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The map label for this gene is puuB [H]

Identifier: 117921539

GI number: 117921539

Start: 3720060

End: 3721367

Strand: Direct

Name: puuB [H]

Synonym: Shewana3_3101

Alternate gene names: 117921539

Gene position: 3720060-3721367 (Clockwise)

Preceding gene: 117921528

Following gene: 117921544

Centisome position: 74.82

GC content: 50.69

Gene sequence:

>1308_bases
ATGACCACCAAACCTCCCGTTCATAGTGAACAATATCCCCAGTCTTATTATGTTGATACTGCAAAAGAAATTTACGCCCA
TCCCCAACTTGAGGGCAAGGTAAGCACCGACGTATGCATTATCGGCGGGGGGTTCAGCGGTATTAACACGGCTATCGAAC
TGCGGCAAAAGGGCCTAAACGTGGTGCTACTCGAGGCCAAGCGCATAGGCTGGGGCGCCTCAGGGCGTAATGGTGGCGAG
CTGATCCGCGGTATTGGCCATGATCCTGCGCAATTTATCAATGAGATAGGCCAAGAAGGTGTGAGCGCCATTGAACAGAT
GGGCTTTGAGGCGGTAGAGATTGTACGAAATCGGATACAGACACATCACATCGATTGCGACTTACAAATGGGCTATTGCG
ATTTGGCGGTTAAACCTCGGCATATGCTCGAACTAGAACAAGAGTTCGACCACCTTAAGCGCCTTGGATATCAGCATGAA
ATCAAGTTATTAACTCAGTCTCAGCTCGGAGAAGTTATCGGCTCAGACTTTTACCAAGGCGCCCTAGTCGACATGGGCAG
CGGTCATTTACATCCGCTGAATTTGGCCTTGGGCGAGGCTCGTGTTGCCCGAGACTTGGGCGCTCAATTATTTGAATACA
GCGCCGCAGAAAAGATTATTCCTAGGGATAAACCGCGAGTATTAACGGCAAAAGGCGAAGTGACTTGCCAATATGTCGTG
CTGGCGGGCAACGCTTATATAGGCCATAAACTGAATAGCTACATCGGCGGTAAAGTGCTGCCCGCAGGGAGCTACTTGCT
TGCAACCGAGCCACTGACAGAAGTGCAATGCCAAAGCATTATCCCGCAAAATATGGCCTTTGCAGATATGCGAATCGCCT
TAGATTACTTTCACTTATCGGCGGATAGACGCTTACTCTTCGGCGGACTTTGTACGTATTCAGGCAAAGATCCTAAGGAT
ATTGAAGCAGCGCTCAGGCCGAACCTTGAGAAGGTATTCCCACAGTTAAAAGGCGTACGCATAGATTACGAATGGGGCGG
CATGATAGGCATTGGCGCCAACCGTATGCCGCAAATCGGTCGCCTCCCCGATGCGAGCAATATTTTTTACGCCCAAGCCT
ATGCAGGCCACGGCGTAAATGCCACCCATATGGCGGCCAAACTGCTCGCCGAAGCCATTTGTCAGCAGGCGGGATATTTT
GATGTATTCGCCAAAATCAAGCATATGACCTTCCCCGGCGGCCCCACATTCCGCTCGCCTATGCTGGCTGCGGGTATGCT
GTACCACAGGTTTAAGGACTTATTCTGA

Upstream 100 bases:

>100_bases
GTGTTAAATAAAATGCACACTTTTTAGCAGTATCACAAAAGTGTGATGAGTATACTGGGACAAGCCCAATTTACAATAAA
AACAATTTTTTGGAGCTCAA

Downstream 100 bases:

>100_bases
GGTTTGCCCTGTCGTTGGATATGCGAGCAAGCTATCCAACGACACTAAATGGCGCGAGCCAATTTAATCCGCCTACGGCG
CCTTGACCACAGAATAAAAG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTTKPPVHSEQYPQSYYVDTAKEIYAHPQLEGKVSTDVCIIGGGFSGINTAIELRQKGLNVVLLEAKRIGWGASGRNGGE
LIRGIGHDPAQFINEIGQEGVSAIEQMGFEAVEIVRNRIQTHHIDCDLQMGYCDLAVKPRHMLELEQEFDHLKRLGYQHE
IKLLTQSQLGEVIGSDFYQGALVDMGSGHLHPLNLALGEARVARDLGAQLFEYSAAEKIIPRDKPRVLTAKGEVTCQYVV
LAGNAYIGHKLNSYIGGKVLPAGSYLLATEPLTEVQCQSIIPQNMAFADMRIALDYFHLSADRRLLFGGLCTYSGKDPKD
IEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIGRLPDASNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYF
DVFAKIKHMTFPGGPTFRSPMLAAGMLYHRFKDLF

