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The map label for this gene is hyfR [H]

Identifier: 117921665

GI number: 117921665

Start: 3869605

End: 3871071

Strand: Direct

Name: hyfR [H]

Synonym: Shewana3_3227

Alternate gene names: 117921665

Gene position: 3869605-3871071 (Clockwise)

Preceding gene: 117921664

Following gene: 117921666

Centisome position: 77.82

GC content: 49.63

Gene sequence:

>1467_bases
ATGACATCAATAGAGCCCACCGCAATGCCCTTAGATAAACATTTTCTGCCCGAAAATCAGTTACTGCTTAATGCCGTGGG
TGAAGGTATTTATGGGTTTGATTTAAAGGGTAATGCTGTCTTTATCAATCCCGCCGCCGAGCGCATGACGGGCTGGAAAA
ATGAAGAGTTACTCGGCAAAAATATCCATAATTGCCATCATCATAGCCACGCGGATGGTAGTCATTATCCGCAGGAAGAT
TGCCCCATCTACAATACGTTAAAAGATGGGATAGCAAGGGAAATTACCCACGATGTATTCTGGCGCAAAGATGGTAGCAG
CTTTCCAGTGTATTACAGCTCTACGCCCGTCTATCGGGATAACAAGCTTATCGGAGTAGTCGCGATATTTCGCGATATTA
GTATTCAGAAACAAACCGAGCAGTCGCTACGCCAAGCTTTGGCGCAGGTTCAGGCTCTGTCGGAGCAACTTGCCAGCGAG
AATCACTATCTCCAGGCCGAGTTAGCCGATAAGACCGGCGATGTGGATATTTCGGGCAGTAGCGCGGTGATCCGCCATAT
GATCCAGCAATTGCATATGGTGGCTAATACCGATAGTACTGTACTGATCTGTGGCGAGAATGGCACAGGCAAAGAACTGG
TTGCCCGTAATTTACATCAACTGAGTCGCCGTAAAGATAAACCCATGATCAGCGTTAACTGCGCGGCGTTTTCAGCCTCA
CTGCTAGAGAGTGAGTTGTTTGGCCATGAGAAAGGCGCCTTTACCGGTGCAACTCAGCAACGCAAGGGCCGCTTCGAGCT
CGCCCATCAAGGCACACTGTTTCTCGATGAGGTGGCCGAATTAAGTTTAGAGGCGCAATCTAAGTTACTGCGGGTGATCC
AAGAGCAATCCTTTGAACGACTCGGCGGCAGCGAAACCATCAAGGTCGATATTCGCTTAGTCGCCGCCAGCCACCATGAT
TTACTCAAGCGCGTTGAACAGGGCTTATTCAGGATGGATCTCTATTACCGCTTGAATGTCTTCCCAATTCAAGTGCCGCC
GCTGAGGGCAAGGCTTGAAGATATGCCCGAGCTTGTCAGCCACATTTTGCATTCCCTAAACCGCAAACTCGGTAAGAAAC
TGCGCGGCGTAAGCCAAAAAGGTCTGCAAAAACTGATGGCCTACAGCTGGCCTGGCAATGTGCGCGAATTACAAAATATC
CTCGAACGGGAGGCCATTTTATCCCAAACGGATATTCTACAAATCCATGCCATGCCTACGCATCACCTACAGACTCAAAC
CGCAGCTTCGCCATTACAAACCTTGGCCGAGGCGGAAGCGCAACATATAGAACAAACATTAAAGCAGCTCAACTGGCGTA
TTTCAGGCCCCAATGGGGCGGCCAATGTGCTCGGCGTTCCGCCAAGTACACTCAGATCGCGGATGAAGAAACTCGGGATT
ACACGCCAAACCCCTGCAGAAGAATAG

Upstream 100 bases:

>100_bases
AAGCATTCACTTCAAGCATCACAGAGCAACTCACGATATATCGCGACACGATTCAAAGGGTTATACTTAGGGCACTCATT
CGTATTAAGGAATCAGCTGA

Downstream 100 bases:

>100_bases
CCTTTAAAAACGCGATATATCGCCAATTAGCGATATATCGCGACATGCGTTAAATCACCATTTAATTTAACCATTAAATA
TCAATACGTTAAAACAAAAA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTSIEPTAMPLDKHFLPENQLLLNAVGEGIYGFDLKGNAVFINPAAERMTGWKNEELLGKNIHNCHHHSHADGSHYPQED
CPIYNTLKDGIAREITHDVFWRKDGSSFPVYYSSTPVYRDNKLIGVVAIFRDISIQKQTEQSLRQALAQVQALSEQLASE
NHYLQAELADKTGDVDISGSSAVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFSAS
LLESELFGHEKGAFTGATQQRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFERLGGSETIKVDIRLVAASHHD
LLKRVEQGLFRMDLYYRLNVFPIQVPPLRARLEDMPELVSHILHSLNRKLGKKLRGVSQKGLQKLMAYSWPGNVRELQNI
LEREAILSQTDILQIHAMPTHHLQTQTAASPLQTLAEAEAQHIEQTLKQLNWRISGPNGAANVLGVPPSTLRSRMKKLGI
TRQTPAEE

Sequences:

