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The map label for this gene is cobQ [H]

Identifier: 117921713

GI number: 117921713

Start: 3914494

End: 3916119

Strand: Reverse

Name: cobQ [H]

Synonym: Shewana3_3275

Alternate gene names: 117921713

Gene position: 3916119-3914494 (Counterclockwise)

Preceding gene: 117921714

Following gene: 117921712

Centisome position: 78.76

GC content: 52.21

Gene sequence:

>1626_bases
ATGAATTCAAATAACGCCTCTAATCCTACTTCGGCCTTGTCGCAAAACCTGTTTCAAGGTGTTGTAAGCCAAAGTGAAGC
TCAGCATTCGGGCGCAAAAGTGCTAATGGTGCAGGGGACTACCTCGGATGCGGGCAAAAGTACGCTAGTGGCGGGGATTT
GTCGATTGCTCGCCCGTGAGGGCGTTAAGGTCGCGCCTTTTAAGCCACAGAATATGGCGCTTAACAGCGCAGTCACCATT
GATGGCGGGGAGATTGGCCGCGCTCAGGCACTGCAAGCCGCAGCCTGTTTTCTCGCGCCGCACACGGATTTTAATCCCAT
CCTGCTTAAGCCAAGTTCGGACACGGGCGCGCAGATCATTGTGCACGGCAAAGCCTTGACCACCTTAGAGGCCTCGGCCT
TTTTCGGGGAAAAATCTAAGGATTATAAAGCCATGGCGCTAAATGCCGTGCTGGAATCCTTTGCGCGTTTAGGTCAGCAA
TACCAAACGATTGTGGTTGAAGGGGCGGGGAGCCCGGCGGAAATTAATCTACGCGAAGGGGATATCGCCAATATGGGCTT
TGCCGAGGCGGTCGACTGCCCAGTGATCATTATTGCCGACATCGATAAAGGCGGGGTGTTTGCCCATTTGGTGGGTACCT
TAGCGCTATTGTCGCCCTCCGAGCAAGCTAGGGTAAAAGGCTTTGTTATCAATCGCTTTCGAGGCGATATCAGCCTATTG
CAGTCTGGTCTCGATTGGCTAGAGGCCCATACGCAAAAGCCTGTGTTGGGCGTGTTGCCCTATTTGCATGATTTGCATTT
AGACGCCGAGGATGCACTTATCGAGTCGCCCACTAAGCAGGCACAAAGCCGTTTTAAGGTGCGGGTATTAGTGTATCCCC
GCACCAGTAATCATACGGATTTTGACCCGCTTAGACTGCACCCCGATATCGATTTTGATTATGTCTCGCTCCAGACTCAG
GCCTCTGCCCAGACGCAGGAGATTGTGGCCGATCTGCTGATTTTACCCGGCAGTAAGAATGTCCGAGCAGACTTGGCTTT
TTTGCGTGAGCAGGGCTGGGATAAACAAATTGCCAAGCATCTACGCTACGGCGGTAAGCTGCTGGGGATTTGCGGCGGCT
ATCAAATGTTGGGTGAAAGCATTGCCGATCCCATGGGGATTGAAGACACGCTCGGCGTGAGCCAAGGGCTCGGATATTTA
CCCATTACCACCGAATTTAAGGCTGAAAAACAGCTACGTAGGGTCACTGGCCATTTAGCGCTGCAAGGGCAACAAGTAGA
GGTGAAGGGCTATGAGATCCACTGCGGTGAGTCCCAATATCTCACGTCCAGTGATGCCGCCAAGAGTGCGCCATTACGCT
TGAAAAGTGAGAGTGCTTGCGATCAACAGGCAGAAAAGCCTTTTGCCGATGGCTGTTTAAGTGACGACGGGCAAGTGCTC
GGCAGCTATTTACATGGGCTATTCGACAGCCCAGAGGCCTGCCAGCTCGTCCTTCACTGGGCTGGGTTGGAAAATGCTCA
AGCGATTGATATCAATGATATTCGCGAACAACAGTTGGATCGCTTGGCCGACGTATTAGCCGAGCACCTCGATATGCAAA
AGCTCGCATCCATTATAAATGCATAA

Upstream 100 bases:

>100_bases
TAAATCGAGTTAAGACAAAGTATTGTCGACGCATTCTTAGTAAGGGAGCGAACCGATTCCCCTTTAGTTTATTTTCCTCT
GTTGAGTGATGTTCTTGCCC

Downstream 100 bases:

>100_bases
GTGTTAGCAGTAGGAAGAACAGAAAATCATGACAGTAGAGAACGAAAAACAGCTCGACAACGAGCAAGCGCAAATCAAGG
CCGAACGCCATAAGGCGCGT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MNSNNASNPTSALSQNLFQGVVSQSEAQHSGAKVLMVQGTTSDAGKSTLVAGICRLLAREGVKVAPFKPQNMALNSAVTI
DGGEIGRAQALQAAACFLAPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGEKSKDYKAMALNAVLESFARLGQQ
YQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSPSEQARVKGFVINRFRGDISLL
QSGLDWLEAHTQKPVLGVLPYLHDLHLDAEDALIESPTKQAQSRFKVRVLVYPRTSNHTDFDPLRLHPDIDFDYVSLQTQ
ASAQTQEIVADLLILPGSKNVRADLAFLREQGWDKQIAKHLRYGGKLLGICGGYQMLGESIADPMGIEDTLGVSQGLGYL
PITTEFKAEKQLRRVTGHLALQGQQVEVKGYEIHCGESQYLTSSDAAKSAPLRLKSESACDQQAEKPFADGCLSDDGQVL
GSYLHGLFDSPEACQLVLHWAGLENAQAIDINDIREQQLDRLADVLAEHLDMQKLASIINA

