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The map label for this gene is cca

Identifier: 117921514

GI number: 117921514

Start: 3689642

End: 3690892

Strand: Direct

Name: cca

Synonym: Shewana3_3076

Alternate gene names: 117921514

Gene position: 3689642-3690892 (Clockwise)

Preceding gene: 117921511

Following gene: 117921515

Centisome position: 74.21

GC content: 50.12

Gene sequence:

>1251_bases
GTGAAAATTTATTTAGTGGGCGGCGCTGTACGAGACAGCCTGCTCAATCTCCCAATAAAAGATAAAGATTTTATGGTTGT
CGGTGCAACTCCAGAGCAAATGCAGCAGTTAGGCTATCGCCAAGTGGGTAAAGACTTCCCAGTTTTTTTGCATCCTAAAA
CCCAACAAGAATATGCCTTAGCGCGAACGGAGCGCAAAGTCGGTCTCGGCTATGGCGGCTTTAGCTGTTATGCCAGTCCG
GACGTCACCCTAGAGCAAGACCTGCTGAGACGCGATTTAACCATTAATGCCATCGCCCAAGATGAGGCGGGTAACTTGTA
CGATCCCTACCATGGCATCGCAGATATCAACGCGCGTCAGCTGCGACATGTGTCGGCCGCCTTTGCTGAAGATCCGCTGC
GAGTATTAAGGGTTGCTCGCTTTGCCGCACGTTTTCATGACTTAGGTTTCGAGATTGCCGCTGAAACCATGGCGTTAATG
CAGCACATGAGCCAAACCGAGGAGTTAACAGCGCTGACGCCTGAGCGCGTCTGGCAAGAAGTCGATAAGAGTCTTGGCGG
CCCTCACCCAGAAGTCTTTTTCGAAGTGCTACGTCAATGTGGCGCCTTGAATATTCTGTTCCCTGAGATTGAGGCGCTTT
TTGGCGTGCCACAACCGGAAAAATGGCATCCAGAGATAGACACGGGTTTGCATACTATGTTGGTGTTAGCCCAAGCTAGC
TCACTGACAGAGGAAAAAGCCGTGCGCTTTGCCGCCTTAGTGCATGACTTGGGTAAAGCATTAAGCCCTAAAGAGCATTG
GCCTAAACACCATGGGCACGGACAAAAGGGCTTACCCGTCATAAAAAGTCTGTGTGAACGCCTGCGGATCCCGAACGAGT
ATCGCGATCTGGCGCTCTTGGTGAGCGATCAGCATCAGAATGTGCACCAAGCTTTTGAGTTAAGAAGCGAGACCATCATC
AAGCTCTTCGATAAAGCCGATTTTTGGCGTAAACCAGAACGACTGGAGCAACTTCTATTAGCCTGCATCGCCGATATGCG
TGGCCGAACCGGATTCGAGCATCATGCCTATCCACAAGGTGATTATCTTTCTGCTTGTTTTTCAGCCGCGAGTTCAGTCG
ATGTGAAGGCTATTATTGCGGCAGGTTTTCAAGGCGCACAGATAAAACAGGCGCTCAATTCGAAGCGGATAGAGGTCGTT
GAGCAAGTGAAACTCAACTGGCAGCAATCCCAAACCAAACAAACGCCATAA

Upstream 100 bases:

>100_bases
CATGGATGTGACATCCCAATAAATATCTCCATCCAAATTCGCCTCGCCATCGCCTCATCATGTTAAGCTAGCTTTATGAC
ACACTAGCTAAAATATTCCT

Downstream 100 bases:

>100_bases
AATTGTAATATTTTTGTAAATGCGACCACACTTTCTGGTAAATACAGTGCCATTTTTCGATGAAAAAGGCCGATATAACC
GTGAAAAAACGCTTTTAAAA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKIYLVGGAVRDSLLNLPIKDKDFMVVGATPEQMQQLGYRQVGKDFPVFLHPKTQQEYALARTERKVGLGYGGFSCYASP
DVTLEQDLLRRDLTINAIAQDEAGNLYDPYHGIADINARQLRHVSAAFAEDPLRVLRVARFAARFHDLGFEIAAETMALM
QHMSQTEELTALTPERVWQEVDKSLGGPHPEVFFEVLRQCGALNILFPEIEALFGVPQPEKWHPEIDTGLHTMLVLAQAS
SLTEEKAVRFAALVHDLGKALSPKEHWPKHHGHGQKGLPVIKSLCERLRIPNEYRDLALLVSDQHQNVHQAFELRSETII
KLFDKADFWRKPERLEQLLLACIADMRGRTGFEHHAYPQGDYLSACFSAASSVDVKAIIAAGFQGAQIKQALNSKRIEVV
EQVKLNWQQSQTKQTP

Sequences:

