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The map label for this gene is anfA [H]

Identifier: 117921290

GI number: 117921290

Start: 3410403

End: 3411812

Strand: Reverse

Name: anfA [H]

Synonym: Shewana3_2849

Alternate gene names: 117921290

Gene position: 3411812-3410403 (Counterclockwise)

Preceding gene: 117921293

Following gene: 117921287

Centisome position: 68.62

GC content: 51.77

Gene sequence:

>1410_bases
ATGATCCGACCAACGCTTTTCTATCATCTACAGGACTTCGAGACCGAGCAGGCATTAGTACAGCTCGAGGGCGCGCAGAC
CTACAACAAGCTACGCAGTACCGATGATTTATCTTGGATTAGTCAGCTGGAGGCCAATCGTATCGATGTGGCGATCATTG
AGCTGCCCCGTCTAAGCCGTGAAGATTACTTAGCCTTACTCGAGTCCAGCGCCATTGCGGATGTGGAGTTTATTTTCCTC
TCCGATGGCCTACCGAATCCTCATCTCGATAAACTCTTATCCAAATACGCGGGCTACCATTTCCGTAAGCCCATCGATAT
GTCGTTGCTGTGCGAAACCTTAGCGGATTTTGCCCAGCATTTGGTTGCCAACACTTCGACGCGCATTCGCCCCTTTGCCA
GCGAATTAGACCAATATGGTCTGCTGGTGGGCTCCTCGTTACCTATGCACCGCTTGTATCGCACTATCCGCCGGGTGTCG
GCCGCCGAGAGCAATGTGTTGATTATCGGCGAGAGTGGCGCGGGTAAAGAACTGGTCGCCAATACCATTCACTTAGCCAG
CCCGCGGGTGAATAAGCCCTATATTGCGATTAACTGTGGCGCCTTGAGCCCTGAATTGGTTGATAGTGAACTCTTCGGCC
ATGTAAAAGGCTCTTTCACTGGCGCGAATCGCGATCATCAAGGGGTATTTGAGCAGGCCGAAGGCGGCACCTTATTCCTC
GATGAAGTCACCGAAATGCCCCTAGAGCATCAAGTTAAGCTGTTAAGAGTGCTCGAAAATAATGAATATCGTCCCGTCGG
CAGCCCTAAGGTGCTCAAGGCCAATGTGCGTATTGTGGCGGCGACAAACCGCGATCCTTTAGTGGCGATTGAGCAAGGAC
AACTGCGGGAGGATTTGTACTTTCGCTTAGCGCATTTCCCCATTCAAGTGCCGCCATTGCGGGAGCGTGGGGAAGATATT
GTCGGACTTGCGAAGCATTTTCTGGCCTATCGCAATGCGGCGGAAAAACAGAGCAAAGTGTTCTCGCCCTCAAGTTTAGA
GGCGATTGCGGCTCACGCTTGGCCGGGGAATGTGCGCGAGTTAAAGCATGCTATCGAGCGCGCTTATATTCTGGCGGACC
ATGAGATTACCCCTGAGCATTTACAGCTTACGCCCAGTTTGGATAAAGAGGCGGCTGCGGAGGAAAATGTCGTGATCCCC
CAAGGTATGCGCCTTGAGGAGCTGGAAAAAATCGCCATTTACCAAGCGCTTGAAACAAGTCAGGGCAACAAGACCGACAC
CGCCGAGCAGCTTGGGATCAGCGTTAAAACCCTCTATAACAAGCTCAGTAAATATGAGCAGGAGTCAGATGCTCAAACTG
TTGTTGATGCCGTTAGCGAAGATTCCGTTAGCAATAGCGTCGCCAAGTAG

Upstream 100 bases:

>100_bases
CGCCAGTGCCTCGCTTATTATCGGCAACTCTTACCGTTGAGATTGTTAAAATTTCACGGCTGGCATACAGTGGTGCTTCA
TTCACAGGGAGTGGCACAAG

Downstream 100 bases:

>100_bases
TGACCTACATAAAATGCCTGAAAATAAGCTAATGCATTGATTCAGATTTGATTCATAGGTTGATTCAGAGTTGATTTTAA
AAGTGTTAGCTTATTTTGGA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MIRPTLFYHLQDFETEQALVQLEGAQTYNKLRSTDDLSWISQLEANRIDVAIIELPRLSREDYLALLESSAIADVEFIFL
SDGLPNPHLDKLLSKYAGYHFRKPIDMSLLCETLADFAQHLVANTSTRIRPFASELDQYGLLVGSSLPMHRLYRTIRRVS
AAESNVLIIGESGAGKELVANTIHLASPRVNKPYIAINCGALSPELVDSELFGHVKGSFTGANRDHQGVFEQAEGGTLFL
DEVTEMPLEHQVKLLRVLENNEYRPVGSPKVLKANVRIVAATNRDPLVAIEQGQLREDLYFRLAHFPIQVPPLRERGEDI
VGLAKHFLAYRNAAEKQSKVFSPSSLEAIAAHAWPGNVRELKHAIERAYILADHEITPEHLQLTPSLDKEAAAEENVVIP
QGMRLEELEKIAIYQALETSQGNKTDTAEQLGISVKTLYNKLSKYEQESDAQTVVDAVSEDSVSNSVAK

Sequences:

