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The map label for this gene is rsuA [H]

Identifier: 117921613

GI number: 117921613

Start: 3816350

End: 3817075

Strand: Reverse

Name: rsuA [H]

Synonym: Shewana3_3175

Alternate gene names: 117921613

Gene position: 3817075-3816350 (Counterclockwise)

Preceding gene: 117921614

Following gene: 117921609

Centisome position: 76.77

GC content: 48.62

Gene sequence:

>726_bases
ATGCAATCAAAACGTGGTCGTCTCGACAGATATCTTGCCGTTAAGCTGCAAATTCACCGTAAAGCCGTGCGAGAACTCTT
GCTGACGGAGCGAGTATATGTGGATGGTATCCCCGCCAAAGCGCTCGATCTGCTGGTGGATGAATTTTCCCATATTGTAT
TTGATGGGCAAGTCTTACAGGCCAATAAGCCCGTTTATCTAATGCTGCACAAGCCTGTTGGTGTAGTGAGTGCCACCAAA
GACACAGAGCACCGCACAGTGATTGATTTACTCGACTGTGATGCTCGCCATGAGCTGCATATTGCTGGGCGGTTAGATTT
AAATTCTTCGGGTTTATTGCTGCTGACCAATGATAGCCGTTGGTCCGAGGCGCTCATGTCGCCCGATCAAAAGGTGGATA
AAGTGTATCGGGTGACTTTAGCGAATCCTTTGAGTGAGGAGTATATCGCGGCATTCGCGCAGGGCATGTATTTTGGCTTT
GAAGATATCACCACACAGCCAGCAAAGCTGACTATCCTCGACACTCATATTGCAGAAGTCACGTTAAGGGAAGGAAAGTA
CCATCAAATCAAGCGGATGTTTGGTCGTTTCCGTAATCCAGTGGTGGGATTGCATCGCCATTCTATCGGTGAGATTACCT
TGGATGATGGCTTAAAGCCGGGCGAGAGTCGTATGTTAACGGCGCAGGAGGTGGCTTCTGTCTATCTTGCGGACAAGTTA
GCCTAG

Upstream 100 bases:

>100_bases
TTGCCGATATCGCTACTGTAAGCCGTGAAGAGCATATCATCGACGGCGCCCGTCGCTGCGCCTATCGCATTGCACCTTTA
TCTTAGCGCTGGGTGGGGTT

Downstream 100 bases:

>100_bases
TTCAGCGTTTATGGAACAAGCATTAAGCCACCTTGCCTTACGGGAAGATGGCTTTGTGCTTGAGTTATTATCGATGATTG
ATAACGACGTTGCAGCCTTA

Product: pseudouridine synthase, Rsu

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MQSKRGRLDRYLAVKLQIHRKAVRELLLTERVYVDGIPAKALDLLVDEFSHIVFDGQVLQANKPVYLMLHKPVGVVSATK
DTEHRTVIDLLDCDARHELHIAGRLDLNSSGLLLLTNDSRWSEALMSPDQKVDKVYRVTLANPLSEEYIAAFAQGMYFGF
EDITTQPAKLTILDTHIAEVTLREGKYHQIKRMFGRFRNPVVGLHRHSIGEITLDDGLKPGESRMLTAQEVASVYLADKL
A

Sequences:

>Translated_241_residues
MQSKRGRLDRYLAVKLQIHRKAVRELLLTERVYVDGIPAKALDLLVDEFSHIVFDGQVLQANKPVYLMLHKPVGVVSATK
DTEHRTVIDLLDCDARHELHIAGRLDLNSSGLLLLTNDSRWSEALMSPDQKVDKVYRVTLANPLSEEYIAAFAQGMYFGF
EDITTQPAKLTILDTHIAEVTLREGKYHQIKRMFGRFRNPVVGLHRHSIGEITLDDGLKPGESRMLTAQEVASVYLADKL
A
>Mature_241_residues
MQSKRGRLDRYLAVKLQIHRKAVRELLLTERVYVDGIPAKALDLLVDEFSHIVFDGQVLQANKPVYLMLHKPVGVVSATK
DTEHRTVIDLLDCDARHELHIAGRLDLNSSGLLLLTNDSRWSEALMSPDQKVDKVYRVTLANPLSEEYIAAFAQGMYFGF
EDITTQPAKLTILDTHIAEVTLREGKYHQIKRMFGRFRNPVVGLHRHSIGEITLDDGLKPGESRMLTAQEVASVYLADKL
A

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=41.5584415584416, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=28.3261802575107, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787524, Length=230, Percent_Identity=29.5652173913043, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27215; Mature: 27215

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKRGRLDRYLAVKLQIHRKAVRELLLTERVYVDGIPAKALDLLVDEFSHIVFDGQVLQ
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHEECCEEEE
ANKPVYLMLHKPVGVVSATKDTEHRTVIDLLDCDARHELHIAGRLDLNSSGLLLLTNDSR
CCCCEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCEEEEECCCH
WSEALMSPDQKVDKVYRVTLANPLSEEYIAAFAQGMYFGFEDITTQPAKLTILDTHIAEV
HHHHHCCCHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEEEEEE
TLREGKYHQIKRMFGRFRNPVVGLHRHSIGEITLDDGLKPGESRMLTAQEVASVYLADKL
EECCCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCCCEEEHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MQSKRGRLDRYLAVKLQIHRKAVRELLLTERVYVDGIPAKALDLLVDEFSHIVFDGQVLQ
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHEECCEEEE
ANKPVYLMLHKPVGVVSATKDTEHRTVIDLLDCDARHELHIAGRLDLNSSGLLLLTNDSR
CCCCEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCEEEEECCCH
WSEALMSPDQKVDKVYRVTLANPLSEEYIAAFAQGMYFGFEDITTQPAKLTILDTHIAEV
HHHHHCCCHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEEEEEE
TLREGKYHQIKRMFGRFRNPVVGLHRHSIGEITLDDGLKPGESRMLTAQEVASVYLADKL
EECCCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCCCEEEHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]