>Translated_416_residues
MKIYLVGGAVRDSLLNLPIKDKDFMVVGATPEQMQQLGYRQVGKDFPVFLHPKTQQEYALARTERKVGLGYGGFSCYASP
DVTLEQDLLRRDLTINAIAQDEAGNLYDPYHGIADINARQLRHVSAAFAEDPLRVLRVARFAARFHDLGFEIAAETMALM
QHMSQTEELTALTPERVWQEVDKSLGGPHPEVFFEVLRQCGALNILFPEIEALFGVPQPEKWHPEIDTGLHTMLVLAQAS
SLTEEKAVRFAALVHDLGKALSPKEHWPKHHGHGQKGLPVIKSLCERLRIPNEYRDLALLVSDQHQNVHQAFELRSETII
KLFDKADFWRKPERLEQLLLACIADMRGRTGFEHHAYPQGDYLSACFSAASSVDVKAIIAAGFQGAQIKQALNSKRIEVV
EQVKLNWQQSQTKQTP
>Mature_416_residues
MKIYLVGGAVRDSLLNLPIKDKDFMVVGATPEQMQQLGYRQVGKDFPVFLHPKTQQEYALARTERKVGLGYGGFSCYASP
DVTLEQDLLRRDLTINAIAQDEAGNLYDPYHGIADINARQLRHVSAAFAEDPLRVLRVARFAARFHDLGFEIAAETMALM
QHMSQTEELTALTPERVWQEVDKSLGGPHPEVFFEVLRQCGALNILFPEIEALFGVPQPEKWHPEIDTGLHTMLVLAQAS
SLTEEKAVRFAALVHDLGKALSPKEHWPKHHGHGQKGLPVIKSLCERLRIPNEYRDLALLVSDQHQNVHQAFELRSETII
KLFDKADFWRKPERLEQLLLACIADMRGRTGFEHHAYPQGDYLSACFSAASSVDVKAIIAAGFQGAQIKQALNSKRIEVV
EQVKLNWQQSQTKQTP

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=198, Percent_Identity=28.7878787878788, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789436, Length=404, Percent_Identity=61.1386138613861, Blast_Score=530, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71995920, Length=262, Percent_Identity=26.7175572519084, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281360112, Length=209, Percent_Identity=26.7942583732057, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357337, Length=209, Percent_Identity=26.7942583732057, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_SHESA (A0KZT1)

Other databases:

- EMBL:   CP000469
- RefSeq:   YP_870706.1
- ProteinModelPortal:   A0KZT1
- SMR:   A0KZT1
- STRING:   A0KZT1
- GeneID:   4477791
- GenomeReviews:   CP000469_GR
- KEGG:   shn:Shewana3_3076
- NMPDR:   fig|94122.5.peg.3098
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- PhylomeDB:   A0KZT1
- ProtClustDB:   CLSK957802
- BioCyc:   SSP94122:SHEWANA3_3076-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46829; Mature: 46829

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDSLLNLPIKDKDFMVVGATPEQMQQLGYRQVGKDFPVFLHPKTQQEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKVGLGYGGFSCYASPDVTLEQDLLRRDLTINAIAQDEAGNLYDPYHGIADINARQ
HHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHH
LRHVSAAFAEDPLRVLRVARFAARFHDLGFEIAAETMALMQHMSQTEELTALTPERVWQE
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VDKSLGGPHPEVFFEVLRQCGALNILFPEIEALFGVPQPEKWHPEIDTGLHTMLVLAQAS
HHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
SLTEEKAVRFAALVHDLGKALSPKEHWPKHHGHGQKGLPVIKSLCERLRIPNEYRDLALL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHEEEE
VSDQHQNVHQAFELRSETIIKLFDKADFWRKPERLEQLLLACIADMRGRTGFEHHAYPQG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DYLSACFSAASSVDVKAIIAAGFQGAQIKQALNSKRIEVVEQVKLNWQQSQTKQTP
HHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDSLLNLPIKDKDFMVVGATPEQMQQLGYRQVGKDFPVFLHPKTQQEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKVGLGYGGFSCYASPDVTLEQDLLRRDLTINAIAQDEAGNLYDPYHGIADINARQ
HHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHH
LRHVSAAFAEDPLRVLRVARFAARFHDLGFEIAAETMALMQHMSQTEELTALTPERVWQE
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VDKSLGGPHPEVFFEVLRQCGALNILFPEIEALFGVPQPEKWHPEIDTGLHTMLVLAQAS
HHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
SLTEEKAVRFAALVHDLGKALSPKEHWPKHHGHGQKGLPVIKSLCERLRIPNEYRDLALL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHEEEE
VSDQHQNVHQAFELRSETIIKLFDKADFWRKPERLEQLLLACIADMRGRTGFEHHAYPQG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DYLSACFSAASSVDVKAIIAAGFQGAQIKQALNSKRIEVVEQVKLNWQQSQTKQTP
HHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA