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The map label for this gene is btuF [H]

Identifier: 117921710

GI number: 117921710

Start: 3911804

End: 3912628

Strand: Reverse

Name: btuF [H]

Synonym: Shewana3_3272

Alternate gene names: 117921710

Gene position: 3912628-3911804 (Counterclockwise)

Preceding gene: 117921711

Following gene: 117921709

Centisome position: 78.69

GC content: 49.94

Gene sequence:

>825_bases
ATGTTAATGCGACGCTTTATTGCCCTCGGCCTGAGCTTTGCGCTGTTACCCGCAGTGGCGATGGCCGAGCCTGCAAAACG
TATTATTGCGCTTTCGCCCCATGCGGTTGAAATGCTCTACGCCATCGGCGCAGGGGAGTCGATTGTGGCCGCGACAGATT
ATGCGGATTATCCCGAAGCGGCAAAAAAAATCCCCAGTATCGGTGGATATTATGGGATCCAGATTGAGCGGGTATTAGAG
CTTAATCCCGATCTGATTGTGGTGTGGGACACGGGCAATAAAGCCGAGGATATCAATCAACTCAAAAACTTAGGTTTTAA
ACTCTATAGCAGCTCCCCAAAGACGCTTGAGGATGTCGCCAAAGAAATTGAGGAGTTAGGTGAGCTGACAGGGCACACAG
AGCAGGCGAGTCGAGTGGCGGCGGATTATCGCAGTGAGCTGCTGCGATTACGTAGCGAGAATGCGGCTAAATCCGAGCCA
AAGGTGTTTTATCAGCTATGGTCTACGCCGTTAATGACGGTCGCGAAAAACAGTTGGATCCAGCAGATTATCGGTGTTTG
CCATGGCAAAAACGTTTTCTACGATGCGGCGAGTGATTACCCACAGGTGAGTTTAGAGAACGTATTACTGACCTTGCCCG
AGGTGATTTTACAGAGCGAAGAGGAGGGTAATGTCAAAGGGGTTGATTGGAGCCAATGGGCCGAAATCCCCGCGGTTAAA
AACCAGCATATTTATCAATTAAATGCCGACTTATTGCACCGCGCGACGCCCAGAGCGTTACTCGGAGTAAAAGCCGTATG
TGATGCCTTGGATAAAGCACGTTAA

Upstream 100 bases:

>100_bases
CTGGGATCGAGACCCTAAATATCGGCCTAAGAATCTGCAGACCTACCTTGAGCAGTACGGCTGGCCAGAAGAATTCATCG
ATTAAATCATAAGGAATATC

Downstream 100 bases:

>100_bases
CTAAAGCGTGTTACTAAAGTGCGTTAATAGTCTATTGCAGCAGGGAATGCGCGTAAGAGGAGGGGATAAAGATGTCGACA
CCAATCAATGTTAGTGTAGA

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MLMRRFIALGLSFALLPAVAMAEPAKRIIALSPHAVEMLYAIGAGESIVAATDYADYPEAAKKIPSIGGYYGIQIERVLE
LNPDLIVVWDTGNKAEDINQLKNLGFKLYSSSPKTLEDVAKEIEELGELTGHTEQASRVAADYRSELLRLRSENAAKSEP
KVFYQLWSTPLMTVAKNSWIQQIIGVCHGKNVFYDAASDYPQVSLENVLLTLPEVILQSEEEGNVKGVDWSQWAEIPAVK
NQHIYQLNADLLHRATPRALLGVKAVCDALDKAR

Sequences:

>Translated_274_residues
MLMRRFIALGLSFALLPAVAMAEPAKRIIALSPHAVEMLYAIGAGESIVAATDYADYPEAAKKIPSIGGYYGIQIERVLE
LNPDLIVVWDTGNKAEDINQLKNLGFKLYSSSPKTLEDVAKEIEELGELTGHTEQASRVAADYRSELLRLRSENAAKSEP
KVFYQLWSTPLMTVAKNSWIQQIIGVCHGKNVFYDAASDYPQVSLENVLLTLPEVILQSEEEGNVKGVDWSQWAEIPAVK
NQHIYQLNADLLHRATPRALLGVKAVCDALDKAR
>Mature_274_residues
MLMRRFIALGLSFALLPAVAMAEPAKRIIALSPHAVEMLYAIGAGESIVAATDYADYPEAAKKIPSIGGYYGIQIERVLE
LNPDLIVVWDTGNKAEDINQLKNLGFKLYSSSPKTLEDVAKEIEELGELTGHTEQASRVAADYRSELLRLRSENAAKSEP
KVFYQLWSTPLMTVAKNSWIQQIIGVCHGKNVFYDAASDYPQVSLENVLLTLPEVILQSEEEGNVKGVDWSQWAEIPAVK
NQHIYQLNADLLHRATPRALLGVKAVCDALDKAR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=273, Percent_Identity=30.7692307692308, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30293; Mature: 30293

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMRRFIALGLSFALLPAVAMAEPAKRIIALSPHAVEMLYAIGAGESIVAATDYADYPEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCCEEEECCCCCCCHH
AKKIPSIGGYYGIQIERVLELNPDLIVVWDTGNKAEDINQLKNLGFKLYSSSPKTLEDVA
HHHCCCCCCEECEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCEEEECCCCHHHHHHH
KEIEELGELTGHTEQASRVAADYRSELLRLRSENAAKSEPKVFYQLWSTPLMTVAKNSWI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHH
QQIIGVCHGKNVFYDAASDYPQVSLENVLLTLPEVILQSEEEGNVKGVDWSQWAEIPAVK
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCC
NQHIYQLNADLLHRATPRALLGVKAVCDALDKAR
CCEEEEECHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLMRRFIALGLSFALLPAVAMAEPAKRIIALSPHAVEMLYAIGAGESIVAATDYADYPEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCCEEEECCCCCCCHH
AKKIPSIGGYYGIQIERVLELNPDLIVVWDTGNKAEDINQLKNLGFKLYSSSPKTLEDVA
HHHCCCCCCEECEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCEEEECCCCHHHHHHH
KEIEELGELTGHTEQASRVAADYRSELLRLRSENAAKSEPKVFYQLWSTPLMTVAKNSWI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHH
QQIIGVCHGKNVFYDAASDYPQVSLENVLLTLPEVILQSEEEGNVKGVDWSQWAEIPAVK
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCC
NQHIYQLNADLLHRATPRALLGVKAVCDALDKAR
CCEEEEECHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA