTTGCGTGCGAAACATATGTCAAAACTGGCATGGTATTAGTAG
GTGGCGAAGTGACCACCTCTGCCTGGGTTGATATTGAAGAGCTGACCCGTAAAACGGTTCGTGAAATTGGTTATGTCCAT
TCAGATATGGGCTTCGATGCCGATTCTTGCGCAGTATTAAATGCGATTGGTAAACAGTCTCCAGACATCAACCAAGGTGT
CGACCGTGCCGATCCAAAAGAACAAGGTGCCGGCGACCAAGGTTTAATGTTTGGTTATGCCAGCAACGAAACTGACATTC
TGATGCCAGCGCCTATCACCTATGCCCACGCATTAGTGAAGCGTCAATCAGAAGTGCGTAAAGATGGCACTCTGCCATGG
TTACGCCCAGATGCGAAAAGCCAAGTCACCTTCGCTTACGAAGACAACAAAATTGTTGGTATCGATGCGATCGTACTGTC
TACCCAACATAGCCCTGACATCGCCCAAGCGGATCTGATCGAAGGCGTGATGGAAACCATCATCAAGCCAGTTCTGCCAG
CACAATGGCTGAACAAGGACACTAAATACTTCATCAACCCAACTGGCCGTTTCGTTATCGGTGGTCCAATGGGTGACTGT
GGTTTAACCGGTCGTAAGATCATCGTAGACACCTACGGTGGTATGGCGCGTCACGGTGGTGGTGCTTTCTCTGGTAAAGA
CCCATCAAAAGTTGACCGTAGTGCGGCTTACGCTGCCCGTTACGTAGCGAAAAACATCGTTGCAGCAGGCTTAGCGGACC
GTTGTGAGATCCAAGTGTCTTACGCTATCGGTGTGGCTGAACCTACGTCTATCAGCGTTGAGACCTTCGGTACTGGTAAA
GTATCAGAAGAAGTCTTAATCAAGTTAGTCCGTCAACACTTCGACCTGCGCCCATACGGCCTGACCGAAATGTTGAACCT
GGCTCGCCCAATCTATCAAGCAACCGCCGCTTATGGTCACTTTGGCCGTAACGAGTTCCCATGGGAAGCGACGGATAAAG
CTGACGCACTGCGCGCCGATGCAGGTCTGTAA

Upstream 100 bases:

>100_bases
TTAGTTTAGGGGGTGTCAACCAAGGGAAATTCCACTAAAATAGCCGTCTAGATGTAGATGCTTCTACACGTTTGATTGCT
AACGACGAGATTTTCCTACT

Downstream 100 bases:

>100_bases
TCTGCATTAAGCTCACGCTTTCGCTTGTAAAAAACCGCCTAAGGGCGGTTTCTTTTATGCTTAAGATGTATTGCCTAACC
CCATCATAATCAGCAAGATG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTSAWVDIEELTRKTVREIGYVH
SDMGFDADSCAVLNAIGKQSPDINQGVDRADPKEQGAGDQGLMFGYASNETDILMPAPITYAHALVKRQSEVRKDGTLPW
LRPDAKSQVTFAYEDNKIVGIDAIVLSTQHSPDIAQADLIEGVMETIIKPVLPAQWLNKDTKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISVETFGTGK
VSEEVLIKLVRQHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEATDKADALRADAGL

Sequences:

>Translated_383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTSAWVDIEELTRKTVREIGYVH
SDMGFDADSCAVLNAIGKQSPDINQGVDRADPKEQGAGDQGLMFGYASNETDILMPAPITYAHALVKRQSEVRKDGTLPW
LRPDAKSQVTFAYEDNKIVGIDAIVLSTQHSPDIAQADLIEGVMETIIKPVLPAQWLNKDTKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISVETFGTGK
VSEEVLIKLVRQHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEATDKADALRADAGL
>Mature_382_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTSAWVDIEELTRKTVREIGYVHS
DMGFDADSCAVLNAIGKQSPDINQGVDRADPKEQGAGDQGLMFGYASNETDILMPAPITYAHALVKRQSEVRKDGTLPWL
RPDAKSQVTFAYEDNKIVGIDAIVLSTQHSPDIAQADLIEGVMETIIKPVLPAQWLNKDTKYFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISVETFGTGKV
SEEVLIKLVRQHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEATDKADALRADAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=375, Percent_Identity=58.6666666666667, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=57.3333333333333, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=85.9007832898172, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=372, Percent_Identity=57.258064516129, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17538494, Length=372, Percent_Identity=57.258064516129, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=55.699481865285, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=54.9597855227882, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565905, Length=372, Percent_Identity=51.6129032258064, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI32565911, Length=325, Percent_Identity=53.5384615384615, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI25145635, Length=339, Percent_Identity=51.9174041297935, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI32565903, Length=298, Percent_Identity=53.6912751677852, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=62.0689655172414, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=76.0869565217391, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320710, Length=374, Percent_Identity=56.4171122994652, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=57.027027027027, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=56.1497326203209, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=56.1497326203209, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=56.1497326203209, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=56.1497326203209, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=56.1497326203209, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=56.1497326203209, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=55.2278820375335, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=55.2278820375335, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=372, Percent_Identity=52.9569892473118, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=56.6666666666667, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41366; Mature: 41235

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEELTRKTVREIGYVHSDMGFDADSCAVLNAIGKQSPDINQGVDRADPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYASNETDILMPAPITYAHALVKRQSEVRKDGTLPWLRPDAKSQVTFAYEDNKIVG
CEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEE
IDAIVLSTQHSPDIAQADLIEGVMETIIKPVLPAQWLNKDTKYFINPTGRFVIGGPMGDC
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISVETFGTGKVSEEVLIKLVRQHFDLRPYGLTEMLNLARPIYQATAAYGH
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
FGRNEFPWEATDKADALRADAGL
CCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILAQDPKARVACETYVKTGMVLVGGEVTTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEELTRKTVREIGYVHSDMGFDADSCAVLNAIGKQSPDINQGVDRADPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYASNETDILMPAPITYAHALVKRQSEVRKDGTLPWLRPDAKSQVTFAYEDNKIVG
CEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEE
IDAIVLSTQHSPDIAQADLIEGVMETIIKPVLPAQWLNKDTKYFINPTGRFVIGGPMGDC
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISVETFGTGKVSEEVLIKLVRQHFDLRPYGLTEMLNLARPIYQATAAYGH
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
FGRNEFPWEATDKADALRADAGL
CCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA