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The map label for this gene is gshA [H]

Identifier: 117921598

GI number: 117921598

Start: 3788913

End: 3790562

Strand: Reverse

Name: gshA [H]

Synonym: Shewana3_3160

Alternate gene names: 117921598

Gene position: 3790562-3788913 (Counterclockwise)

Preceding gene: 117921599

Following gene: 117921597

Centisome position: 76.24

GC content: 48.3

Gene sequence:

>1650_bases
TTGAAAGATAAGCCTCAAAATAAAGGTCTGACTGTTAACCTTGTCGCGATAGCGAATCACCAATCAGAGAAGCGCAAATT
GAAGCCATTCAATGAATTAGTACAACATTTCTCGGACGCTCAAGGGCGTGCCGCACTCCTTGGCATGTTACGTGGAATTG
AGCGTGAAGCGCTGCGTATCGACGAGTCAGGCTACCTTGCGTTAGATGGTCATCCATTAGAGCTTGGCTCGGCATTAACG
CATTCGCGCATTACGACAGATTACAGTGAAGCACTGCTTGAATTTATCACCCCAGTAAACAATCAAGTTGGCCCCCTATT
GCAAGGACTCACCGAGACCCATGCCTTTAGCGTGCGTCATTTGCATGGTCAGCGCCTGTGGCCTGTGAGCATGCCATGTT
ACGTGAAGGATGAGCAGAATATTCCGGTCGCCCGTTATGGCAGTTCTAACACGGGTAAGATGAAAACCTTGTATCGCAAG
GGCTTAACCTACCGTTATGGCGCTCTGATGCAGATCATCTCTGGGGTGCATTTTAACTTCTCGGTATCGCAGGAGCTGTG
GCAAAGCCTCTATGAGTTGAGTGACAAAAGCGTGAGCTTTGAGGATTTTATCTCCGAATCTTACTTTGGCCTCATTCGCA
ATTACCGCCGTTTAGTCTGGGTGTTACCTTATCTGTTTGGGGCATCACCTGCGCTGTGTAGCTCGTTTATCAAGGGGCAG
AAGACCGATTTACGCTTTGAAAAATCGGGCCGTGGCACCTTGTATCTACCCTATGCGACTTCGCTGCGGATGAGCGATTT
AGGTTACACCAATAAAGAGCAAGAAGATCTTAATATCAGTTATAACTCCCTGCCCGAGTATCTGGCTGGGGTGAGAGCCG
CGATTAAGATGTCATCGGCCAACTTTGCCAATATCGGGGTGAAGGTGGATGGTGAATATCGCCAGCTCAATGCCAATGTG
CTGCAAATTGAAAACGAATTTTACTCGCCTATCCGTGCTAAACGCGTCACCAAAAGCGGTGAGAAACCCTCTGAAGCCCT
TGCCCGTGCGGGGGTTGAATATATTGAGGTGCGTGCATTAGATGTAAACCCCTTCAGCCCGATTGGGATTGAAGAGAACC
AAGTGCGTTTCCTCGACTTGTTTTTACTCTATTGCTTATTAACCGAGTCGCCTAAGTGCGATGTGGCAGAAGAAGCCCGT
TTAAGTGCTAACTTAAAATCCGTCGTGCTTGAAGGCCGTAAACCTGGGCTCGAGTTATTAACCGAGACTGGTACACGCTC
ACTGCAAACGTGGCTTTTGGATCTGTTTGAGCATTTGGATGCATTGGCCAAATTGCTCGATGGTGATAAACAGGATTACC
AAGCGGCCTTAGCCCATTGGCGTGAAGCCGTGAATGACCCACAAAAGACCTTGTCAGGGCTGGTCATTCAGCAATTAATC
ACTAACGGCCAAGACCATGGACAATGGGTGATGTCACTGGCGCAGCAATATCACCAATATTTTATGGATTACCCCTTATC
TGCCGAGGTTGTCAGCGATTATCAAGCTGAGGCGCAAGCATCGCTTAACAAGCAAGCGGACATCGAAGCGGCGCAAAGTG
CGGTATCCTTTGATGATTATCTGACGGACTATTTCGGGACTTCATCCTAA

Upstream 100 bases:

>100_bases
AGACGGTGACAGCATTAGCTGTCACCGTCTGCGTTTTATCGCAGAAGAATGTAATTAGGCTTGCTCCACGGACAGGTTTA
GATTTATTTTGAGGCGTATT

Downstream 100 bases:

>100_bases
TGAGTCACTATTTAACTGGGCACAAACTTACACTTGCCGCTAGTCTACTTGGGCTCACGGCATTGTTGTCTGGTTGTGTC
TCCTCGCCTGAAGATGACTT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKDKPQNKGLTVNLVAIANHQSEKRKLKPFNELVQHFSDAQGRAALLGMLRGIEREALRIDESGYLALDGHPLELGSALT
HSRITTDYSEALLEFITPVNNQVGPLLQGLTETHAFSVRHLHGQRLWPVSMPCYVKDEQNIPVARYGSSNTGKMKTLYRK
GLTYRYGALMQIISGVHFNFSVSQELWQSLYELSDKSVSFEDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKGQ
KTDLRFEKSGRGTLYLPYATSLRMSDLGYTNKEQEDLNISYNSLPEYLAGVRAAIKMSSANFANIGVKVDGEYRQLNANV
LQIENEFYSPIRAKRVTKSGEKPSEALARAGVEYIEVRALDVNPFSPIGIEENQVRFLDLFLLYCLLTESPKCDVAEEAR
LSANLKSVVLEGRKPGLELLTETGTRSLQTWLLDLFEHLDALAKLLDGDKQDYQAALAHWREAVNDPQKTLSGLVIQQLI
TNGQDHGQWVMSLAQQYHQYFMDYPLSAEVVSDYQAEAQASLNKQADIEAAQSAVSFDDYLTDYFGTSS