>Translated_469_residues
MIRPTLFYHLQDFETEQALVQLEGAQTYNKLRSTDDLSWISQLEANRIDVAIIELPRLSREDYLALLESSAIADVEFIFL
SDGLPNPHLDKLLSKYAGYHFRKPIDMSLLCETLADFAQHLVANTSTRIRPFASELDQYGLLVGSSLPMHRLYRTIRRVS
AAESNVLIIGESGAGKELVANTIHLASPRVNKPYIAINCGALSPELVDSELFGHVKGSFTGANRDHQGVFEQAEGGTLFL
DEVTEMPLEHQVKLLRVLENNEYRPVGSPKVLKANVRIVAATNRDPLVAIEQGQLREDLYFRLAHFPIQVPPLRERGEDI
VGLAKHFLAYRNAAEKQSKVFSPSSLEAIAAHAWPGNVRELKHAIERAYILADHEITPEHLQLTPSLDKEAAAEENVVIP
QGMRLEELEKIAIYQALETSQGNKTDTAEQLGISVKTLYNKLSKYEQESDAQTVVDAVSEDSVSNSVAK
>Mature_469_residues
MIRPTLFYHLQDFETEQALVQLEGAQTYNKLRSTDDLSWISQLEANRIDVAIIELPRLSREDYLALLESSAIADVEFIFL
SDGLPNPHLDKLLSKYAGYHFRKPIDMSLLCETLADFAQHLVANTSTRIRPFASELDQYGLLVGSSLPMHRLYRTIRRVS
AAESNVLIIGESGAGKELVANTIHLASPRVNKPYIAINCGALSPELVDSELFGHVKGSFTGANRDHQGVFEQAEGGTLFL
DEVTEMPLEHQVKLLRVLENNEYRPVGSPKVLKANVRIVAATNRDPLVAIEQGQLREDLYFRLAHFPIQVPPLRERGEDI
VGLAKHFLAYRNAAEKQSKVFSPSSLEAIAAHAWPGNVRELKHAIERAYILADHEITPEHLQLTPSLDKEAAAEENVVIP
QGMRLEELEKIAIYQALETSQGNKTDTAEQLGISVKTLYNKLSKYEQESDAQTVVDAVSEDSVSNSVAK

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=346, Percent_Identity=43.0635838150289, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1788550, Length=316, Percent_Identity=41.7721518987342, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1788905, Length=352, Percent_Identity=39.4886363636364, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=41.4330218068536, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=40.7523510971787, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=42.3197492163009, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=39.7590361445783, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789087, Length=316, Percent_Identity=39.5569620253165, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=38.3435582822086, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=38.125, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=320, Percent_Identity=32.8125, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789828, Length=234, Percent_Identity=32.0512820512821, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=29, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52268; Mature: 52268

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPTLFYHLQDFETEQALVQLEGAQTYNKLRSTDDLSWISQLEANRIDVAIIELPRLSR
CCCCEEEEEEHHCCHHHHHHEECCHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCH
EDYLALLESSAIADVEFIFLSDGLPNPHLDKLLSKYAGYHFRKPIDMSLLCETLADFAQH
HHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVANTSTRIRPFASELDQYGLLVGSSLPMHRLYRTIRRVSAAESNVLIIGESGAGKELVA
HHHCCCCCCCHHHHHHHHHCEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
NTIHLASPRVNKPYIAINCGALSPELVDSELFGHVKGSFTGANRDHQGVFEQAEGGTLFL
HHHEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEE
DEVTEMPLEHQVKLLRVLENNEYRPVGSPKVLKANVRIVAATNRDPLVAIEQGQLREDLY
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCHHHHHH
FRLAHFPIQVPPLRERGEDIVGLAKHFLAYRNAAEKQSKVFSPSSLEAIAAHAWPGNVRE
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCHHH
LKHAIERAYILADHEITPEHLQLTPSLDKEAAAEENVVIPQGMRLEELEKIAIYQALETS
HHHHHHHHEEEECCCCCHHHEEECCCCCHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCC
QGNKTDTAEQLGISVKTLYNKLSKYEQESDAQTVVDAVSEDSVSNSVAK
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRPTLFYHLQDFETEQALVQLEGAQTYNKLRSTDDLSWISQLEANRIDVAIIELPRLSR
CCCCEEEEEEHHCCHHHHHHEECCHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCH
EDYLALLESSAIADVEFIFLSDGLPNPHLDKLLSKYAGYHFRKPIDMSLLCETLADFAQH
HHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVANTSTRIRPFASELDQYGLLVGSSLPMHRLYRTIRRVSAAESNVLIIGESGAGKELVA
HHHCCCCCCCHHHHHHHHHCEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
NTIHLASPRVNKPYIAINCGALSPELVDSELFGHVKGSFTGANRDHQGVFEQAEGGTLFL
HHHEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEE
DEVTEMPLEHQVKLLRVLENNEYRPVGSPKVLKANVRIVAATNRDPLVAIEQGQLREDLY
HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCHHHHHH
FRLAHFPIQVPPLRERGEDIVGLAKHFLAYRNAAEKQSKVFSPSSLEAIAAHAWPGNVRE
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCHHH
LKHAIERAYILADHEITPEHLQLTPSLDKEAAAEENVVIPQGMRLEELEKIAIYQALETS
HHHHHHHHEEEECCCCCHHHEEECCCCCHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCC
QGNKTDTAEQLGISVKTLYNKLSKYEQESDAQTVVDAVSEDSVSNSVAK
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]