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The map label for this gene is trmD [H]

Identifier: 117921454

GI number: 117921454

Start: 3622075

End: 3622821

Strand: Reverse

Name: trmD [H]

Synonym: Shewana3_3015

Alternate gene names: 117921454

Gene position: 3622821-3622075 (Counterclockwise)

Preceding gene: 117921455

Following gene: 117921453

Centisome position: 72.86

GC content: 50.07

Gene sequence:

>747_bases
ATGTGGTTAGGGGTAATTACCCTGTTCCCAGAAATGTTTCGTGCCGTAACAGACTTTGGGGTTACGGGTCGTGCCGTGAA
GAACGGTCTGCTTGAGTTGCACACGTGGAATCCTCGCGATTTCACCCATGATAGACACAGTACGGTAGATGACAGGCCTT
ACGGCGGTGGTCCCGGAATGTTGATGATGGTGCAACCCTTACGCGATGCCATCCATGCTGCGAAAGCGGCGGCAGGCGAA
GAGGCAAAGGTGATTTACTTGTCACCTCAAGGACGCAAGCTGGATCAGCAAGGCGTTACTGAGTTGGCTGAATCATCCCG
GTTGATTCTGGTGTGTGGTCGATACGAAGGTATTGATGAACGCATCATTCAAACGGAAGTGGATGAAGAATGGTCGGTTG
GGGATTACGTGCTTTCGGGCGGTGAATTACCCGCAATGACCTTGATAGATGCAGTATCGAGATTGGTTCCTGGCGTGCTA
GGAAAACAAGCTTCGGCGGAGCAAGATTCTTTCTCCGACGGTTTACTGGACTGTCCTCATTACACGCGCCCTGAAAGTCT
GGATGGACTCGATGTACCCGCAGTACTGCTAAGTGGCAACCACGAACAAATTAGACTCTGGCGTTTGCAGCAAAGCCTTG
GTAGGACTTTTCTAAGACGGCCAGAATTATTTGAAAATCTAGCTCTGACTGACGAACAATCGACTCTTTTAGCGCAGTTC
GTTGAAGCAATGGACAAGAATGCTTAG

Upstream 100 bases:

>100_bases
GCTTCCCTTTGTCCCAGGGCAATTCATCTTAAAGGTGGATGTCCAAGGTAAACAGATTTTAGTGGATTGGGATCCTGACT
TTTAAGTCGAGGTACAACAT

Downstream 100 bases:

>100_bases
TCGTAGCTCAGTTAATTCTAGAACGGAGTAAGTTATGAACAACATCATTAAAATGCTCAACGATGAGCAAATGAAACAAG
ACGTACCTGCGTTTGGTGCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHSTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
EAKVIYLSPQGRKLDQQGVTELAESSRLILVCGRYEGIDERIIQTEVDEEWSVGDYVLSGGELPAMTLIDAVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLDVPAVLLSGNHEQIRLWRLQQSLGRTFLRRPELFENLALTDEQSTLLAQF
VEAMDKNA

Sequences:

>Translated_248_residues
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHSTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
EAKVIYLSPQGRKLDQQGVTELAESSRLILVCGRYEGIDERIIQTEVDEEWSVGDYVLSGGELPAMTLIDAVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLDVPAVLLSGNHEQIRLWRLQQSLGRTFLRRPELFENLALTDEQSTLLAQF
VEAMDKNA
>Mature_248_residues
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHSTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
EAKVIYLSPQGRKLDQQGVTELAESSRLILVCGRYEGIDERIIQTEVDEEWSVGDYVLSGGELPAMTLIDAVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLDVPAVLLSGNHEQIRLWRLQQSLGRTFLRRPELFENLALTDEQSTLLAQF
VEAMDKNA

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=240, Percent_Identity=81.25, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27490; Mature: 27490

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHSTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGEEAKVIYLSPQGRKLDQQGVTELAESSRLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHH
RIIQTEVDEEWSVGDYVLSGGELPAMTLIDAVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGLDVPAVLLSGNHEQIRLWRLQQSLGRTFLRRPELFENLALTDEQSTLLAQF
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
VEAMDKNA
HHHHHCCC
>Mature Secondary Structure
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHSTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGEEAKVIYLSPQGRKLDQQGVTELAESSRLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHH
RIIQTEVDEEWSVGDYVLSGGELPAMTLIDAVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGLDVPAVLLSGNHEQIRLWRLQQSLGRTFLRRPELFENLALTDEQSTLLAQF
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
VEAMDKNA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA