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The map label for this gene is dhfrIII [H]

Identifier: 117921652

GI number: 117921652

Start: 3858838

End: 3859320

Strand: Reverse

Name: dhfrIII [H]

Synonym: Shewana3_3214

Alternate gene names: 117921652

Gene position: 3859320-3858838 (Counterclockwise)

Preceding gene: 117921653

Following gene: 117921651

Centisome position: 77.62

GC content: 46.17

Gene sequence:

>483_bases
ATGCGCATTGCTATGATAGCCGCGATGGCAAACAATCGAGTCATTGGAAAAGACAACAAAATGCCATGGCATCTGCCCGA
AGATTTACGCCACTTTAAAGCCATGACCTTAGGCAAACCTGTGGTGATGGGGCGTAAAACCTTCGAATCCATTGGTAGAC
CCCTTCCAGGACGCCACAACATTGTGATTTCCCGCCAAGCGGATTTGCAGATAGAAGGCGTGACCTGCGTGACCTCTTTT
GAAGATGCTAAGCTCGCGGCTGGCGATTGTGAAGAGTTAGTCGTTATTGGTGGGGGGCAATTGTATCAACAATTATTACC
TCAAGCTGACAGACTCTACCTGACGCAAATTAACTTAGAGGTTGAAGGCGATACCTTTTTCCCCGCATGGGACAGCGATA
AATGGCAAGAAACCGAATCGCTCACTAGTATAAATGCTGATGGGCTGGAATATCGCTTTATCAATTTGGCAAAAAAATGT
TAA

Upstream 100 bases:

>100_bases
TTTATTTTAGGCGCGCTGTCGGTGGGCCTTGCTTTACCTAGTCTGCTGTATTTTAGAACCCGCCCTGTTATTCAATAAAA
ACAATACCAAAGGAGTGCCC

Downstream 100 bases:

>100_bases
ATTACGTTGTTCTGTTGTGTACCCTGTGCGTATGGATTGTCAATATGTCTAAGGTATTAGATCCGATAGGCCTGACTTAG
TTGAGATAGCGGCATTTTAC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRIAMIAAMANNRVIGKDNKMPWHLPEDLRHFKAMTLGKPVVMGRKTFESIGRPLPGRHNIVISRQADLQIEGVTCVTSF
EDAKLAAGDCEELVVIGGGQLYQQLLPQADRLYLTQINLEVEGDTFFPAWDSDKWQETESLTSINADGLEYRFINLAKKC

Sequences:

>Translated_160_residues
MRIAMIAAMANNRVIGKDNKMPWHLPEDLRHFKAMTLGKPVVMGRKTFESIGRPLPGRHNIVISRQADLQIEGVTCVTSF
EDAKLAAGDCEELVVIGGGQLYQQLLPQADRLYLTQINLEVEGDTFFPAWDSDKWQETESLTSINADGLEYRFINLAKKC
>Mature_160_residues
MRIAMIAAMANNRVIGKDNKMPWHLPEDLRHFKAMTLGKPVVMGRKTFESIGRPLPGRHNIVISRQADLQIEGVTCVTSF
EDAKLAAGDCEELVVIGGGQLYQQLLPQADRLYLTQINLEVEGDTFFPAWDSDKWQETESLTSINADGLEYRFINLAKKC

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=173, Percent_Identity=31.7919075144509, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1786233, Length=138, Percent_Identity=55.7971014492754, Blast_Score=158, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17505899, Length=178, Percent_Identity=31.4606741573034, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI116008026, Length=147, Percent_Identity=34.0136054421769, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24647458, Length=147, Percent_Identity=34.0136054421769, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17979; Mature: 17979

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAMIAAMANNRVIGKDNKMPWHLPEDLRHFKAMTLGKPVVMGRKTFESIGRPLPGRHN
CEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRQADLQIEGVTCVTSFEDAKLAAGDCEELVVIGGGQLYQQLLPQADRLYLTQINLE
EEEEECCCEEECCEEEEECCCCCEECCCCCCEEEEECCHHHHHHHCCCCCEEEEEEEEEE
VEGDTFFPAWDSDKWQETESLTSINADGLEYRFINLAKKC
ECCCEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEEHHCC
>Mature Secondary Structure
MRIAMIAAMANNRVIGKDNKMPWHLPEDLRHFKAMTLGKPVVMGRKTFESIGRPLPGRHN
CEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCC
IVISRQADLQIEGVTCVTSFEDAKLAAGDCEELVVIGGGQLYQQLLPQADRLYLTQINLE
EEEEECCCEEECCEEEEECCCCCEECCCCCCEEEEECCHHHHHHHCCCCCEEEEEEEEEE
VEGDTFFPAWDSDKWQETESLTSINADGLEYRFINLAKKC
ECCCEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]