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The map label for this gene is leuB [H]

Identifier: 117921331

GI number: 117921331

Start: 3460159

End: 3461169

Strand: Direct

Name: leuB [H]

Synonym: Shewana3_2890

Alternate gene names: 117921331

Gene position: 3460159-3461169 (Clockwise)

Preceding gene: 117921329

Following gene: 117921336

Centisome position: 69.59

GC content: 48.66

Gene sequence:

>1011_bases
ATGTCAAAAAGAACGATAACCGTAATTCCAGGTGATGGAATTGGCCCAAGTATTATTGATTCAGCCTTAAAAATTCTTGA
TAAAGCAGGTTGTGATTTTGAATATGAATTTGCCGATGCCGGCTTAACTGCTCTCGAGAAGCAGGGCGAACTACTTCCGC
AGCGTACTTTAGAACTGATCGAAAAAAACCGCATCACCCTGAAAGGCCCGCTGACTACACCAGTAGGCGAAGGTTTCACC
TCTATCAACGTGACATTACGTAAGAAATTCGGCCTGTACGCTAACGTGCGTCCAGTGTTGTCTTTCAAAGGCACTCAAGC
CCGTTATGAAAACATTGATATCATTACTGTGCGTGAAAACACCGAAGGCATGTACTCAGGCCACGGTCAAAAAGTGTCTG
AAGACGGCACCACAGCTGAAGCGACCAGTATCGTCACTCGCCAAGGCGCTGAGCAGATCGCGACATTCGCCTATGAACTG
GCCCGCAAAGAAAACCGTAAGAAAGTCACTATCGTGCACAAAGCAAACATCATGAAGTCAACTTCAGGTCTGTTCCTGAA
AGTGGCCCGAGAAGTGAGTCAACGCTACCCAGACATCAAAACTGAAGAAATGATCGTCGATGCGACTTGCATGAAGTTAG
TGATGAACCCAGAAAACTTCGACGTCATCGTGACCACCAACCTGTTCGGTGACATTCTGTCGGATCTGTGTGCTGGTTTA
GTCGGTGGTTTAGGTATGGCTCCTGGCGCCAACATTGGCCGTGATGCAGCGATTTTCGAAGCGGTTCACGGCAGCGCGCC
AGATATCGCAGGTAAAAACCTCGCGAACCCAACATCTGTGATCTTAGCCTCTATCCAAATGCTGGAATACTTAGGCATGG
CCGATAAAGCCGATCTGATCCGTAAAGCCGTATCGGCAGTGATTGAAGAAGGCGACCGTACGACTCGCGATTTAGGCGGC
ACCCACGGTACGACTGACTTCACTCAAGCGGTGCTTGACCGTCTGTCATAA

Upstream 100 bases:

>100_bases
AAAATAATGGGGAGAAAAAACACCAAATTCATAGTCATGTTACCCGGCTAATCCCGGGTCGGACAATTGCAGTAACGACG
AACCGTGGAAGGACTTCTCC

Downstream 100 bases:

>100_bases
TCGCTTTGCGCGAAACAAAAAACGCACCCTAGGGTGCGTTTTTTTATGGCCGCAATCTAGCAGAACAGCCAAATAAAAAG
CCCCAGACATGACTTGGGGC

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPQRTLELIEKNRITLKGPLTTPVGEGFT
SINVTLRKKFGLYANVRPVLSFKGTQARYENIDIITVRENTEGMYSGHGQKVSEDGTTAEATSIVTRQGAEQIATFAYEL
ARKENRKKVTIVHKANIMKSTSGLFLKVAREVSQRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKADLIRKAVSAVIEEGDRTTRDLGG
THGTTDFTQAVLDRLS

Sequences:

>Translated_336_residues
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPQRTLELIEKNRITLKGPLTTPVGEGFT
SINVTLRKKFGLYANVRPVLSFKGTQARYENIDIITVRENTEGMYSGHGQKVSEDGTTAEATSIVTRQGAEQIATFAYEL
ARKENRKKVTIVHKANIMKSTSGLFLKVAREVSQRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKADLIRKAVSAVIEEGDRTTRDLGG
THGTTDFTQAVLDRLS
>Mature_335_residues
SKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPQRTLELIEKNRITLKGPLTTPVGEGFTS
INVTLRKKFGLYANVRPVLSFKGTQARYENIDIITVRENTEGMYSGHGQKVSEDGTTAEATSIVTRQGAEQIATFAYELA
RKENRKKVTIVHKANIMKSTSGLFLKVAREVSQRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGLV
GGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKADLIRKAVSAVIEEGDRTTRDLGGT
HGTTDFTQAVLDRLS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=47.1641791044776, Blast_Score=322, Evalue=2e-88,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=41.9642857142857, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI28178821, Length=336, Percent_Identity=39.8809523809524, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=43.2258064516129, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI28178816, Length=336, Percent_Identity=40.1785714285714, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=47.4747474747475, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI87081683, Length=338, Percent_Identity=38.1656804733728, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=34.5505617977528, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787381, Length=361, Percent_Identity=34.3490304709141, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71986051, Length=332, Percent_Identity=46.3855421686747, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25144293, Length=338, Percent_Identity=41.1242603550296, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=40.4692082111437, Blast_Score=240, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=39.7014925373134, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324709, Length=343, Percent_Identity=45.1895043731778, Blast_Score=293, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=44.5103857566766, Blast_Score=262, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=39.8280802292264, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=29.6398891966759, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=45.1807228915663, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=45.1807228915663, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24661184, Length=339, Percent_Identity=42.7728613569322, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI161078637, Length=337, Percent_Identity=39.4658753709199, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI161078635, Length=337, Percent_Identity=39.4658753709199, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI161078633, Length=337, Percent_Identity=39.4658753709199, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24650122, Length=337, Percent_Identity=39.4658753709199, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI161078639, Length=332, Percent_Identity=39.7590361445783, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=40.8163265306122, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=40.8163265306122, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI20130355, Length=331, Percent_Identity=29.0030211480363, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45550436, Length=223, Percent_Identity=25.1121076233184, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36258; Mature: 36127

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPQRTLELI
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHCCCCCHHHHHHHH
EKNRITLKGPLTTPVGEGFTSINVTLRKKFGLYANVRPVLSFKGTQARYENIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCHHCCEEEEEEECC
TEGMYSGHGQKVSEDGTTAEATSIVTRQGAEQIATFAYELARKENRKKVTIVHKANIMKS
CCCCCCCCCCEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSQRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKADLI
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RKAVSAVIEEGDRTTRDLGGTHGTTDFTQAVLDRLS
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPQRTLELI
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHCCCCCHHHHHHHH
EKNRITLKGPLTTPVGEGFTSINVTLRKKFGLYANVRPVLSFKGTQARYENIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCHHCCEEEEEEECC
TEGMYSGHGQKVSEDGTTAEATSIVTRQGAEQIATFAYELARKENRKKVTIVHKANIMKS
CCCCCCCCCCEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSQRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKADLI
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RKAVSAVIEEGDRTTRDLGGTHGTTDFTQAVLDRLS
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]