Sequences:

>Translated_435_residues
MTTKPPVHSEQYPQSYYVDTAKEIYAHPQLEGKVSTDVCIIGGGFSGINTAIELRQKGLNVVLLEAKRIGWGASGRNGGE
LIRGIGHDPAQFINEIGQEGVSAIEQMGFEAVEIVRNRIQTHHIDCDLQMGYCDLAVKPRHMLELEQEFDHLKRLGYQHE
IKLLTQSQLGEVIGSDFYQGALVDMGSGHLHPLNLALGEARVARDLGAQLFEYSAAEKIIPRDKPRVLTAKGEVTCQYVV
LAGNAYIGHKLNSYIGGKVLPAGSYLLATEPLTEVQCQSIIPQNMAFADMRIALDYFHLSADRRLLFGGLCTYSGKDPKD
IEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIGRLPDASNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYF
DVFAKIKHMTFPGGPTFRSPMLAAGMLYHRFKDLF
>Mature_434_residues
TTKPPVHSEQYPQSYYVDTAKEIYAHPQLEGKVSTDVCIIGGGFSGINTAIELRQKGLNVVLLEAKRIGWGASGRNGGEL
IRGIGHDPAQFINEIGQEGVSAIEQMGFEAVEIVRNRIQTHHIDCDLQMGYCDLAVKPRHMLELEQEFDHLKRLGYQHEI
KLLTQSQLGEVIGSDFYQGALVDMGSGHLHPLNLALGEARVARDLGAQLFEYSAAEKIIPRDKPRVLTAKGEVTCQYVVL
AGNAYIGHKLNSYIGGKVLPAGSYLLATEPLTEVQCQSIIPQNMAFADMRIALDYFHLSADRRLLFGGLCTYSGKDPKDI
EAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIGRLPDASNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYFD
VFAKIKHMTFPGGPTFRSPMLAAGMLYHRFKDLF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=41.371158392435, Blast_Score=351, Evalue=5e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47936; Mature: 47805

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKPPVHSEQYPQSYYVDTAKEIYAHPQLEGKVSTDVCIIGGGFSGINTAIELRQKGLN
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
VVLLEAKRIGWGASGRNGGELIRGIGHDPAQFINEIGQEGVSAIEQMGFEAVEIVRNRIQ
EEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
THHIDCDLQMGYCDLAVKPRHMLELEQEFDHLKRLGYQHEIKLLTQSQLGEVIGSDFYQG
HEECCEEEEECEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCC
ALVDMGSGHLHPLNLALGEARVARDLGAQLFEYSAAEKIIPRDKPRVLTAKGEVTCQYVV
CEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEE
LAGNAYIGHKLNSYIGGKVLPAGSYLLATEPLTEVQCQSIIPQNMAFADMRIALDYFHLS
EECCEEECCCHHHHCCCEEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHECC
ADRRLLFGGLCTYSGKDPKDIEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEEECCCCCCCCC
RLPDASNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYFDVFAKIKHMTFPGGPTFRSP
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCH
MLAAGMLYHRFKDLF
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKPPVHSEQYPQSYYVDTAKEIYAHPQLEGKVSTDVCIIGGGFSGINTAIELRQKGLN
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
VVLLEAKRIGWGASGRNGGELIRGIGHDPAQFINEIGQEGVSAIEQMGFEAVEIVRNRIQ
EEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
THHIDCDLQMGYCDLAVKPRHMLELEQEFDHLKRLGYQHEIKLLTQSQLGEVIGSDFYQG
HEECCEEEEECEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCC
ALVDMGSGHLHPLNLALGEARVARDLGAQLFEYSAAEKIIPRDKPRVLTAKGEVTCQYVV
CEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEE
LAGNAYIGHKLNSYIGGKVLPAGSYLLATEPLTEVQCQSIIPQNMAFADMRIALDYFHLS
EECCEEECCCHHHHCCCEEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHECC
ADRRLLFGGLCTYSGKDPKDIEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEEECCCCCCCCC
RLPDASNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYFDVFAKIKHMTFPGGPTFRSP
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCH
MLAAGMLYHRFKDLF
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]