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The map label for this gene is rstB [H]

Identifier: 117921623

GI number: 117921623

Start: 3823521

End: 3824789

Strand: Direct

Name: rstB [H]

Synonym: Shewana3_3185

Alternate gene names: 117921623

Gene position: 3823521-3824789 (Clockwise)

Preceding gene: 117921622

Following gene: 117921625

Centisome position: 76.9

GC content: 49.88

Gene sequence:

>1269_bases
GTGAAACGTCTCTTTATCAGCTTATATTTACTGCTGAGCCTCAGTTTTCTAGGCATAGGCTGGACACTGGACAGCCTGTG
GCAGAAAAATGTGGACGAAAATAATGCGTTAGACGCTCCTCTGGTTGCGTTAGCCCAGTTGCTAACCCAACTGCCAGAGG
AGAATCGTAACGACTATCTTGAGGCGCTGCCCAGCCATGATCAGTTGCCCCTTAATCTGCTCAGCCCAGATCAAATTGCG
CTAGCCAAAGACCAACAGCTCACGCCCAATCATGTGGTTACCACCACAACCAGCGAGGGCGAACAATTCCAGTTTATTCA
GGTTGGCGAGCAAGTGTTAATGGCAGGTCCCATCGAAATAGACCCAAGGGCCTCACTGCGGAATCTGTTTACGCTGTTTT
TCTATCTCTCGTTGGCATTGGTTGCGCTGATTTGGGTCGGCCCGTTGTCGCGTGATTTGAGTATTCTACGTAAGGCCACC
AAGGATTTTGGCGAGGCCAAATGGGATACACGCATTAGCCTCTCAAACCGCTCGCAGGTTAAACCCTTAGCGAACACCTT
TAATGAAATGGCGCGCCATATCAGTGCGTTAATCGAAAACCAAAAACATTTATCCAATGCGGTTTCCCACGAGATCCGCA
CCCCACTCGCACGGCTGAAATTCGCCCTCGCCCTAATGCCGATGTACTGCCAGCCCGATTCTGACGAGCAAAAGCGTCAG
GATTTTCTCAATGAGATGCAGCTCGACATTAAGGAAATGGAAAACCTGCTGCAGGAACTGCTGACTTACGCCAGTCTTGA
GGCTCAGCGCGAAGGCACGCCTTTAGACGAATACGATCTGCTTAGCTTAGTTAACCAGAGCATCAAACGCTTGAGCAGCT
TAAGTGATACCCCTATCCACTTACAGGCGGAGATGACAACTCTCCCCTTGGTCGGCGAACCCGCTCTGATTGAACGCGCA
CTACAAAACCTGCTGACCAACGCCCAGCGTTTTGCTCGCCAGCAAATATTGATTGAAGTCAGCCAAACCCCGCAAAGGGT
GACTTTAAGTGTCAGTAATGATGGGGAGCCTATTCCCGAGGTGGATTTACCTATGGTGTTCGAGCCCTTTTATCGCAGCC
AATCGGTGCAAAATGGTAACAAGGGTCATGGCTTAGGTCTGGCGATTATCAAACGAATTATGCAACGTCATCAGGGCGAA
GTAAGCGTCAGCAGCAATCCCACTCAAACGCGCTTTACCTTAAGCTGGCCAAAGAAACGGGAAATTTAA

Upstream 100 bases:

>100_bases
AAAAGCTCAACGACAATATCGAGACCCCATTCAGAATTAAAACCGTCTGGGGACAGGGTTATCTCTTCGCCCCCGAGGCT
TGGAACAACTAAGAGCTGCC

Downstream 100 bases:

>100_bases
GCCCTTAAGTATCGCTCTCAAAGTTAGATTATTCATCGAGGTCAAAATCCCCGAGGTCTAACTCAGCGGCGTCTTGCGGC
TCAAAATCGAGTTGTGGATT

Product: ATPase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKRLFISLYLLLSLSFLGIGWTLDSLWQKNVDENNALDAPLVALAQLLTQLPEENRNDYLEALPSHDQLPLNLLSPDQIA
LAKDQQLTPNHVVTTTTSEGEQFQFIQVGEQVLMAGPIEIDPRASLRNLFTLFFYLSLALVALIWVGPLSRDLSILRKAT
KDFGEAKWDTRISLSNRSQVKPLANTFNEMARHISALIENQKHLSNAVSHEIRTPLARLKFALALMPMYCQPDSDEQKRQ
DFLNEMQLDIKEMENLLQELLTYASLEAQREGTPLDEYDLLSLVNQSIKRLSSLSDTPIHLQAEMTTLPLVGEPALIERA
LQNLLTNAQRFARQQILIEVSQTPQRVTLSVSNDGEPIPEVDLPMVFEPFYRSQSVQNGNKGHGLGLAIIKRIMQRHQGE
VSVSSNPTQTRFTLSWPKKREI

Sequences:

>Translated_422_residues
MKRLFISLYLLLSLSFLGIGWTLDSLWQKNVDENNALDAPLVALAQLLTQLPEENRNDYLEALPSHDQLPLNLLSPDQIA
LAKDQQLTPNHVVTTTTSEGEQFQFIQVGEQVLMAGPIEIDPRASLRNLFTLFFYLSLALVALIWVGPLSRDLSILRKAT
KDFGEAKWDTRISLSNRSQVKPLANTFNEMARHISALIENQKHLSNAVSHEIRTPLARLKFALALMPMYCQPDSDEQKRQ
DFLNEMQLDIKEMENLLQELLTYASLEAQREGTPLDEYDLLSLVNQSIKRLSSLSDTPIHLQAEMTTLPLVGEPALIERA
LQNLLTNAQRFARQQILIEVSQTPQRVTLSVSNDGEPIPEVDLPMVFEPFYRSQSVQNGNKGHGLGLAIIKRIMQRHQGE
VSVSSNPTQTRFTLSWPKKREI
>Mature_422_residues
MKRLFISLYLLLSLSFLGIGWTLDSLWQKNVDENNALDAPLVALAQLLTQLPEENRNDYLEALPSHDQLPLNLLSPDQIA
LAKDQQLTPNHVVTTTTSEGEQFQFIQVGEQVLMAGPIEIDPRASLRNLFTLFFYLSLALVALIWVGPLSRDLSILRKAT
KDFGEAKWDTRISLSNRSQVKPLANTFNEMARHISALIENQKHLSNAVSHEIRTPLARLKFALALMPMYCQPDSDEQKRQ
DFLNEMQLDIKEMENLLQELLTYASLEAQREGTPLDEYDLLSLVNQSIKRLSSLSDTPIHLQAEMTTLPLVGEPALIERA
LQNLLTNAQRFARQQILIEVSQTPQRVTLSVSNDGEPIPEVDLPMVFEPFYRSQSVQNGNKGHGLGLAIIKRIMQRHQGE
VSVSSNPTQTRFTLSWPKKREI

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=438, Percent_Identity=27.6255707762557, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786783, Length=246, Percent_Identity=27.6422764227642, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790346, Length=236, Percent_Identity=26.6949152542373, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789808, Length=230, Percent_Identity=27.3913043478261, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87082128, Length=280, Percent_Identity=26.7857142857143, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790861, Length=200, Percent_Identity=32.5, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=28.7671232876712, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=27.3127753303965, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=278, Percent_Identity=22.6618705035971, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=24.609375, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=219, Percent_Identity=28.7671232876712, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789403, Length=244, Percent_Identity=25.4098360655738, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790551, Length=250, Percent_Identity=24.8, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=27.9279279279279, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47767; Mature: 47767

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFISLYLLLSLSFLGIGWTLDSLWQKNVDENNALDAPLVALAQLLTQLPEENRNDYL
CHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
EALPSHDQLPLNLLSPDQIALAKDQQLTPNHVVTTTTSEGEQFQFIQVGEQVLMAGPIEI
HHCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCC
DPRASLRNLFTLFFYLSLALVALIWVGPLSRDLSILRKATKDFGEAKWDTRISLSNRSQV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
KPLANTFNEMARHISALIENQKHLSNAVSHEIRTPLARLKFALALMPMYCQPDSDEQKRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DFLNEMQLDIKEMENLLQELLTYASLEAQREGTPLDEYDLLSLVNQSIKRLSSLSDTPIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
LQAEMTTLPLVGEPALIERALQNLLTNAQRFARQQILIEVSQTPQRVTLSVSNDGEPIPE
EEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCC
VDLPMVFEPFYRSQSVQNGNKGHGLGLAIIKRIMQRHQGEVSVSSNPTQTRFTLSWPKKR
CCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCC
EI
CC
>Mature Secondary Structure
MKRLFISLYLLLSLSFLGIGWTLDSLWQKNVDENNALDAPLVALAQLLTQLPEENRNDYL
CHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
EALPSHDQLPLNLLSPDQIALAKDQQLTPNHVVTTTTSEGEQFQFIQVGEQVLMAGPIEI
HHCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCC
DPRASLRNLFTLFFYLSLALVALIWVGPLSRDLSILRKATKDFGEAKWDTRISLSNRSQV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
KPLANTFNEMARHISALIENQKHLSNAVSHEIRTPLARLKFALALMPMYCQPDSDEQKRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DFLNEMQLDIKEMENLLQELLTYASLEAQREGTPLDEYDLLSLVNQSIKRLSSLSDTPIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
LQAEMTTLPLVGEPALIERALQNLLTNAQRFARQQILIEVSQTPQRVTLSVSNDGEPIPE
EEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCC
VDLPMVFEPFYRSQSVQNGNKGHGLGLAIIKRIMQRHQGEVSVSSNPTQTRFTLSWPKKR
CCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCC
EI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]