Sequences:

>Translated_541_residues
MNSNNASNPTSALSQNLFQGVVSQSEAQHSGAKVLMVQGTTSDAGKSTLVAGICRLLAREGVKVAPFKPQNMALNSAVTI
DGGEIGRAQALQAAACFLAPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGEKSKDYKAMALNAVLESFARLGQQ
YQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSPSEQARVKGFVINRFRGDISLL
QSGLDWLEAHTQKPVLGVLPYLHDLHLDAEDALIESPTKQAQSRFKVRVLVYPRTSNHTDFDPLRLHPDIDFDYVSLQTQ
ASAQTQEIVADLLILPGSKNVRADLAFLREQGWDKQIAKHLRYGGKLLGICGGYQMLGESIADPMGIEDTLGVSQGLGYL
PITTEFKAEKQLRRVTGHLALQGQQVEVKGYEIHCGESQYLTSSDAAKSAPLRLKSESACDQQAEKPFADGCLSDDGQVL
GSYLHGLFDSPEACQLVLHWAGLENAQAIDINDIREQQLDRLADVLAEHLDMQKLASIINA
>Mature_541_residues
MNSNNASNPTSALSQNLFQGVVSQSEAQHSGAKVLMVQGTTSDAGKSTLVAGICRLLAREGVKVAPFKPQNMALNSAVTI
DGGEIGRAQALQAAACFLAPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGEKSKDYKAMALNAVLESFARLGQQ
YQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSPSEQARVKGFVINRFRGDISLL
QSGLDWLEAHTQKPVLGVLPYLHDLHLDAEDALIESPTKQAQSRFKVRVLVYPRTSNHTDFDPLRLHPDIDFDYVSLQTQ
ASAQTQEIVADLLILPGSKNVRADLAFLREQGWDKQIAKHLRYGGKLLGICGGYQMLGESIADPMGIEDTLGVSQGLGYL
PITTEFKAEKQLRRVTGHLALQGQQVEVKGYEIHCGESQYLTSSDAAKSAPLRLKSESACDQQAEKPFADGCLSDDGQVL
GSYLHGLFDSPEACQLVLHWAGLENAQAIDINDIREQQLDRLADVLAEHLDMQKLASIINA

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 58289; Mature: 58289

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNNASNPTSALSQNLFQGVVSQSEAQHSGAKVLMVQGTTSDAGKSTLVAGICRLLARE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHC
GVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAAACFLAPHTDFNPILLKPSSDTGAQII
CCEECCCCCCCCEECCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEE
VHGKALTTLEASAFFGEKSKDYKAMALNAVLESFARLGQQYQTIVVEGAGSPAEINLREG
EECCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCC
DIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSPSEQARVKGFVINRFRGDISLL
CCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHEEEEHHHHHHHHH
QSGLDWLEAHTQKPVLGVLPYLHDLHLDAEDALIESPTKQAQSRFKVRVLVYPRTSNHTD
HHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCEEEEEEEEECCCCCCC
FDPLRLHPDIDFDYVSLQTQASAQTQEIVADLLILPGSKNVRADLAFLREQGWDKQIAKH
CCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHH
LRYGGKLLGICGGYQMLGESIADPMGIEDTLGVSQGLGYLPITTEFKAEKQLRRVTGHLA
HHCCCCEEEECCCHHHHHHHHCCCCCCHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHE
LQGQQVEVKGYEIHCGESQYLTSSDAAKSAPLRLKSESACDQQAEKPFADGCLSDDGQVL
ECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCHHHH
GSYLHGLFDSPEACQLVLHWAGLENAQAIDINDIREQQLDRLADVLAEHLDMQKLASIIN
HHHHHHHCCCCHHHHHHHEECCCCCCCEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
A
C
>Mature Secondary Structure
MNSNNASNPTSALSQNLFQGVVSQSEAQHSGAKVLMVQGTTSDAGKSTLVAGICRLLARE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHC
GVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAAACFLAPHTDFNPILLKPSSDTGAQII
CCEECCCCCCCCEECCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEE
VHGKALTTLEASAFFGEKSKDYKAMALNAVLESFARLGQQYQTIVVEGAGSPAEINLREG
EECCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCC
DIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSPSEQARVKGFVINRFRGDISLL
CCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHEEEEHHHHHHHHH
QSGLDWLEAHTQKPVLGVLPYLHDLHLDAEDALIESPTKQAQSRFKVRVLVYPRTSNHTD
HHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCEEEEEEEEECCCCCCC
FDPLRLHPDIDFDYVSLQTQASAQTQEIVADLLILPGSKNVRADLAFLREQGWDKQIAKH
CCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHH
LRYGGKLLGICGGYQMLGESIADPMGIEDTLGVSQGLGYLPITTEFKAEKQLRRVTGHLA
HHCCCCEEEECCCHHHHHHHHCCCCCCHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHE
LQGQQVEVKGYEIHCGESQYLTSSDAAKSAPLRLKSESACDQQAEKPFADGCLSDDGQVL
ECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCHHHH
GSYLHGLFDSPEACQLVLHWAGLENAQAIDINDIREQQLDRLADVLAEHLDMQKLASIIN
HHHHHHHCCCCHHHHHHHEECCCCCCCEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]