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The map label for this gene is purU [H]

Identifier: 117921262

GI number: 117921262

Start: 3369927

End: 3370829

Strand: Reverse

Name: purU [H]

Synonym: Shewana3_2821

Alternate gene names: 117921262

Gene position: 3370829-3369927 (Counterclockwise)

Preceding gene: 117921263

Following gene: 117921261

Centisome position: 67.79

GC content: 48.5

Gene sequence:

>903_bases
ATGAGGGACCATAGAGGAGATCAGATTTTGCAGTCACAGCATGAAGTAGTATCGAACGCGCAACCCGCCTTAGCACGTAA
AATCCTGATGACAGATTGCGCCGATGCCCAAGGGCTTATCGCCAAAATCACCAGTGTTTGCTTCAATCATCAGCTTAACA
TCATTAAAAACAGTGAGTTTGTGGATAATGCTCAGGGGCGATTTTTTATGCGTACTGAGCTCGAAGGGCACTTTAATAGC
GAGCAATTACTGCAGGATCTGCGGGAGGTGTTACCCGCGCAAAATCATATGACCTTGGTCAGCGCGGGCAAGAAGCGCAT
CGTCGTGTTAGTCACCAAAGAGGCCCATTGTTTGGGCGATTTGCTGATGAAGGCCTACTATGGCGGCTTGAATGTTGAGA
TAGCAGCCGTGGTGGGCAACCATGATGTGTTAAGGGAATTGGTTGAAAAATTTGATATTCCTTTTCACCTCGTGAGTCAC
GAAGGGCTCGACAGAATTCAGCATGAGCAGGCGTTATTGGCCGCTGTGTCGCAATACGCGCCCGATTATCTGGTGCTCGC
CAAGTATATGCGAGTGCTGACGCCGGATTTTGTCGCCGAATATCCAAACCGTATCATCAATATCCATCATTCGTTCTTAC
CTGCCTTTATCGGTGCCGCACCTTATCGCCAAGCTTGGGAGCGAGGAGTGAAAATCATCGGTGCGACGGCACATTTTGTG
AATAATTGCCTCGATGAAGGGCCGATCATTAAGCAGGACGTGATCCCCGTCGATCACAGTTACAGCGCGCTTGAAATGGC
CAAAGCGGGGCGTGATGTCGAAAAAAGCGTCCTCAGTAAAGCCTTACAGCTAGTGCTAAATGAGCAAGTAGTGGTTTACG
GCAATAAAACCATAGTGTTTTAA

Upstream 100 bases:

>100_bases
TTTATTGTGTTTAATAAAGTTAACTCACAATAAATTCACTTTTTATCACAAAAAATAAGCTTTTCATGCTGTATGAGTGG
ATTGGCTATGGCACACTCGA

Downstream 100 bases:

>100_bases
CGTCAGTTAAAAGTGACAGGCAAGTAGGCTTTAACTGCTTGTCTGTTAAGACTTTAGAATTAAGCCTAGGCACTGCGCTT
GCTATCAGTATGTTTAATTG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRDHRGDQILQSQHEVVSNAQPALARKILMTDCADAQGLIAKITSVCFNHQLNIIKNSEFVDNAQGRFFMRTELEGHFNS
EQLLQDLREVLPAQNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLNVEIAAVVGNHDVLRELVEKFDIPFHLVSH
EGLDRIQHEQALLAAVSQYAPDYLVLAKYMRVLTPDFVAEYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFV
NNCLDEGPIIKQDVIPVDHSYSALEMAKAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF

Sequences:

>Translated_300_residues
MRDHRGDQILQSQHEVVSNAQPALARKILMTDCADAQGLIAKITSVCFNHQLNIIKNSEFVDNAQGRFFMRTELEGHFNS
EQLLQDLREVLPAQNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLNVEIAAVVGNHDVLRELVEKFDIPFHLVSH
EGLDRIQHEQALLAAVSQYAPDYLVLAKYMRVLTPDFVAEYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFV
NNCLDEGPIIKQDVIPVDHSYSALEMAKAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF
>Mature_300_residues
MRDHRGDQILQSQHEVVSNAQPALARKILMTDCADAQGLIAKITSVCFNHQLNIIKNSEFVDNAQGRFFMRTELEGHFNS
EQLLQDLREVLPAQNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLNVEIAAVVGNHDVLRELVEKFDIPFHLVSH
EGLDRIQHEQALLAAVSQYAPDYLVLAKYMRVLTPDFVAEYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFV
NNCLDEGPIIKQDVIPVDHSYSALEMAKAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=200, Percent_Identity=29, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI209869995, Length=200, Percent_Identity=29, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI209869993, Length=200, Percent_Identity=29, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=68.3453237410072, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788846, Length=93, Percent_Identity=44.0860215053763, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=31.5217391304348, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24582400, Length=199, Percent_Identity=27.6381909547739, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33599; Mature: 33599

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDHRGDQILQSQHEVVSNAQPALARKILMTDCADAQGLIAKITSVCFNHQLNIIKNSEF
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCH
VDNAQGRFFMRTELEGHFNSEQLLQDLREVLPAQNHMTLVSAGKKRIVVLVTKEAHCLGD
HCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCHHHHHH
LLMKAYYGGLNVEIAAVVGNHDVLRELVEKFDIPFHLVSHEGLDRIQHEQALLAAVSQYA
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHC
PDYLVLAKYMRVLTPDFVAEYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFV
CHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHH
NNCLDEGPIIKQDVIPVDHSYSALEMAKAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF
HHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCEEEC
>Mature Secondary Structure
MRDHRGDQILQSQHEVVSNAQPALARKILMTDCADAQGLIAKITSVCFNHQLNIIKNSEF
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCH
VDNAQGRFFMRTELEGHFNSEQLLQDLREVLPAQNHMTLVSAGKKRIVVLVTKEAHCLGD
HCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCHHHHHH
LLMKAYYGGLNVEIAAVVGNHDVLRELVEKFDIPFHLVSHEGLDRIQHEQALLAAVSQYA
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHC
PDYLVLAKYMRVLTPDFVAEYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFV
CHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHH
NNCLDEGPIIKQDVIPVDHSYSALEMAKAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF
HHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]