Click here to switch to the map view.

The map label for this gene is ahpF [H]

Identifier: 117921764

GI number: 117921764

Start: 3987431

End: 3989008

Strand: Direct

Name: ahpF [H]

Synonym: Shewana3_3326

Alternate gene names: 117921764

Gene position: 3987431-3989008 (Clockwise)

Preceding gene: 117921763

Following gene: 117921771

Centisome position: 80.19

GC content: 49.68

Gene sequence:

>1578_bases
ATGTTAGATGCGAATTTAAAAAATCAACTGCAAACCTATCTGCAAAACCTCAAGAGACCAGTTGAACTTGTCGTGTCAGC
AGATGAAAGCAAGAAGTCTCAAGAATTAAAAAGCTTAGCCAATGACATCGTTAGCCTTTCCTCTTTAGTGAGCCTCAAAG
AAGCTCAAGGCTCTCGTACGCCAGCGATGACAGTGGTTAACACCGATCTCAATACTCAGATTAGCTTTGCTGGTCTGCCT
ATGGGTCACGAATTCACCTCACTCGTGTTAGCCCTGCTGCACACGGGTGGACACCCAATCAAACTCAGCGACGAAGTCAT
TGAGCAGATCCGTAATCTGCCTGGCAAATATGAGTTTGAAACCTATGTTTCACTCACCTGCCAAAACTGCCCTGATGTGA
TTCAGGCGCTAAATATGATGGCTGCGATTAACCCGAACATCACCAACGTGATGATCGACGGCGCCCTGTTCCAAGACGAA
GTGGCGAGTCGTAATATCATGGCCGTACCGTCTGTGTACTTAAACGGTGAAGTGTTTGCCGCAGGCCGTATCAGCATCGG
TGAGATCTTAAATAAACTCGATACTGGCGCTGCCAGCCGTAAAGCTGAAGAACTCAGCCAAAAAGCCCCATACGAAGTCT
TAGTCGTCGGTGGTGGCCCGGCTGGCGCAGCTGCCGCGATTTACGCTGCCCGTAAAGGTCTTCGCACTGGTGTGGTTGCA
GATAAATTTGGTGGCCAAGTTGCCGAAACTGTAGGTATCGAAAACTTTATTTCGGTAAAAGCGACTGAAGGTCCAAAACT
GGTGGCTAACCTTGAAGCCCACGTTCGTGACTACGAAGTCGACATCATGGATAACCAGAAAGCCGTTAAACTGGCGAGCG
ATGGATTATTCGAACTCGAACTGGCAAGCGGTGCAACACTGCGTAGCCGTACCATCCTTTTGGCAACCGGTGCCCGCTGG
CGCGAAATGAATGTCCCCGGTGAGAAAGAATACCGTGGTAAGGGCGTAGCTTACTGCCCACACTGTGACGGCCCATTATT
TAAAGGCAAACGTGTTGCAGTGATCGGTGGCGGTAACTCAGGTATCGAAGCGGCAATCGATTTAGCCAACATTGTTGAGC
ATGTGACCGTACTCGAATTTGATAGCAAACTGCGCGCCGATGACGTACTGCAACGTAAAGCGGCCTCTATGGGTAATATC
CATATCATTACTCAAGCGATGACCACTGAAGTGACTGGTGACGGTACCCGTGTAAACGGCCTGAACTACACCGACCGTGC
AACGGGTGAAAGCCACCATATCGCGCTGGCTGGTATCTTCGTACAAATCGGTTTAGTGCCCAATGCCGAATGGTTAAAAG
GCACTGTGGATTTAACTCCTCGTGGCGAAATCATCGTCGATGAGCGCGGCCAAACCTCTATCCCAGGTGTATTCGCCGCT
GGTGACGTGACTAACTCACCTTACAAGCAAATCATCATCGCTATGGGTAGCGGTGCCAATGCTTCACTGGGTGCGTTTGA
TTACCTCATTCGCCATAGCGATGATAGTGCCGAGACCACAGAAACTAAAGCGGCTTAA

Upstream 100 bases:

>100_bases
CTGCGGTGCCAAATAGGAAGAGAGAACCGCCCCCTGCAAGGTTCTAGGTTGGCCCCCTCGCGTTTTCAATTATTTCATTT
TCAAGGATTTTGGAGTCATC

Downstream 100 bases:

>100_bases
TCGTTAACGATTATTCCCCTCACGAAAAAGCCAGCTGATGCTGGCTTTTTGTTTTAGTGTTAACAATCCTGTTCTACAGG
TAAGCATTCTCAAGCCTAAT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLANDIVSLSSLVSLKEAQGSRTPAMTVVNTDLNTQISFAGLP
MGHEFTSLVLALLHTGGHPIKLSDEVIEQIRNLPGKYEFETYVSLTCQNCPDVIQALNMMAAINPNITNVMIDGALFQDE
VASRNIMAVPSVYLNGEVFAAGRISIGEILNKLDTGAASRKAEELSQKAPYEVLVVGGGPAGAAAAIYAARKGLRTGVVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVKLASDGLFELELASGATLRSRTILLATGARW
REMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSKLRADDVLQRKAASMGNI
HIITQAMTTEVTGDGTRVNGLNYTDRATGESHHIALAGIFVQIGLVPNAEWLKGTVDLTPRGEIIVDERGQTSIPGVFAA
GDVTNSPYKQIIIAMGSGANASLGAFDYLIRHSDDSAETTETKAA