>Translated_488_residues
MTSIEPTAMPLDKHFLPENQLLLNAVGEGIYGFDLKGNAVFINPAAERMTGWKNEELLGKNIHNCHHHSHADGSHYPQED
CPIYNTLKDGIAREITHDVFWRKDGSSFPVYYSSTPVYRDNKLIGVVAIFRDISIQKQTEQSLRQALAQVQALSEQLASE
NHYLQAELADKTGDVDISGSSAVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFSAS
LLESELFGHEKGAFTGATQQRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFERLGGSETIKVDIRLVAASHHD
LLKRVEQGLFRMDLYYRLNVFPIQVPPLRARLEDMPELVSHILHSLNRKLGKKLRGVSQKGLQKLMAYSWPGNVRELQNI
LEREAILSQTDILQIHAMPTHHLQTQTAASPLQTLAEAEAQHIEQTLKQLNWRISGPNGAANVLGVPPSTLRSRMKKLGI
TRQTPAEE
>Mature_487_residues
TSIEPTAMPLDKHFLPENQLLLNAVGEGIYGFDLKGNAVFINPAAERMTGWKNEELLGKNIHNCHHHSHADGSHYPQEDC
PIYNTLKDGIAREITHDVFWRKDGSSFPVYYSSTPVYRDNKLIGVVAIFRDISIQKQTEQSLRQALAQVQALSEQLASEN
HYLQAELADKTGDVDISGSSAVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFSASL
LESELFGHEKGAFTGATQQRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFERLGGSETIKVDIRLVAASHHDL
LKRVEQGLFRMDLYYRLNVFPIQVPPLRARLEDMPELVSHILHSLNRKLGKKLRGVSQKGLQKLMAYSWPGNVRELQNIL
EREAILSQTDILQIHAMPTHHLQTQTAASPLQTLAEAEAQHIEQTLKQLNWRISGPNGAANVLGVPPSTLRSRMKKLGIT
RQTPAEE

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=48.6880466472303, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=48.3870967741936, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1790437, Length=334, Percent_Identity=41.9161676646707, Blast_Score=257, Evalue=9e-70,
Organism=Escherichia coli, GI1788550, Length=296, Percent_Identity=43.2432432432432, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1788905, Length=274, Percent_Identity=41.2408759124088, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1790299, Length=330, Percent_Identity=39.0909090909091, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=38.801261829653, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=40.4388714733542, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1786524, Length=321, Percent_Identity=38.3177570093458, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=45.8515283842795, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=31.8611987381703, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=33.993399339934, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789828, Length=274, Percent_Identity=31.3868613138686, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54644; Mature: 54513

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIEPTAMPLDKHFLPENQLLLNAVGEGIYGFDLKGNAVFINPAAERMTGWKNEELLGK
CCCCCCCCCCCHHHCCCCCHHHHHHHCCCEEEEECCCCEEEECCHHHHHCCCCCHHHHHC
NIHNCHHHSHADGSHYPQEDCPIYNTLKDGIAREITHDVFWRKDGSSFPVYYSSTPVYRD
CHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
NKLIGVVAIFRDISIQKQTEQSLRQALAQVQALSEQLASENHYLQAELADKTGDVDISGS
CCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCC
SAVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFSAS
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
LLESELFGHEKGAFTGATQQRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFER
HHHHHHHCCCCCCCCCCCHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGSETIKVDIRLVAASHHDLLKRVEQGLFRMDLYYRLNVFPIQVPPLRARLEDMPELVS
CCCCCEEEEEEEEEECCHHHHHHHHHHHHHHEEHEEEEEEEEEECCHHHHHHHHHHHHHH
HILHSLNRKLGKKLRGVSQKGLQKLMAYSWPGNVRELQNILEREAILSQTDILQIHAMPT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
HHLQTQTAASPLQTLAEAEAQHIEQTLKQLNWRISGPNGAANVLGVPPSTLRSRMKKLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEECCCHHHHHHHHHHCCC
TRQTPAEE
CCCCCCCC
>Mature Secondary Structure 
TSIEPTAMPLDKHFLPENQLLLNAVGEGIYGFDLKGNAVFINPAAERMTGWKNEELLGK
CCCCCCCCCCHHHCCCCCHHHHHHHCCCEEEEECCCCEEEECCHHHHHCCCCCHHHHHC
NIHNCHHHSHADGSHYPQEDCPIYNTLKDGIAREITHDVFWRKDGSSFPVYYSSTPVYRD
CHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
NKLIGVVAIFRDISIQKQTEQSLRQALAQVQALSEQLASENHYLQAELADKTGDVDISGS
CCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCC
SAVIRHMIQQLHMVANTDSTVLICGENGTGKELVARNLHQLSRRKDKPMISVNCAAFSAS
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
LLESELFGHEKGAFTGATQQRKGRFELAHQGTLFLDEVAELSLEAQSKLLRVIQEQSFER
HHHHHHHCCCCCCCCCCCHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGSETIKVDIRLVAASHHDLLKRVEQGLFRMDLYYRLNVFPIQVPPLRARLEDMPELVS
CCCCCEEEEEEEEEECCHHHHHHHHHHHHHHEEHEEEEEEEEEECCHHHHHHHHHHHHHH
HILHSLNRKLGKKLRGVSQKGLQKLMAYSWPGNVRELQNILEREAILSQTDILQIHAMPT
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
HHLQTQTAASPLQTLAEAEAQHIEQTLKQLNWRISGPNGAANVLGVPPSTLRSRMKKLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEECCCHHHHHHHHHHCCC
TRQTPAEE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]