Sequences:

>Translated_549_residues
MKDKPQNKGLTVNLVAIANHQSEKRKLKPFNELVQHFSDAQGRAALLGMLRGIEREALRIDESGYLALDGHPLELGSALT
HSRITTDYSEALLEFITPVNNQVGPLLQGLTETHAFSVRHLHGQRLWPVSMPCYVKDEQNIPVARYGSSNTGKMKTLYRK
GLTYRYGALMQIISGVHFNFSVSQELWQSLYELSDKSVSFEDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKGQ
KTDLRFEKSGRGTLYLPYATSLRMSDLGYTNKEQEDLNISYNSLPEYLAGVRAAIKMSSANFANIGVKVDGEYRQLNANV
LQIENEFYSPIRAKRVTKSGEKPSEALARAGVEYIEVRALDVNPFSPIGIEENQVRFLDLFLLYCLLTESPKCDVAEEAR
LSANLKSVVLEGRKPGLELLTETGTRSLQTWLLDLFEHLDALAKLLDGDKQDYQAALAHWREAVNDPQKTLSGLVIQQLI
TNGQDHGQWVMSLAQQYHQYFMDYPLSAEVVSDYQAEAQASLNKQADIEAAQSAVSFDDYLTDYFGTSS
>Mature_549_residues
MKDKPQNKGLTVNLVAIANHQSEKRKLKPFNELVQHFSDAQGRAALLGMLRGIEREALRIDESGYLALDGHPLELGSALT
HSRITTDYSEALLEFITPVNNQVGPLLQGLTETHAFSVRHLHGQRLWPVSMPCYVKDEQNIPVARYGSSNTGKMKTLYRK
GLTYRYGALMQIISGVHFNFSVSQELWQSLYELSDKSVSFEDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKGQ
KTDLRFEKSGRGTLYLPYATSLRMSDLGYTNKEQEDLNISYNSLPEYLAGVRAAIKMSSANFANIGVKVDGEYRQLNANV
LQIENEFYSPIRAKRVTKSGEKPSEALARAGVEYIEVRALDVNPFSPIGIEENQVRFLDLFLLYCLLTESPKCDVAEEAR
LSANLKSVVLEGRKPGLELLTETGTRSLQTWLLDLFEHLDALAKLLDGDKQDYQAALAHWREAVNDPQKTLSGLVIQQLI
TNGQDHGQWVMSLAQQYHQYFMDYPLSAEVVSDYQAEAQASLNKQADIEAAQSAVSFDDYLTDYFGTSS

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=494, Percent_Identity=50, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 61740; Mature: 61740

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKPQNKGLTVNLVAIANHQSEKRKLKPFNELVQHFSDAQGRAALLGMLRGIEREALRI
CCCCCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHEE
DESGYLALDGHPLELGSALTHSRITTDYSEALLEFITPVNNQVGPLLQGLTETHAFSVRH
CCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LHGQRLWPVSMPCYVKDEQNIPVARYGSSNTGKMKTLYRKGLTYRYGALMQIISGVHFNF
HCCCEECCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCE
SVSQELWQSLYELSDKSVSFEDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKGQ
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
KTDLRFEKSGRGTLYLPYATSLRMSDLGYTNKEQEDLNISYNSLPEYLAGVRAAIKMSSA
CCCCEEECCCCCEEEECCCCCEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
NFANIGVKVDGEYRQLNANVLQIENEFYSPIRAKRVTKSGEKPSEALARAGVEYIEVRAL
CEEEEEEEECCCHHHHCCHHEEECHHHHCHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEE
DVNPFSPIGIEENQVRFLDLFLLYCLLTESPKCDVAEEARLSANLKSVVLEGRKPGLELL
ECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
TETGTRSLQTWLLDLFEHLDALAKLLDGDKQDYQAALAHWREAVNDPQKTLSGLVIQQLI
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TNGQDHGQWVMSLAQQYHQYFMDYPLSAEVVSDYQAEAQASLNKQADIEAAQSAVSFDDY
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LTDYFGTSS
HHHHCCCCC
>Mature Secondary Structure
MKDKPQNKGLTVNLVAIANHQSEKRKLKPFNELVQHFSDAQGRAALLGMLRGIEREALRI
CCCCCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHEE
DESGYLALDGHPLELGSALTHSRITTDYSEALLEFITPVNNQVGPLLQGLTETHAFSVRH
CCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LHGQRLWPVSMPCYVKDEQNIPVARYGSSNTGKMKTLYRKGLTYRYGALMQIISGVHFNF
HCCCEECCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCE
SVSQELWQSLYELSDKSVSFEDFISESYFGLIRNYRRLVWVLPYLFGASPALCSSFIKGQ
EHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
KTDLRFEKSGRGTLYLPYATSLRMSDLGYTNKEQEDLNISYNSLPEYLAGVRAAIKMSSA
CCCCEEECCCCCEEEECCCCCEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
NFANIGVKVDGEYRQLNANVLQIENEFYSPIRAKRVTKSGEKPSEALARAGVEYIEVRAL
CEEEEEEEECCCHHHHCCHHEEECHHHHCHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEE
DVNPFSPIGIEENQVRFLDLFLLYCLLTESPKCDVAEEARLSANLKSVVLEGRKPGLELL
ECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
TETGTRSLQTWLLDLFEHLDALAKLLDGDKQDYQAALAHWREAVNDPQKTLSGLVIQQLI
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TNGQDHGQWVMSLAQQYHQYFMDYPLSAEVVSDYQAEAQASLNKQADIEAAQSAVSFDDY
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LTDYFGTSS
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]