Sequences:

>Translated_525_residues
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLANDIVSLSSLVSLKEAQGSRTPAMTVVNTDLNTQISFAGLP
MGHEFTSLVLALLHTGGHPIKLSDEVIEQIRNLPGKYEFETYVSLTCQNCPDVIQALNMMAAINPNITNVMIDGALFQDE
VASRNIMAVPSVYLNGEVFAAGRISIGEILNKLDTGAASRKAEELSQKAPYEVLVVGGGPAGAAAAIYAARKGLRTGVVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVKLASDGLFELELASGATLRSRTILLATGARW
REMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSKLRADDVLQRKAASMGNI
HIITQAMTTEVTGDGTRVNGLNYTDRATGESHHIALAGIFVQIGLVPNAEWLKGTVDLTPRGEIIVDERGQTSIPGVFAA
GDVTNSPYKQIIIAMGSGANASLGAFDYLIRHSDDSAETTETKAA
>Mature_525_residues
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLANDIVSLSSLVSLKEAQGSRTPAMTVVNTDLNTQISFAGLP
MGHEFTSLVLALLHTGGHPIKLSDEVIEQIRNLPGKYEFETYVSLTCQNCPDVIQALNMMAAINPNITNVMIDGALFQDE
VASRNIMAVPSVYLNGEVFAAGRISIGEILNKLDTGAASRKAEELSQKAPYEVLVVGGGPAGAAAAIYAARKGLRTGVVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVKLASDGLFELELASGATLRSRTILLATGARW
REMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSKLRADDVLQRKAASMGNI
HIITQAMTTEVTGDGTRVNGLNYTDRATGESHHIALAGIFVQIGLVPNAEWLKGTVDLTPRGEIIVDERGQTSIPGVFAA
GDVTNSPYKQIIIAMGSGANASLGAFDYLIRHSDDSAETTETKAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=58.6073500967118, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=35.4838709677419, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=34.7266881028939, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=337, Percent_Identity=32.6409495548961, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55972; Mature: 55972

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLANDIVSLSSLVSLKEAQGSRT
CCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAMTVVNTDLNTQISFAGLPMGHEFTSLVLALLHTGGHPIKLSDEVIEQIRNLPGKYEFE
CEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHCCCCCEEEE
TYVSLTCQNCPDVIQALNMMAAINPNITNVMIDGALFQDEVASRNIMAVPSVYLNGEVFA
EEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCEEECCEEEECCEEEE
AGRISIGEILNKLDTGAASRKAEELSQKAPYEVLVVGGGPAGAAAAIYAARKGLRTGVVA
ECCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCH
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVKLASDGLFELE
HHCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHCEEEEEEEECCCCEEEEECCCEEEEE
LASGATLRSRTILLATGARWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNS
ECCCCEEECCEEEEEECCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDSKLRADDVLQRKAASMGNIHIITQAMTTEVTGDGTRVNG
CHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEECC
LNYTDRATGESHHIALAGIFVQIGLVPNAEWLKGTVDLTPRGEIIVDERGQTSIPGVFAA
CCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHEEEEEECCCCCEEEECCCCCCCCCEEEE
GDVTNSPYKQIIIAMGSGANASLGAFDYLIRHSDDSAETTETKAA
CCCCCCCCEEEEEEECCCCCCCHHHHHHHEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLANDIVSLSSLVSLKEAQGSRT
CCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAMTVVNTDLNTQISFAGLPMGHEFTSLVLALLHTGGHPIKLSDEVIEQIRNLPGKYEFE
CEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHCCCCCEEEE
TYVSLTCQNCPDVIQALNMMAAINPNITNVMIDGALFQDEVASRNIMAVPSVYLNGEVFA
EEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCEEECCEEEECCEEEE
AGRISIGEILNKLDTGAASRKAEELSQKAPYEVLVVGGGPAGAAAAIYAARKGLRTGVVA
ECCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCH
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVKLASDGLFELE
HHCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHCEEEEEEEECCCCEEEEECCCEEEEE
LASGATLRSRTILLATGARWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNS
ECCCCEEECCEEEEEECCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDSKLRADDVLQRKAASMGNIHIITQAMTTEVTGDGTRVNG
CHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEECC
LNYTDRATGESHHIALAGIFVQIGLVPNAEWLKGTVDLTPRGEIIVDERGQTSIPGVFAA
CCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHEEEEEECCCCCEEEECCCCCCCCCEEEE
GDVTNSPYKQIIIAMGSGANASLGAFDYLIRHSDDSAETTETKAA
CCCCCCCCEEEEEEECCCCCCCHHHHHHHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]